Selectable and Screenable Markers for Rice Transformation

Twyman, Richard M.; Stöger, Eva; Kohli, Ajay; Capell, Teresa; Christou, Paul

doi:10.1007/978-3-662-04904-4_1

Cited by 17 publications

(11 citation statements)

References 71 publications

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“…They also demonstrated that the sensitivity of plant cells to the selection agent depends upon the genotype, the explant type and the developmental stage. Most of studies have reported that hygromycin can be used as a more efficient selectable marker, compared to kanamycin, and that it is an effective agent in plant transformation, very efficient in terms of regeneration, does not inhibit regeneration and does not affect the subsequent fertility of transgenic plants (Reynaerts et al 1988;Twyman et al 2002;Htwe et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and expression of a vacuolar Na+/H+ antiporter gene (AgNHX1) in fig (Ficus carica L.) under salt stress

Metwali

Soliman

Fuller

et al. 2015

Plant Cell Tiss Organ Cult

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Soil salinity can be a major limiting factor for productivity in agriculture and forestry and in order to fully utilize saline lands productively in plantation forestry for fig production, the genetic modification of tree species for salt tolerance may be required. Na ? /H ? antiporters have been suggested to play important roles in salt tolerance in plants.Here, we isolated AgNHX1 a vacuolar Na ? /H ? antiporter from a halophytic species Atriplex gmelini and introduced it into fig (Ficus carica L.) cv. Black Mission via Agrobacterium-mediated transformation. Leaf discs explants of fig were co-cultivated for 2 days with Agrobacterium tumefaciens strain LBA 4404 harboring the binary vector pBI121 containing the AgNHX1 gene and the hpt selectable marker gene which encodes hygromycin phosphotransferase. Explants were cultured on MS medium containing 30 mg L -1 hygromycin, 3 % sucrose, 0.2 mg L -1 kinetin and 2.0 mg L -1 2,4-dichlorophenoxyacetic acid solidified with 2.5 g L -1 phytagel in darkness for callus formation. The calli were cultured on MS medium containing 2.0 mg L -1 zeatin riboside in combination with 0.4 mg L -1 indole acetic acid in the light for plant regeneration. Putative regenerated transformant shoots were confirmed by polymerase chain reaction (PCR) and Southern hybridization for the AgNHX1 gene. Reverse transcriptase polymerase chain reaction analysis indicated that the gene was highly expressed in transgenic plants, but the degree of this expression varied among transformants. Overexpression of the AgNHX1 gene conferred high tolerance to salt stress and transgenic fig plants overexpressing AgNHX1 developed normally under salinity conditions compared to those of non-transgenic plants. Salt treated transgenic plants contained high proline and K ? but less Na ? compared to non-transgenic control plants.

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Section: Discussionmentioning

confidence: 99%

Molecular cloning and expression of a vacuolar Na+/H+ antiporter gene (AgNHX1) in fig (Ficus carica L.) under salt stress

Metwali

Soliman

Fuller

et al. 2015

Plant Cell Tiss Organ Cult

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“…Due to the hygromycin which can be applied at lower level than kanamycin, hygromycin appears an appropriate selective agent [66]. Similar results reported that hygromycin is an effective selective marker for genetic transformation for monocot plants such as rice [67,68], maize [69], and banana [70].…”

Section: Sugarcane -Technology and Researchmentioning

confidence: 70%

Biotechnology of Drought-Tolerant Sugarcane

Sugiharto¹

2018

Sugarcane - Technology and Research

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Water stress exists in most sugarcane cultivation areas, which are not supported by irrigation system and have low rain precipitation. Better understanding of physiological and biochemical mechanism, underlying plants response to water stress, have been achieved to develop drought-tolerant plants by biotechnology approach. To survive and grow normally, plants use a range of strategies to cope the water stress such as changes in gene expression and accumulation of organic compound called compatible solutes. Observation of drought stress response in sugarcane found the presence of a droughtinducible protein called SoDip22 and that the expression was induced by drought stress and ABA hormone treatments. However, the function of this drought-inducible protein has not been elucidated and only suggested that the protein may play an important role in maintenance of water molecule during water deficit state. Biochemical studies on the drought-tolerance mechanism have shown that nontoxic small compound of compatible solute accumulated during water deficit condition. Genetic engineering of glycine betaine (GB), acting as a compatible solute, has been applied for enhancement of water stress tolerance. In sugarcane, bacterial betA gene encodes for choline dehydrogenase (CDH) has successfully introduced and resulted in the transgenic drought-tolerance sugarcane. The CDH converts choline into betaine aldehyde, which is then converted to GB. The overexpression of betA gene increased GB contents that act as an osmoprotectant and help sugarcane acclimate in water deficit condition. This chapter reports the development of biotechnology for drought-tolerant sugarcane.

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“…It is commonly found that some selection systems are more effective in certain plant species and regeneration systems than others. An example is the lower efficiency of kanamycin resistance as a selection system in cereals than in dicots (Twyman et al 2002). E. pallida has lower shoot regeneration frequency with hygromycin (Table 2), and transgenic lines OSAG78-4 and OSAG78-6 had retarded shoot regeneration.…”

Section: Agrobacterium-mediated Transformationmentioning

confidence: 99%