Selecting suitable image dimensions for scanning probe microscopy

Bowen, James; Cheneler, David

doi:10.1016/j.surfin.2017.09.003

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…It was showed that the measured height of organic samples critically depend on pixel size, as the measured average sample height decreased and standard deviation increased with increasing pixel size [ 108 ]. It was demonstrated that increasing pixel size results in an increase in the apparent surface roughness, and therefore in larger sample height deviations [ 112 , 113 ]. Quite generally, it is clear that imaging at insufficient resolution will cause distortions in image and will lead to deviations or lateral and vertical dimension measurements.…”

Section: Resolution Sensitivity and Accuracy For Single Molecule Meamentioning

confidence: 99%

Atomic force microscopy for single molecule characterisation of protein aggregation

Ruggeri

Šneideris

Vendruscolo

et al. 2019

Archives of Biochemistry and Biophysics

121

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The development of atomic force microscopy (AFM) has opened up a wide range of novel opportunities in nanoscience and new modalities of observation in complex biological systems. AFM imaging has been widely employed to resolve the complex and heterogeneous conformational states involved in protein aggregation at the single molecule scale and shed light onto the molecular basis of a variety of human pathologies, including neurodegenerative disorders. The study of individual macromolecules at nanoscale, however, remains challenging, especially when fully quantitative information is required. In this review, we first discuss the principles of AFM with a special emphasis on the fundamental factors defining its sensitivity and accuracy. We then review the fundamental parameters and approaches to work at the limit of AFM resolution in order to perform single molecule statistical analysis of biomolecules and nanoscale protein aggregates. This single molecule statistical approach has proved to be powerful to unravel the molecular and hierarchical assembly of the misfolded species present transiently during protein aggregation, to visualise their dynamics at the nanoscale, as well to study the structural properties of amyloid-inspired functional nanomaterials.

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Section: Resolution Sensitivity and Accuracy For Single Molecule Meamentioning

confidence: 99%

Atomic force microscopy for single molecule characterisation of protein aggregation

Ruggeri

Šneideris

Vendruscolo

et al. 2019

Archives of Biochemistry and Biophysics

121

View full text Add to dashboard Cite

show abstract

“…It is expected the films will exhibit a high degree of cross-linking, which gives these films their high modulus. This also leads to very smooth films [27] covered with fluorine atoms, thus exhibiting low adhesion and friction [7]. This low friction reduces the tensile stress surrounding the sharp tip, and in turn the yield zone, and should therefore reduce the amount of plastic flow damage [28].…”

Section: Abrasive Wear Tests Using Afmmentioning

confidence: 99%

The stability and degradation of PECVD fluoropolymer nanofilms

Bowen

Cheneler

2019

Polymer Degradation and Stability

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“…The two famous core methods of surface scanning – AFM and NSOM – were developed separately in the 1980s, forty years later the trend is clearly to improve, combine, and adapt methods while reducing efforts and increasing accuracy. Invented in 1986 by IBM scientists 11 , and following its Scanning Tunneling Microscope (STM) predecessor 12 , 13 , the Atomic Force Microscopy (AFM) served for years as the Scanning Probe Microscopy (SPM) branch 14 , 15 core method 16 , mainly used for nanoscale surface topography characterization. On the other hand, the Near Field Scanning Optical Microscopy (NSOM) 17 , served in parallel as the sub-diffractive optical characterization core method 18 .…”

Section: Introductionmentioning

confidence: 99%

Near-field projection optical microscope (NPOM) as a new approach to nanoscale super-resolved imaging

Sanjeev,

Glukhov,

Salahudeen Rafeeka

et al. 2023

Sci Rep

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A new super-resolution method, entitled Near-field Projection Optical Microscopy (NPOM), is presented. This novel technique enables the imaging of nanoscale objects without the need for surface scanning, as is usually required in existing methods such as NSOM (near-field scanning optical microscope). The main advantage of the proposed concept, besides the elimination of the need for a mechanical scanning mechanism, is that the full field of regard/view is imaged simultaneously and not point-by-point as in scanning-based techniques. Furthermore, by using compressed sensing, the number of projected patterns needed to decompose the spatial information of the inspected object can be made smaller than the obtainable points of spatial resolution. In addition to the development of mathematical formalism, this paper presents the results of a series of complementary numerical tests, using various objects and patterns, that were performed to verify the accuracy of the reconstruction capabilities. We have also performed a proof of concept experiment to support the numerical formalism.

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Selecting suitable image dimensions for scanning probe microscopy

Cited by 4 publications

References 18 publications

Atomic force microscopy for single molecule characterisation of protein aggregation

Atomic force microscopy for single molecule characterisation of protein aggregation

The stability and degradation of PECVD fluoropolymer nanofilms

Near-field projection optical microscope (NPOM) as a new approach to nanoscale super-resolved imaging

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