Selected recombinant Aspergillus fumigatus allergens bind specifically to IgE in ABPA

Abstract

The results demonstrate that Asp f 2, f 4, and f 6 can be used in the serodiagnosis of ABPA, while IgE antibody binding to Asp f 1 and f 3 was not specific.

Generation of Th1 T cell responses directed to a HLA Class II restricted epitope from theAspergillusf16 allergen

“…allergen.org). Most of the identified allergens elicit humoral immune responses [26][27][28][29], others are toxic to PBMC or are strongly homologous to human proteins, while some appear to prime primarily a Th2 T cell response [30][31][32][33]. Two allergens have been shown to stimulate both T and B cell responses from patients with ABPA, Asp f2 and Asp f16 [34,35].…”

Immune response to n-terminal and c-terminal deletion mutants of Aspergillus fumigatus major allergen ASP F 3

“…The involvement of Af-specific Th1 cytokines in allergic response raise the possibility of uncovering effective therapeutic modalities that would promote Th1 immune response and /or T-cell tolerance through regulatory T cells (CD4+ CD25+) in Af sensitized patients. In recent years, major Af allergens have been identified and purified using molecular cloning and expression protocol (3)(4)(5)(6). Asp f 3 has been recognized as a major allergen of Af, which elicited immediate wheal and flare skin reactions and strong IgE binding with a majority of ABPA patients' sera (1,4,7).…”

Diagnóstico de ABPA em pacientes portadores de fibrose cística: utilidade clínica da pesquisa de IgE específica contra alérgenos recombinantes do Aspergillus fumigatus

“…It has been demonstrated that patients with ABPA react to a larger number of the recombinant allergens in this panel, some of which r Asp f2, f4 and f6 are considered specific to ABPA patients. [15][16][17][18] This technique therefore appeared to be very promising for ABPA diagnosis.…”