Seleção de acessos e progênies de Citrullus spp. para resistência a três potyvirus

Silveira, Lindomar María da; Queiróz, M. A. de; Lima, José Albérsio de Araújo; Negreiros, Maria Zuleide de; Ramos, Najara F.; Nascimento, Aline Kelly Queiroz do

doi:10.1590/s0100-41582005000400009

Cited by 5 publications

(8 citation statements)

References 6 publications

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“…The viruses used in this study belong to the active bank of plant viruses from the Plant Virus Laboratory at the Federal University of Ceará (UFC), Fortaleza-CE, Brazil. ELISA plates trapped antigens were prepared with six virus species from the family Secoviridae, Comovirus genus (Squash mosaic virus, S Q M V a n d Cowpea severe mosaic virus, CPSMV), family Bromoviridae, Cucumovirus genus (Cucumber mosaic virus, CMV), family Potyviridae, Potyvirus genus (Cowpea aphidborne mosaic virus, CABMV and Zucchini yellow mosaic virus, ZYMV) and Sobemovirus (Papaya lethal yellowing virus, PLYV) in infected plant tissues (NASCIMENTO et al, 2010;SILVEIRA et al, 2005). ELISA plate wells were also treated with extracts from healthy plants of cowpea [Vigna unguiculata (L.) Walp subsp.…”

Section: Methodsmentioning

confidence: 99%

“…For this reason, biological, serological and molecular methods have been developed for identification and detection of plant virus infections in the field. The serological techniques have been the most used methods for rapid and efficient detection of plant viruses in several plant samples, including in programs to produce virus free plant material (LIMA et al, 2012;NAIDU;HUGHES, 2003;SHIM et al, 2006;UEHARA-ICHIKI et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…The indirect ELISA requires antibodies produced in two different animal species and the virus particles are trapped in the wells of the ELISA plate. For this reason, this method is also known as plate-trapped antigen (PTA) ELISA (LIMA et al, 2012;WARD et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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A Simple Kit of Plate-Trapped Antigen Enzyme-Linked Immunosorbent Assay for Identification of Plant Viruses

Nascimento

Lima

Barbosa

2017

Revista Ciência Agronômica

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Many methods can be used for arriving in a correct virus disease diagnose, and the serological techniques are the most used methods for plant virus identification. The indirect enzyme-linked immunosorbent assay (Indirect-ELISA) or plate-trapped antigen ELISA (PTA-ELISA) has been useful for detection of viruses in a wide range of situations, especially to test a large number of samples in a relatively short period of time. Immune-biological Companies have developed practical kits for direct ELISA or double antibody sandwich (DAS-ELISA), but neither Company has developed kits for PTA-ELISA. As a single universal antibody-conjugate is used for detection of a wide range of plant viruses, the PTA-ELISA technique is more economical, practical and suitable for virus detection. Considering also the great problem of including infectious plant viruses in DAS-ELISA kits, a simple kit for PTA-ELISA was developed for plant virus identification. Extracts from infected plant tissues were added into the ELISA plate wells, which were sealed with plastics and maintained in the refrigerator and Laboratory conditions for different periods of time. The plates were tested by the regular PTA-ELISA and after 20 months of incubation, the plate showed excellent results when used for detection of six virus species from the genera Comovirus, Cucumovirus, Potyvirus and Sobemovirus in infected plant tissues. The ELISA plate trapped virus together with its specific antiserum could constitute a simple kit, which will permit the exchange of antisera among virologists without transferring infectious viruses from one laboratory to another to be used as control.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Simple Kit of Plate-Trapped Antigen Enzyme-Linked Immunosorbent Assay for Identification of Plant Viruses

Nascimento

Lima

Barbosa

2017

Revista Ciência Agronômica

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“…lanatus, with a total of 2,079,547 tons produced (FAOSTAT, 2014). However, despite this high production, infections from fungi, bacteria, viruses, and nematodes cause plant diseases and biotic stress that reduce both productivity and fruit quality (SANTOS et al, 2005;SILVEIRA et al, 2005). Among these pathogens, plant-parasitic nematodes (phytonematodes that infest the root system of the watermelon plants) of the genus Meloidogyne not only cause irreparable losses in watermelon fields, but also it may create environmental damage if nematocides are applied.…”

Section: Introductionmentioning

confidence: 99%

EVALUATION OF THE REACTION OF WATERMELON PARENT AND F 1 PLANTS TO Meloidogyne enterolobii

