Selaginella uncinata flavonoids ameliorated ovalbumin-induced airway inflammation in a rat model of asthma

Yu, Bing; Cai, Wei; Zhang, Huan‐Huan; Zhong, Yu-Sen; Fang, Jie; Zhang, Wenyou; Li, Mo; Weng, Lu-Chen; Yu, Chunjing

doi:10.1016/j.jep.2016.11.049

Cited by 24 publications

(13 citation statements)

References 72 publications

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“…Similarly, the increased levels of IL-10 in OVA-induced mice were significantly decreased after treatment with Viwithan. These results were in agreement with the previous reports where some plant extracts alleviated airway inflammation by regulating the production of inflammatory cytokines [31,32]. Our results also showed that Viwithan significantly attenuated the infiltration of eosinophils and other inflammatory cells.…”

Section: Discussionsupporting

confidence: 94%

Viwithan: A Standardized Ashwagandha Extract Ameliorates Ovalbumininduced Airway-Inflammation and Oxidative Stress in Mouse Model

Hs¹,

Hv²,

Illuri³

et al. 2021

Asian J Pharm Clin Res

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Objective: Withania somnifera, commonly known as Ashwagandha, Indian ginseng, has been used in Ayurvedic and indigenous medicinal preparations for various disease conditions since long time. In the present study, we investigated the protective effects of Viwithan, a standardized proprietary extract from Ashwagandha roots, against airway-inflammation and oxidative stress modulation in an ovalbumin (OVA)-induced murine model of inflammation. Methods: Allergic asthma was initiated in BALB/c mice by sensitizing with OVA on days 1 and 14, followed by intranasal challenge with OVA on days 27, 28, and 29. Mice were administered Viwithan (200 and 400 mg/kg) by oral gavage before challenge. Then, mice were evaluated for the presence of airway inflammation, production of allergen-specific cytokine response, lung pathology, and oxidative stress modulation. Results: The results showed that treatment with Viwithan attenuated OVA-induced lung inflammation in mice. Viwithan significantly attenuated inflammatory cell infiltration into the bronchoalveolar lavage fluid and markedly reduced the levels of pro-inflammatory cytokines, interleukin-10, and transforming growth factor-β1 in lung tissues. Viwithan treatment considerably reduced the lung weight in OVA-sensitized mice. Viwithan markedly attenuated the OVA-induced generation of reactive oxygen species in lung tissues. Conclusion: Together, these results suggested that Viwithan alleviates OVA-induced airway-inflammation and oxidative stress, highlighting the potential of standardized Ashwagandha extract as a useful therapeutic agent for pulmonary fibrosis management.

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Section: Discussionsupporting

confidence: 94%

Viwithan: A Standardized Ashwagandha Extract Ameliorates Ovalbumininduced Airway-Inflammation and Oxidative Stress in Mouse Model

Hs¹,

Hv²,

Illuri³

et al. 2021

Asian J Pharm Clin Res

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“…Bitter compounds and their agonists, including quinine, quinidine, chloroquine, and ally isothiocyanate induced apoptosis and suppressed cell proliferation in breast cancer and bladder cancer [25, 62]. Furthermore, analysis of a glioma genome-wide association dataset identified a possible contribution by a TAS2R8 single nucleotide polymorphism to glioma susceptibility [63], while TAS2R10 has been found to be up-regulated in patients with Parkinson disease and to have a role in the treatment of asthma in rats [64, 65]. These results indicate possible physiologic functions of TAS2Rs, although it has not been confirmed whether the functions of TAS2R8 and TAS2R10 contribute to cancers.…”

Section: Discussionmentioning

confidence: 99%

Anti-cancer stemness and anti-invasive activity of bitter taste receptors, TAS2R8 and TAS2R10, in human neuroblastoma cells

