Selected di- and tetranucleotide microsatellites from chromosomes 7, 12, 14, and Y in various Eurasian populations

Abstract

Microsatellite polymorphisms of nine Eurasian populations (> 1200 chromosomes) were analyzed for the following loci: i) intronic (gt)n stretches of three T cell receptor (TCR) B loci on chromosome 7 (TCRBV6S1, TCRBV6S3, TCRBV6S7); ii) an intergenic (gt)n repeat in the region between the TCRDV3 and TCRAJ61 elements on chromosome 14; iii) two tetranucleotide simple repeats (D12S66, D12S67), not linked to known genes on chromosome 12; iv) a Y-chromosomal (gata)n polymorphism (DYS19). In general, allele frequencies and heterozygosity rates were similar, but specific alleles were missing in one or more populations. Distinct DYS19 alleles predominated in particular cohorts. Different allele frequencies were observed for the TCR loci in European and Asian populations. Tetranucleotide polymorphisms were distributed normally, whereas TCR alleles displayed bimodal frequency profiles. For TCRBV6S1 and TCRBV6S7, this profile reflects a diallelic protein polymorphism that correlates exactly with the length of the intronic repeats.

MtDNA and Y chromosome polymorphisms in Hungary: inferences from the palaeolithic, neolithic and Uralic influences on the modern Hungarian gene pool

“…The most important feature is the very high incidence of the YCAII a5-b1 haplotype (69.8%). This frequency is one of the highest observed in Europe, 14,25,39,41,43,44 and is close to the value (78%) displayed by the Basques. 45,46 .…”

Long Y-associated (GATA)n alleles were observed in a few ethnic groups in Asia

“…Among Caucasians in both Germany and Brazil (Gomolka et al, 1994;Santos et al, 1993), 190bp allele was most frequently observed. While in Middle East and South Asia, the predominant alleles were 194 bp in Pakistani, Hindu, Thais and 198 bp in Kampuchea (Gomolka et al, 1994).…”

“…Among Caucasians in both Germany and Brazil (Gomolka et al, 1994;Santos et al, 1993), 190bp allele was most frequently observed. While in Middle East and South Asia, the predominant alleles were 194 bp in Pakistani, Hindu, Thais and 198 bp in Kampuchea (Gomolka et al, 1994). In another survey of alleles at the DYS19 locus, higher frequency of longer alleles was found in Japanese populations as compared to that of Western European populations (Hammer and Horai, 1995).…”

“…A polymorphism of a (GATA)n tetranucleotide-repeat type has been identified (Arnemann et aL, 1986) and this polymorphism can be examined by polymerase chain reaction (PCR) (Roewer et aL, 1992). The allele distributions of this locus have been reported in various populations (Santos et aL, 1993;Gomolka et aL, 1994). In this report, we studied the polymorphism of this locus in five ethnic groups and compared to previous reports.…”

Polymorphism of the D12s66 System in the Japanese Population and Its Detection Using Degraded Dna

“…The most predominant allele was allele 11. Although Gomolka et al 3) have examined the D12S66 locus in Germans and THE D12S66 SYSTEM IN JAPANESE Fig. 1 Electrophoresis of D12S66 in a 6% denaturing gel followed by silver staining.…”