et al. 2016

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-The aim of this study was to evaluate the performance of progenies from Citrullus lanatus var. lanatus (cultivated watermelons) when crossed with progenies from C. lanatus var. citroides (fodder watermelon with a historic of resistance to the nematode Meloidogyne enterolobii). The parents and their F 1s were evaluated for resistance to this nematode. In the initial stages of eleven treatments, watermelon seedlings plantlets were transplanted to plastic bags of six kilograms once the first leaves developed. Ten inoculated plants with 5,200 eggs in the soil near the stem of the plant and four non-inoculated ones were used in each treatment, in a complete block design. Sixty-two days after sowing, the following characteristics were evaluated: the length of the aerial part of the plant (LAP, in m), fresh mass of the aerial part (FMAP, in g), root fresh mass (RFM, in g), egg number (EN) and reproduction factor (RF). A comparison between the averages of inoculated and non-inoculated plants was performed using Scott-Knott test at 5% and the diallelic analysis was performed using the GENES program. The morphological characteristics did not allow for the identification of the parent plants or the F 1s with respect to nematode resistance, but the variables EN and RF were useful for such identification. The analyses of the general and specific combining abilities indicate highly significant effects with respect to this resistance, showing additive gene effects as well as dominance and epistatic gene effects, allowing for identification of parents and F 1s that can be used in watermelon breeding programs to improve resistance to the M. enterolobii.Keywords: Citrullus spp. Root-knot nematode. Watermelon breeding. AVALIAÇÃO DE PARENTAIS E F 1S EM MELANCIA QUANTO À REAÇÃO AO Meloidogyne enterolobii RESUMO -O objetivo deste estudo foi avaliar o desempenho de progênies de melancia provenientes deCitrullus lanatus var. lanatus (melancias cultivadas) quando cruzadas com progênies provenientes de C. lanatus var. citroides (melancias forrageiras com histórico de resistência ao nematoide Meloidogyne enterolobii). Os parentais e seus F 1s foram avaliados quanto à reação a esse nematoide. Mudas de melancia de onze tratamentos no estádio inicial, com a primeira folha definitiva recém desenvolvida foram plantadas em sacos plásticos de seis quilogramas. Utilizou-se dez plantas inoculadas com 5.200 ovos próximo ao colo da planta e quatro não inoculadas em cada tratamento em um delineamento inteiramente casualizado. Após 62 dias foram avaliados os seguintes caracteres: comprimento da parte aérea (CPA, em m), massa fresca da parte aérea (MFPA, em g), massa fresca de raiz (MFR, em g), número de ovos (NO) e o fator de reprodução (FR). A comparação das médias das plantas inoculadas e não inoculadas foi feita usando o teste de Scott-Knott ao nível de 5%. A análise dialélica foi realizada utilizando-se o programa GENES. Os caracteres morfológicos não permitiram identificar as plantas dos parentais e dos F 1s quanto à reação ao nematóide...

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“…Entretanto, atualmente não existem variedades de melancia resistentes a vírus sendo comercializadas, apesar da existência de fontes de melancia resistentes a potyvírus (OLIVEIRA et al, 2002;SILVEIRA et al, 2005).…”

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Seleção de linhagens de melancia resistentes ao Watermelon mosaic virus e ao Papaya ringspot virus

Júnior¹,

Figueira²,

Maluf³

2007

Ciênc. agrotec.

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RESUMOForam avaliadas 20 linhagens de melancia, provenientes do cruzamento da cultivar comercial suscetível Crimson Sweet e da introdução PI 595201 resistente ao Watermelon mosaic virus (WMV) e Papaya ringspot virus (PRSV-W). As linhagens, e os parentais foram inoculados com o WMV ou com o PRSV-W em casa-de-vegetação distintas. Aos 35 e 49 dias após a primeira inoculação (DAI), as plantas foram avaliadas por meio de uma escala de notas, em que 1 (ausência de sintomas) a 5 (intenso mosaico e deformações foliares). Pelos resultados infere-se que, aos 35 DAI, as linhagens 1, 2 e 20 apresentaram resistência tanto para o WMV como para o PRSV-W, com médias de 1,95, 1,80 e 2,25 para o WMV, e de 2,50, 2,30 e 2,50 para o PRSV-W, respectivamente. As linhagens 5, 7 e 13 foram resistentes somente ao WMV e as plantas das linhagens 3, 10 e 18 para o PRSV-W. A reação das linhagens permaneceu em geral pouco alterada aos 49 DAI. A existência de linhagens resistentes somente ao WMV e somente ao PRSV-W, ao lado de linhagens resistentes a ambos os vírus, é indicativo de que as resistências ao WMV e ao PRSV-W não são controladas pelos mesmos genes. Termos para indexação:Controle genético, Cucurbitaceae, Potyvirus. ABSTRACTTwenty advanced watermelon breeding lines, derived from the cross between cv. Crimson Sweet (susceptible) and PI 595201 (resistant to WMV and PRSV-W), were screened for resistance to both potyviruses. The twenty lines, among with Crimson Sweet and PI 595201, were inoculated with either WMV or PRSV-W, in two different greenhouse trials. Plants were evaluated for symptoms 35 and 49 days after the first inoculation (DAI), using a scale from 1 (no symptoms) to 5 (severe mosaic and foliar distortion). Evaluations at 35 DAI indicated that lines 1, 2 and 20 had good levels of resistance to both WMV and PRSV-W, with ratings of 1,95, 1,80 and 2,25 for WMV, and of 2,50, 2,30 and 2,50 for PRSV-W, respectively. Lines 5, 7 and 13 were resistant to WMV only, whereas lines 3, 10 and 18 were resistant to PRSV-W only. The reaction of the lines 49 DAI remained essentially unchanged. The existence of lines with resistance to WMV only and to PRSV-W only, along with lines with resistance to both viruses, indicates that resistance to WMV and PRSV-W are under control of different genes.

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Seleção de acessos e progênies de Citrullus spp. para resistência a três potyvirus

Cited by 5 publications

References 6 publications

A Simple Kit of Plate-Trapped Antigen Enzyme-Linked Immunosorbent Assay for Identification of Plant Viruses

A Simple Kit of Plate-Trapped Antigen Enzyme-Linked Immunosorbent Assay for Identification of Plant Viruses

EVALUATION OF THE REACTION OF WATERMELON PARENT AND F 1 PLANTS TO Meloidogyne enterolobii

Seleção de linhagens de melancia resistentes ao Watermelon mosaic virus e ao Papaya ringspot virus

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