et al. 2017

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Neuroblastoma (NB) originates from immature neuronal cells and currently has a poor clinical outcome. NB cells possess cancer stem cells (CSCs) characteristics that facilitate the initiation of a tumor, as well as its metastasis. Human bitter taste receptors, referred to as TAS2Rs, are one of five types of basic taste receptors and they belong to a family of G-protein coupled receptors. The recent finding that taste receptors are expressed in non-gustatory tissues suggest that they mediate additional functions distinct from taste perception. While it is generally admitted that the recognition of bitter tastes may be associated with a self-defense system to prevent the ingestion of poisonous food compounds, this recognition may also serve as a disease-related function in the human body. In particular, the anti-cancer stemness and invasion effects of TAS2Rs on NB cells remain poorly understood. In the present study, endogenous expression of TAS2R8 and TAS2R10 in SK-N-BE(2)C and SH-SY5Y cells was examined. In addition, higher levels of TAS2R8 and TAS2R10 expression were investigated in more differentiated SY5Y cells. Both TAS2Rs were up-regulated following the induction of neuronal cell differentiation by retinoic acid. In addition, ectopic transfection of the two TAS2Rs induced neurite elongation in the BE(2)C cells, and down-regulated CSCs markers (including DLK1, CD133, Notch1, and Sox2), and suppressed self-renewal characteristics. In particular, TAS2RS inhibited tumorigenicity. Furthermore, when TAS2Rs was over-expressed, cell migration, cell invasion, and matrix metalloproteinases activity were inhibited. Expression levels of hypoxia-inducible factor-1α, a well-known regulator of tumor metastasis, as well as its downstream targets, vascular endothelial growth factor and glucose transporter-1, were also suppressed by TAS2Rs. Taken together, these novel findings suggest that TAS2Rs targets CSCs by suppressing cancer stemness characteristics and NB cell invasion, thereby highlighting the chemotherapeutic potential of bitter taste receptors.

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“…These findings provide important evidence that naringin and artesunate may be bronchodilators for treating asthma. Yu et al (2017) investigated the protective mechanisms of bitter total flavonoids from Selaginella uncinata on airway hyperresponsiveness in a rat model of OVA-treated asthma. They demonstrated that total flavonoids exerted anti-inflammatory function through the activation of TAS2R10.…”

Section: Bitter Components Extracted From Bitter Flavored Tcmmentioning

confidence: 99%

Toward the Identification of Extra-Oral TAS2R Agonists as Drug Agents for Muscle Relaxation Therapies via Bioinformatics-Aided Screening of Bitter Compounds in Traditional Chinese Medicine

Luo

Jin

et al. 2019

Front. Physiol.

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Significant advances have been made in the past decade in mapping the distributions and the physiological functions of extra-oral bitter taste receptors (TAS2Rs) in nongustatory tissues. In particular, it has been found that TAS2Rs are expressed in various muscle tissues and activation of TAS2Rs can lead to muscle cell relaxation, which suggests that TAS2Rs may be important new targets in muscle relaxation therapy for various muscle-related diseases. So far, however, there is a lack of potent extra-oral TAS2R agonists that can be used as novel drug agents in muscle relaxation therapies. Interestingly, traditional Chinese medicine (TCM) often characterizes a drug's property in terms of five distinct flavors (bitter, sweet, sour, salty, and pungent) according to its taste and function, and commonly regards "bitterness" as an intrinsic property of "good medicine." In addition, many bitter flavored TCM are known in practice to cause muscle relaxation after long term use, and in lab experiments the compounds identified from some bitter flavored TCM do activate TAS2Rs and thus relax muscle cells. Therefore, it is highly possible to discover very useful extra-oral TAS2R agonists for muscle relaxation therapies among the abundant bitter compounds used in bitter flavored TCM. With this perspective, we reviewed in literature the distribution of TAS2Rs in different muscle systems with a focus on the map of bitter flavored TCM which can regulate muscle contractility and related functional chemical components. We also reviewed the recently established databases of TCM chemical components and the bioinformatics software which can be used for high-throughput screening and data mining of the chemical components associated with bitter flavored TCM. All together, we aim to present a knowledge-based approach and technological platform for identification or discovery of extra-oral TAS2R agonists that can be used as novel drug agents for muscle relaxation therapies through screening and evaluation of chemical compounds used in bitter flavored TCM.

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Selaginella uncinata flavonoids ameliorated ovalbumin-induced airway inflammation in a rat model of asthma

Cited by 24 publications

References 72 publications

Viwithan: A Standardized Ashwagandha Extract Ameliorates Ovalbumininduced Airway-Inflammation and Oxidative Stress in Mouse Model

Viwithan: A Standardized Ashwagandha Extract Ameliorates Ovalbumininduced Airway-Inflammation and Oxidative Stress in Mouse Model

Anti-cancer stemness and anti-invasive activity of bitter taste receptors, TAS2R8 and TAS2R10, in human neuroblastoma cells

Toward the Identification of Extra-Oral TAS2R Agonists as Drug Agents for Muscle Relaxation Therapies via Bioinformatics-Aided Screening of Bitter Compounds in Traditional Chinese Medicine

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