Selecting a relevant animal model for testing the in vivo effects of Choukroun's platelet-rich fibrin (PRF): Rabbit tricks and traps

Ehrenfest, David M. Dohan; Lemo, Nikša; Jimbo, Ryo; Sammartino, Gilberto

doi:10.1016/j.tripleo.2010.05.057

Cited by 25 publications

(17 citation statements)

References 12 publications

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“…One of the important considerations in generating a good L-PRF membrane is the time delay between blood draw and centrifugation. The success of L-PRF technique entirely depends on the speed of blood collection and immediate transfer to the centrifuge 19 , usually within a minute. It is impossible to generate a wellstructured L-PRF clot (with its specific cell content, matrix architecture and growth factor release profile), if blood harvesting is prolonged and not homogenous; a small incoherent, friable mass of fibrin with unknown content is formed instead.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial

Madurantakam

Yoganarasimha

Hasan

2015

JoVE

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Autologous platelet concentrates represent promising innovative tools in the field of regenerative medicine and have been extensively used in oral surgery. Unlike platelet rich plasma (PRP) that is a gel or a suspension, Leukocyte-Platelet Rich Fibrin (L-PRF) is a solid 3D fibrin membrane generated chair-side from whole blood containing no anti-coagulant. The membrane has a dense three dimensional fibrin matrix with enriched platelets and abundant growth factors. L-PRF is a popular adjunct in surgeries because of its superior handling characteristics as well as its suturability to the wound bed. The goal of the study is to demonstrate generation as well as provide detailed characterization of relevant properties of L-PRF that underlie its clinical success. Video LinkThe video component of this article can be found at

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Section: Discussionmentioning

confidence: 99%

Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial

Madurantakam

Yoganarasimha

Hasan

2015

JoVE

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“…Indeed Choukroun's PRF shows very different characteristics than the other products: blood is collected without anticoagulant and centrifuged immediately, leading to the natural formation of a strong PRF clot in the middle of the tube (without the addition of any adjuvants or triggering factors). PRF is a solid biomaterial [39][40][41][42], an optimized natural blood clot [43,44], and not an injectable liquid platelet suspension like the PRPs. The platelet activation and the fibrin polymerization are not the final step of the process, they are the process [43].…”

Section: In Search For a Better Terminology: A Slow Evolutionmentioning

confidence: 99%

In Search of a Consensus Terminology in the Field of Platelet Concentrates for Surgical Use: Platelet-Rich Plasma (PRP), Platelet-Rich Fibrin (PRF), Fibrin Gel Polymerization and Leukocytes

Ehrenfest

Bielecki²,

Mishra³

et al. 2012

CPB

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230

180

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In the field of platelet concentrates for surgical use, most products are termed Platelet-Rich Plasma (PRP). Unfortunately, this term is very general and incomplete, leading to many confusions in the scientific database. In this article, a panel of experts discusses this issue and proposes an accurate and simple terminology system for platelet concentrates for surgical use. Four main categories of products can be easily defined, depending on their leukocyte content and fibrin architecture: Pure Platelet-Rich Plasma (P-PRP), such as cell separator PRP, Vivostat PRF or Anitua's PRGF; Leukocyteand Platelet-Rich Plasma (L-PRP), such as Curasan, Regen, Plateltex, SmartPReP, PCCS, Magellan, Angel or GPS PRP; Pure Plaletet-Rich Fibrin (P-PRF), such as Fibrinet; and Leukocyte- and Platelet-Rich Fibrin (L-PRF), such as Choukroun's PRF. P-PRP and L-PRP refer to the unactivated liquid form of these products, their activated versions being respectively named P-PRP gels and L-PRP gels. The purpose of this search for a terminology consensus is to plead for a more serious characterization of these products. Researchers have to be aware of the complex nature of these living biomaterials, in order to avoid misunderstandings and erroneous conclusions. Understanding the biomaterials or believing in the magic of growth factors ? From this choice depends the future of the field.

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“…To create a more challenging environment for bone generation, the periosteum was also resected. The recommended method of blood collection from rabbits is debated and a cardiac puncture is often recommended (37). In the present study, whole blood was harvested from the central ear artery while the rabbit was restrained in a restraining box, a method previously used by Lee and Pripatnanont (59,69).…”

Section: Discussionmentioning

confidence: 99%

“…The periosteum was resected to avoid any influence on bony regeneration. A 15-mm diameter full-thickness defect, which was demonstrated to be the critical-size defect (CSD) in previous studies (36,37), was carefully prepared to avoid dural tears using a dental bar and continuous irrigation with sterile saline at room temperature. Rabbits were subsequently administered with their respective treatments.…”

Section: Methodsmentioning

confidence: 99%

Hybrid composites of mesenchymal stem cell sheets, hydroxyapatite, and platelet-rich fibrin granules for bone regeneration in a rabbit calvarial critical-size defect model

Wang

Guo

et al. 2017

Experimental and Therapeutic Medicine

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The reconstruction of large bone defects remains a major clinical challenge, and tissue engineering is a promising technique for resolving this problem. Many attempts have been made to optimize bone tissue engineering protocols. The aim of the present study was to develop a process incorporating mesenchymal stem cell (MSC) sheets with nanoscale hydroxyapatite (nano-HA) and autologous platelet-rich fibrin (PRF) granules for enhanced bone formation within a critical-sized rabbit cranial defect. MSC sheets and PRF were prepared prior to in vivo experiments. The osteogenic differentiation ability of MSCs and the ultrastructure of PRF were also studied. A total of 15 New Zealand white rabbits were used in the current study and critical-size defects (CSDs) were surgically introduced in the cranium (diameter, 15 mm). The surgical defects were treated with MSC/PRF composites, MSC composites or left empty. Animals were euthanized at week 8 post-surgery. Iconography, histological and histomorphometric analysis were performed to assess de novo bone formation. The percentage of new bone in the MSC/PRF group (35.7±5.1%) was significantly higher than that in the MSC (18.3±3.2%; P<0.05) and empty defect groups (4.7±1.5%; P<0.05). The results of the present study suggest that combined application of an MSC sheet with nano-HA and granular PRF enhances bone regeneration in a rabbit calvarial CSD model, and provides a novel insight into bone tissue regeneration for large bone defects.

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Selecting a relevant animal model for testing the in vivo effects of Choukroun's platelet-rich fibrin (PRF): Rabbit tricks and traps

Cited by 25 publications

References 12 publications

Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial

Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial

In Search of a Consensus Terminology in the Field of Platelet Concentrates for Surgical Use: Platelet-Rich Plasma (PRP), Platelet-Rich Fibrin (PRF), Fibrin Gel Polymerization and Leukocytes

Hybrid composites of mesenchymal stem cell sheets, hydroxyapatite, and platelet-rich fibrin granules for bone regeneration in a rabbit calvarial critical-size defect model

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