Selecting peptide ligands of microcystin-LR from phage displayed random libraries

Selection of phage-displayed peptides for the detection of imidacloprid in water and soil

“…However, our trials to select cyclic peptides specific for IMI-BSA in vitro were unsuccessful; only linear peptides that specifically bound IMI-BSA were obtained. This result was in accordance with other studies in which linear analogues showed better analyte-targeting properties than their cyclic counterparts [23,31,34]. Probably the conformations of cyclic 7-mer peptides limited the essential flexibility necessary for the recognition for imidacloprid.…”

“…However, because small molecules have less binding surface and are difficult to immobilize, application of the technology directly for small molecules can be challenging. A few studies have successfully employed peptide display to select binders for small molecules, for example, fluorophores [29], 2,4,6-trinitrotoluene [30], microcystin-LR [31], and paclitaxel (Taxol) [32]. We recently presented a panning strategy for the integrated selection of phage-borne peptides binding to either a particular ligand-antibody complex or a ligand-free antibody, leading to the development of assays for the targeted ligand 2,2 0 , 4,4 0 -tetrabromodiphenyl ether (BDE47) [33].…”

Identification of human liver mitochondrial aldehyde dehydrogenase as a potential target for microcystin-LR

“…For instance, McElhiney et al (2000McElhiney et al ( , 2002 isolated antibody fragments from a phage display antibody library that specifically recognized MC-LR, thereby expanding the application of this technology in the study of MCs. In our previous work, we obtained phage clones that bind specifically to microcystin-LR (MC-LR) from both linear and constrained libraries (Zhao et al, 2005).…”

Mimic peptides bonding specifically with the first and second extracellular loops of the CC chemokine receptor 5 derived from a phage display peptide library are potent inhibitors of experimental autoimmune encephalomyelitis

“…The rest of the phage was then tipped out into a micro-centrifuge tube as Round 1 elution, and amplified according to the procedure's protocol. Two more rounds were performed mainly as mentioned above, with washing buffer changed to TBS containing 0.5% Tween 20, and Round 2 elution and Round 3 elution were obtained, respectively [14,15]. In the Round 3 elution, 20 blue clones were randomly selected.…”