Selectins Influence Thrombosis in a Mouse Model of Experimental Deep Venous Thrombosis

Myers, Daniel D.; Farris, Diana M.; Hawley, Angela E.; Wrobleski, Shirley K.; Chapman, Amy M.; Stoolman, Lloyd M.; Knibbs, Randy; Strieter, Robert M.; Wakefield, Thomas W.

doi:10.1006/jsre.2002.6552

Cited by 148 publications

(151 citation statements)

References 26 publications

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“…At designated time points after ligation, the thrombus-containing IVC was carefully removed by cutting the IVC proximally, just below the ligature, and distally, at the lower end of the thrombus. As previously reported, [21][22][23] the thrombus constitutes the bulk of the combined weight of thrombus and IVC, and thus the weight of the thrombus-containing IVC is widely used in studies of this model in mice to reflect the weight of clot; the size of the clot is also assessed by the length of the thrombus and the weight/length ratio of the thrombus. Moreover, the thrombus may not be readily separated from the IVC without traumatizing and tearing the clot and the wall of the IVC, and, notably, for reasons that are unclear, this was especially so in HO-1 Ϫ/Ϫ mice.…”

Section: Murine Model Of Deep Venous Thrombosis: Ligation Of the Ivc mentioning

confidence: 95%

“…Using a long-established and widely employed model of venous thrombosis, and one based on the ligation of the infra-renal inferior vena cava (IVC), 4,6,7,[21][22][23] the present study addressed whether expression of HO-1 influences venous thrombosis and clot resolution. Ligation of the IVC induces prompt and prominent venous clot formation, inflammation in the clot and venous wall, and, over ensuing days, increasing resolution of the venous clot.…”

Section: 2mentioning

confidence: 99%

“…This model was performed as described previously by Myers et al [21][22][23] Mice were anesthetized (pentobarbital, 50 mg/kg, i.p.) and were placed on a temperature-regulated table to maintain body temperature at 37°C.…”

Section: Murine Model Of Deep Venous Thrombosis: Ligation Of the Ivc mentioning

confidence: 99%

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Induction of Heme Oxygenase-1 is a Beneficial Response in a Murine Model of Venous Thrombosis

Tracz

Juncos

Grande

et al. 2008

The American Journal of Pathology

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؊/؊ mice exhibited increased nuclear factor B activation and markedly increased up-regulation of tissue factor, selectins, inflammatory cytokines, and matrix metalloproteinase-9, the latter incriminated in both clot lysis and vascular injury. We conclude that HO-1 deficiency impairs thrombus resolution and exaggerates the inflammatory response to thrombus formation. These findings offer insight into recent observations that polymorphisms in the HO-1 gene may increase the risk for recurrent venous thrombosis and dysfunction of hemodialysis arteriovenous fistulas, the latter caused, in part, by thrombosis.

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Section: Murine Model Of Deep Venous Thrombosis: Ligation Of the Ivc mentioning

confidence: 95%

Section: 2mentioning

confidence: 99%

See 1 more Smart Citation

Induction of Heme Oxygenase-1 is a Beneficial Response in a Murine Model of Venous Thrombosis

Tracz

Juncos

Grande

et al. 2008

The American Journal of Pathology

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“…[14,[17][18] The first mice was used for a controlled experiment using blank GNRs, the second was used for the single targeting using anti-ICAM-1 conjugated GNRs (AR1:3), and the third was used for the multiple targeting using mixed anti-ICMA-1 conjugated GNRs (AR1:3) and anti-E-selectin conjugated GNRs (AR1:3.5). All three animals received photothermal injury treatments.…”

Section: B Animal Preparationmentioning

confidence: 99%

Photoacousic Molecular Imaging of Inflammatory Disease

2015

International Academy of Engineers (IA-E) April 24-25, 2015 Pattaya (Thailand)

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Abstract---Multiple biomarkers of cardiovascular inflammatory were simultaneously imaged in vivo using antibody conjugated gold nanorods (GNRs) injected into a mouse model of vascular injury stimulated by a photochemical reaction of Rose Bengal dye to green light. Mixed solutions of ICAM-1 antibody conjugated GNRs (715 nm) and E-selectin antibody conjugated GNRs (800 nm) were injected to bind to their respective inflammatory markers on the luminal surface of the inferior vena cava of a mouse. Photoacoustic intensity was measured by a commercial ultrasound probe synchronized to a pulsed laser (10-18 mJ/cm 2 ) at 715 nm or 800 nm clearly identified the up-regulation of targeted biomarkers. Histopathology on the harvested tissues confirmed inflammation. The feasibility of simultaneous photoacoustic molecular imaging of inflammation responses in cardiovascular system using a commercial ultrasound system has been demonstrated in vivo.

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“…These new findings underscore the crucial role of early endothelial injury during sepsis. The role of endotheliumderived MPs as prothrombotic MPs has previously been described: in a mouse model of deep venous thrombosis, E-selectin, expressed by endothelium-derived MPs, reinforces the thrombotic response [10]. In thrombotic thrombocytopenic purpura, endothelium-derived MPs can express very large von Willebrand factor multimers resulting in platelet aggregation [11].…”

mentioning

confidence: 95%