Selection and Application of ssDNA Aptamers for Fluorescence Biosensing Detection of Malachite Green

Xie, Miaojia; Chen, Zanlin; Zhao, Fang; Lin, Ying; Zheng, Suiping; Han, Sang‐Ik

doi:10.3390/foods11060801

Cited by 11 publications

(4 citation statements)

References 33 publications

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“…In addition, their secondary structural requirements have been investigated through thermodynamic and mutation studies. The 2.8 A crystal structure of the malachite green aptamer and the structural investigations of RNA and DNA aptamers in solution have been described in detail in previous studies [61][62][63][64][65][66][67][68][69][70].…”

Section: Dna Aptamersmentioning

confidence: 99%

Current State of Human Gene Therapy: Approved Products and Vectors

Shchaslyvyi,

Antonenko,

Tesliuk

et al. 2023

Pharmaceuticals

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In the realm of gene therapy, a pivotal moment arrived with Paul Berg’s groundbreaking identification of the first recombinant DNA in 1972. This achievement set the stage for future breakthroughs. Conditions once considered undefeatable, like melanoma, pancreatic cancer, and a host of other ailments, are now being addressed at their root cause—the genetic level. Presently, the gene therapy landscape stands adorned with 22 approved in vivo and ex vivo products, including IMLYGIC, LUXTURNA, Zolgensma, Spinraza, Patisiran, and many more. In this comprehensive exploration, we delve into a rich assortment of 16 drugs, from siRNA, miRNA, and CRISPR/Cas9 to DNA aptamers and TRAIL/APO2L, as well as 46 carriers, from AAV, AdV, LNPs, and exosomes to naked mRNA, sonoporation, and magnetofection. The article also discusses the advantages and disadvantages of each product and vector type, as well as the current challenges faced in the practical use of gene therapy and its future potential.

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Section: Dna Aptamersmentioning

confidence: 99%

Current State of Human Gene Therapy: Approved Products and Vectors

Shchaslyvyi,

Antonenko,

Tesliuk

et al. 2023

Pharmaceuticals

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“…The fluorescence intensity will be recovered when the labeled aptamer is desorbed from the surface of GO in the presence of small-molecule target. The fluorescence intensity change are measured using a microplate reader, and eventually the K d value of the aptamer for its small-molecule target is calculated [ 72 , 77 , 109 ].…”

Section: Small-molecule–aptamer Interaction and Characterizationmentioning

confidence: 99%

“…The ITC assay is a label-free and immobilization-free assay for measuring the binding affinity of an aptamer for its small-molecule target [ 44 , 83 ] in an instrument comprising a reference cell and a sample cell ( Figure 2 F). The reference cell is filled with a buffer and water (without the aptamer or its small-molecule target), while the sample cell is filled with the buffer and the aptamer and is titrated via syringe with up to 20 volumes of the aptamer’s small-molecule target [ 77 ]. The temperature in the two cell units is maintained at a constant value, and when the small molecule is titrated against the aptamer in the sample cell, an exothermic binding reaction occurs.…”

Section: Small-molecule–aptamer Interaction and Characterizationmentioning

confidence: 99%

Capture-SELEX: Selection Strategy, Aptamer Identification, and Biosensing Application

2022

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Small-molecule contaminants, such as antibiotics, pesticides, and plasticizers, have emerged as one of the substances most detrimental to human health and the environment. Therefore, it is crucial to develop low-cost, user-friendly, and portable biosensors capable of rapidly detecting these contaminants. Antibodies have traditionally been used as biorecognition elements. However, aptamers have recently been applied as biorecognition elements in aptamer-based biosensors, also known as aptasensors. The systematic evolution of ligands by exponential enrichment (SELEX) is an in vitro technique used to generate aptamers that bind their targets with high affinity and specificity. Over the past decade, a modified SELEX method known as Capture-SELEX has been widely used to generate DNA or RNA aptamers that bind small molecules. In this review, we summarize the recent strategies used for Capture-SELEX, describe the methods commonly used for detecting and characterizing small-molecule–aptamer interactions, and discuss the development of aptamer-based biosensors for various applications. We also discuss the challenges of the Capture-SELEX platform and biosensor development and the possibilities for their future application.

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“…Three aptamers with excellent performance in detecting MG were identified in our previous work. They constructed a fluorescence sensor based on those aptamers, with the LOD being 1.82 ng/mL [ 30 ]. However, this sensor requires the fluorescent modification of the aptamer, which had a relatively low recovery rate for samples with lower concentrations.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Electrochemical Aptasensor for Sensitive Detection of Malachite Green Based on AuNPs/MWCNTs@TiO2 Nanocomposites

Chen,

Li,

Xie

et al. 2023

IJMS

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This study proposes a label-free aptamer biosensor for the sensitive detection of malachite green(MG) using gold nanoparticles/multi-walled carbon nanotubes @ titanium dioxide(AuNPs/MWCNTs@TiO2). The nanocomposite provides a large surface area and good electrical conductivity, improving current transfer and acting as a platform for aptamer immobilization. The aptamer and the complementary chain(cDNA) are paired by base complementary to form the recognition element and fixed on the AuNPs by sulfhydryl group, which was modified on the cDNA. Since DNA is negatively charged, the redox probe in the electrolyte is less exposed to the electrode surface under the repulsion of the negative charge, resulting in a low-electrical signal level. When MG is present, the aptamer is detached from the cDNA and binds to MG, the DNA on the electrode surface is reduced, and the rejection of the redox probe is weakened, which leads to an enhanced electrical signal and enables the detection of MG concentration by measuring the change in the electrical signal. Under the best experimental conditions, the sensor demonstrates a good linear relationship for the detection of MG from 0.01 to 1000 ng/mL, the limit of detection (LOD)is 8.68 pg/mL. This sensor is stable, specific, and reproducible, allowing for the detection of various small-molecule pollutants by changing the aptamer, providing an effective method for detecting small-molecule pollutants.

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Selection and Application of ssDNA Aptamers for Fluorescence Biosensing Detection of Malachite Green

Cited by 11 publications

References 33 publications

Current State of Human Gene Therapy: Approved Products and Vectors

Current State of Human Gene Therapy: Approved Products and Vectors

Capture-SELEX: Selection Strategy, Aptamer Identification, and Biosensing Application

Label-Free Electrochemical Aptasensor for Sensitive Detection of Malachite Green Based on AuNPs/MWCNTs@TiO2 Nanocomposites

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