Segregation of Transcription and Replication Sites Into Higher Order Domains

Wei, Xiangyun; Samarabandu, Jagath; Devdhar, Rakendu S.; Siegel, Alan J.; Acharya, Raj; Berezney, Ronald

doi:10.1126/science.281.5382.1502

Cited by 240 publications

(215 citation statements)

References 16 publications

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“…Altogether, these data suggest that replication and transcription could be functionally and temporally coupled. However, other studies in the nucleus mammalian cells show that replication and transcription sites are separate, although interdigitated (Wei et al, 1998). Considering these findings together with the role of BCL6 in transcriptional control, we also directly address the possible role of BCL6 bodies in RNA synthesis and/or storage.…”

Section: Discussionmentioning

confidence: 99%

“…These data suggested that BCL6 bodies are associated with replicating DNA and/or that their close vicinity is a nuclear micro-environment prone to support DNA replication, and raised the possibility that they influence the replication foci (RF) assembly, positioning, or activity (Albagli et al, 2000). Moreover, together with the proposed spatial coincidence or close apposition between transcription and replication sites in nucleus (Fakan and Nobis, 1978;Jaunin et al, 1998Jaunin et al, , 2000Wei et al, 1998), these previous data led us here to also compare the localization of BCL6 bodies and RNA.…”

Section: Introductionmentioning

confidence: 97%

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The relationship between BCL6 bodies and nuclear sites of normal and halogenated DNA and RNA synthesis

Puvion‐Dutilleul¹,

Souquere-Besse²,

Albagli-Curiel

2003

Microscopy Res & Technique

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BCL6 is a POZ/BTB and zinc finger transcription factor that self-interacts and accumulates into discrete nuclear "bodies" of unknown function. We recently reported that BCL6 bodies associate with bromodeoxyuridine (BrdU)-substituted DNA, suggesting their implication in replication. To examine this possibility, we examine here by electron and confocal microscopy the relation between BCL6 bodies and replication foci (RF) using incorporation of various halogenated nucleotides (BrdU, chlorodeoxyuridine, CldU, and iododeoxyuridine, IdU) or PCNA (proliferating cell nuclear antigen) staining. We show that BCL6 bodies are found associated with RF, as revealed by PCNA staining. However, such association is markedly prolonged upon BrdU or CldU incorporation, but less, or not at all, upon IdU incorporation. Pulse-chase and double-labeling experiments indicate that IdU-substituted DNA leaves BCL6 bodies after a few tenths of minutes while BrdUor CldU-substituted DNA stalls in their vicinity for several hours, thereby giving the characteristic "crowns" of DNA entirely surrounding BCL6 bodies. In all cases, however, the halogenated DNA ends up undergoing a movement from BCL6 bodies toward nucleoplasm and nuclear periphery to reach euchromatin and heterochromatin, respectively. We propose that replicating DNA is prone to be bound by BCL6, while BrdU/CldU incorporation increases this propensity possibly because these two events have synergistic effects on the structure and chromatinisation of the newly synthesized DNA. Finally, despite the known proximity between nuclear sites of transcription and replication, we show via several approaches that BCL6 bodies do not appear to be involved either in RNA synthesis or storage.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

The relationship between BCL6 bodies and nuclear sites of normal and halogenated DNA and RNA synthesis

Puvion‐Dutilleul¹,

Souquere-Besse²,

Albagli-Curiel

2003

Microscopy Res & Technique

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“…The architecture of the cell nucleus exerts a regulatory role on a variety of functions, e.g., DNA replication, transcription, and RNA splicing (1)(2)(3)(4)(5). The eukaryotic genome is organized in large-scale chromatin domains occupying functionally distinct nuclear compartments (6,7).…”

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confidence: 99%

Spatial distribution patterns of interphase centromeres during retinoic acid‐induced differentiation of promyelocytic leukemia cells

et al. 2002

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Background:The pericentromeric heterochromatin is an important element for the regulation of gene silencing. Its spatial distribution during interphase appears to be celltype specific. This study analyzes three-dimensional (3D) centromere distribution patterns during cellular differentiation along the neutrophil pathway. Methods: Differentiation of the promyelocytic leukemia cell line NB4 was induced by retinoic acid. Centromeres in interphase nuclei were visualized by immunofluorescence staining of centromere-associated proteins with CREST serum. 3D images of nuclei were obtained by confocal microscopy. Automated methods for the segmentation of point-like objects in 3D images were implemented to detect the position of centromeres. Features of centromere localization patterns were determined by constructing the minimal spanning tree of the centromere distribution.

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“…This striking complexity is not surprising in view of the involvement of the nuclear matrix with many aspects of nuclear functioning. Recent evidence suggests that the matrix represents the architectural basis that mediates chromosome territory organization (3) and segregates numerous separate transcription and replication sites into still higher-order domains (4). Clearly, the nuclear matrix does not represent merely a distinct set of structural proteins.…”

mentioning

confidence: 99%

The Protein Composition of the Hepatocyte Nuclear Matrix Is Differentiation‐Stage Specific

Ivanović‐Matić¹,

Dinić²,

Vujošević³

et al. 2000

IUBMB Life

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SummaryThe protein composition of hepatocyte nuclear matrices was examined in rats from the 16th day of gestation to 75 days after birth (adult). An overall increase in size of the nuclear matrix was accompanied by quantitative and qualitative changes in its protein content. Quantitative changes of the major proteins of the peripheral lamina surrounding the isolated nuclear matrix were detected. By Western analysis we established that in pre-and postnatal nuclear matrices the relative concentrations of lamin C were greater than lamin A. After birth, the relative concentrations of both lamins progressively increased. In the adult nuclear matrix, the concentration of lamin A was greater than lamin C. In contrast, the relative concentrations of lamin B remained unchanged throughout development and growth. The relative concentrations of two nuclear matrix -associated regulatory proteins studied changed with development and growth: transcription factor C/EBP® isoforms, which were detected during the gestation period, increased notably after the rst postnatal day, attaining a maximum at the adult stage; the high concentrations of the proliferating cell nuclear antigen (PCNA) perceptibly decreased after the 21st prenatal day. Changes in the composition of the nuclear matrix protein suggest that this structure coordinates nuclear functioning during cell differentiation.IUBMB Life, 49: 511 -517, 2000 Keywords C/EBPa ; hepatocyte development; lamins; nuclear matrix; PCNA.The nuclear matrix is a proteinaceous structural framework of the nucleus. The isolated matrix has a tripartite structure and

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Segregation of Transcription and Replication Sites Into Higher Order Domains

Cited by 240 publications

References 16 publications

The relationship between BCL6 bodies and nuclear sites of normal and halogenated DNA and RNA synthesis

The relationship between BCL6 bodies and nuclear sites of normal and halogenated DNA and RNA synthesis

Spatial distribution patterns of interphase centromeres during retinoic acid‐induced differentiation of promyelocytic leukemia cells

The Protein Composition of the Hepatocyte Nuclear Matrix Is Differentiation‐Stage Specific

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