Selected lactobacilli strains inhibit inflammation in LPS-induced RAW264.7 macrophages by suppressing the TLR4-mediated NF-κB and MAPKs activation

Shi, Jialu; LI, Huizhen; Liang, Shengnan; Evivie, Smith Etareri; Gui-cheng, Huo; LI, Bailiang; Yin, Liu

doi:10.1590/fst.107621

Cited by 13 publications

(7 citation statements)

References 36 publications

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“…To prevent or treat inflammatory diseases, inhibition of iNOS overexpression is crucial. The production of NO is closely associated with alterations in the expression levels of its synthesizing enzyme, iNOS [ 40 , 47 ]. Researchers have shown that various probiotic Bifidobacterium , Lactobacillus , and Weissella strains inhibit NO production in RAW 264.7 cells or murine bone marrow-derived macrophages (BMDMs) [ 23 , 40 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory potential via the MAPK signaling pathway of Lactobacillus spp. isolated from canine feces

Park,

Jang

et al. 2024

PLoS ONE

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Two probiotic candidates, Lactobacillus reuteri C1 (C1) and Lactobacillus acidophilus C5 (C5), which were previously isolated from canines, were evaluated in the present study. L. reuteri and L. acidophilus have anti-oxidant, anti-inflammatory, immune-enhancing, and anti-cancer properties and exhibit various probiotic effects in humans and animals. The strains C1 and C5 demonstrated good tolerance to acid and bile salt exposure, exhibited effective adhesion to HT-29 cell monolayer, and displayed sensitivity to antibiotics, thus affirming their probiotic characteristics. Moreover, C1 and C5 exhibited the ability to downregulate the expression of inducible NO synthase (iNOS), an immunomodulatory factor, leading to a reduction in NO production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. These strains also demonstrated potent anti-inflammatory effects in LPS-stimulated RAW 264.7 cells, achieved through the augmentation of anti-inflammatory cytokine IL-10 expression and the inhibition of pro-inflammatory cytokine IL-1β expression. These anti-inflammatory effects of C1 and C5 were closely associated with the mitogen-activated protein kinase (MAPK) signaling pathway. The results of the present study suggest that the C1 and C5 probiotic candidates attenuate LPS-induced inflammation via the MAPK signaling pathway and the strains can be used as probiotics considering their anti-inflammatory potential.

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Section: Discussionmentioning

confidence: 99%

Anti-inflammatory potential via the MAPK signaling pathway of Lactobacillus spp. isolated from canine feces

Park,

Jang

et al. 2024

PLoS ONE

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“…Further studies by inhibiting the mechanism of bacterial-host cell interactions will help provide new ideas for drug targeting actions. In recent years, more and more studies have confirmed that edible probiotics and their metabolites are widely used for food preservation and regulation of human health and immunity (Pato et al, 2022;Shi et al, 2022). Therefore, in the food industry, it is more highlighted that the selection and breeding of high-yielding PLA strains, metabolic regulation, and the mechanism of their inhibition of spoilage and pathogenic bacteria still need more in-depth research, so that the application of PLA is more extensive.…”

Section: Discussionmentioning

confidence: 99%

A new anti-colon cancer tumor pathway of Phenyllactic acid by reducing adhesion of Fusobacterium nucleatum

WANG

et al. 2023

Food Sci. Technol

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The incidence of colorectal cancer (CRC) is increasing, with 1,931,590 new colorectal cancer patients worldwide in 2020. Fusobacterium nucleatum (Fn) has been widely reported as a harmful microorganism that promotes the development of CRC. Fn was found to enhance proliferation and metastasis of CRC cells by regulating cellular glucose metabolism and amino acid metabolism in trials. In a previous study, phenyl lactic acid (PLA), a natural metabolite of various probiotic bacteria, was found to have the ability to inhibit Fn as an organic acid. Therefore, further in-depth analysis of the mechanism of action of PLA in weakening Fn invasion into tumor cells provides a new therapeutic avenue for exacerbating CRC problems caused by intestinal pathogenic bacteria.

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“…It is important to point out that the Lactobacillaceae family includes many genera such as Lactobacillus , Paralactobacillus , and Pediococcus. Zheng et al ( 112 ) showed extensive diversity of the genus Lactobacillus , an integral part of the microbiome with anti-inflammatory action ( 113 ). Some studies associated decrease in Lactobacillus spp.…”

Section: Discussionmentioning

confidence: 99%

Effects of physical distancing by COVID-19 pandemic on diet quality, neurological and immunological markers, and fecal microbiota of Brazilian older women

et al. 2022

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Physical distancing was used to prevent transmission of COVID-19, however there are concerns that this may promote harmful impacts on health, such as reduced levels of physical practice and changes in food intake and gut microbiota composition. This study evaluated the impacts of 6 months physical distancing on Brazilian older women upon body mass index (BMI), strength, physical activity level (IPAQ), eating habits, neurological markers (brain-derived neurotrophic factor-BDNF and cortisol), cytokines (IL-2, IL-5, IL-6, IL-10, interferon-IFN-γ, tumor necrosis factor-TNF-α), aging-associated markers (vascular endothelial growth factor-VEGF, insulin-like growth factor-IGF-1, klotho and thymic stromal lymphopoietin-TSLP), besides specific groups of fecal microbiota. Fifteen women, over 60 years old, residents of São Paulo state (Brazil), were evaluated in March and in September 2020. The older adult women, with a mean age 66 ± 6.2 years presented significantly increased BMI and high effect size for non-protective foods consumption, reduced light physical activity and strength 6 months following the physical distancing. Furthermore, the serum concentration of IFN-γ, IGF-1, and IFN-γ/IL-5 were significantly higher, while lower concentration of IL-2 and IL-5 were observed 6 months after the physical distancing. Significant increase was noted only to Blautia spp. abundance after 6 months of physical distancing. Several correlations were observed at both before and after physical distancing, however, interestingly, many of them were lost or inverted 6 months following, while new ones emerged. Taken together, these results showed that lifestyle changes and stress conditions addressed by physical distancing from the COVID-19 pandemic impacted the health of older women included in the present study. Therefore, future follow-up studies are essential to propose interventions in order to restore the health conditions observed before the pandemic period, and thus to maintain the quality of life of older adults in different socioeconomic contexts.

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Selected lactobacilli strains inhibit inflammation in LPS-induced RAW264.7 macrophages by suppressing the TLR4-mediated NF-κB and MAPKs activation

Cited by 13 publications

References 36 publications

Anti-inflammatory potential via the MAPK signaling pathway of Lactobacillus spp. isolated from canine feces

Anti-inflammatory potential via the MAPK signaling pathway of Lactobacillus spp. isolated from canine feces

A new anti-colon cancer tumor pathway of Phenyllactic acid by reducing adhesion of Fusobacterium nucleatum

Effects of physical distancing by COVID-19 pandemic on diet quality, neurological and immunological markers, and fecal microbiota of Brazilian older women

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