Segregation and Recombination in Inter-Gene Pool Crosses of Phaseolus Vulgaris L.

Paredes, Oscar M.; Gepts, Paul

doi:10.1093/oxfordjournals.jhered.a111556

Cited by 18 publications

(9 citation statements)

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“…Meanwhile, six Mesoamerican genotypes (D30, D509, D90064, D90066, D90062, and D90065) clustered in different subgroups within the Andean genepool and may also be the result of gene fl ow. Related to this, Paredes and Gepts (1995b) found a high level of preferential transmission of the Mesoamerican alleles over the Andean alleles in intergenepool crosses with Chilean genotypes. The use of Chilean germplasm as bridging germplasm has been suggested as a way to help reduce incompatibility in inter-genepool crosses or crosses with wild accessions (Paredes and Gepts, 1995a).…”

Section: Discussionmentioning

confidence: 73%

“…These results were confi rmed from a wider sample of common bean genotypes collected in Chile, using randomly amplifi ed polymorphic DNA (RAPD) markers (Vera et al, 1999). The genetic analysis by Paredes and Gepts (1995b) also detected rare polymorphisms for some allozyme data and a preferential transmission of the Mesoamerican alleles in crosses of Chilean genotypes with genotypes from diff erent races. During that study, Nei's genetic distance detected a high percentage of the total variation between landraces and only a small proportion within populations.…”

Section: Microsatellite Marker Characterization Of Chilean Common Beamentioning

confidence: 88%

“…During that study, Nei's genetic distance detected a high percentage of the total variation between landraces and only a small proportion within populations. The genetic analysis by Paredes and Gepts (1995b) also detected rare polymorphisms for some allozyme data and a preferential transmission of the Mesoamerican alleles in crosses of Chilean genotypes with genotypes from diff erent races. The high level of introgression in the Chilean germplasm detected in this study was not confi rmed by Johns et al, (1997) using RAPD markers and a diff erent set of accessions.…”

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confidence: 88%

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Microsatellite Marker Characterization of Chilean Common Bean (Phaseolus vulgaris L.) Germplasm

et al. 2010

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The Chilean national common bean germplasm collection consists of 1200 accessions, most of which have not been characterized or used in breeding programs. As part of the characterization of this germplasm we have formed a core collection of 246 accessions that represent 21% of the whole collection and that consists of many race Chile representatives as well as varieties from other races that are traditionally grown in the country. The specific objectives of this work were to use microsatellites to evaluate the level of polymorphism in the Chilean core collection and to determine the genetic variability of race Chile and its relationships with other races. The results indicate that the level of polymorphism detected with microsatellites was higher than that obtained with previous molecular or biochemical markers. The number of microsatellite alleles varied from 2 to 14 and the polymorphism information content (PIC) values were 0.08 to 0.84. Heterozygosity ranged from 0 to 0.052 for each of the markers. Euclidean genetic distance ranged from 0.12% between race Chile and race Nueva Granada to 0.71% between race Chile and race Mesoamerica. Multiple correspondence analysis (MCA) showed clustering of the accessions into the two main genepools, with race Chile genotypes grouping mainly with race Nueva Granada genotypes.

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Section: Discussionmentioning

confidence: 73%

Section: Microsatellite Marker Characterization Of Chilean Common Beamentioning

confidence: 88%

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confidence: 88%

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Microsatellite Marker Characterization of Chilean Common Bean (Phaseolus vulgaris L.) Germplasm

et al. 2010

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“…All of the distorted loci on linkage groups B5, B10a, and B10b had an excess of CO72548 alleles, indicating the presence of factors that inhibited transmission of G122 alleles on those chromosomes. Paredes and Gepts (1995) examined segregation distortions in Andean × Middle American gene pool crosses and also found that the distorted markers had an over‐representation of Middle American alleles. The large number of distorted markers on B10 is consistent with previous reports for marker segregation distortion in common bean (Blair et al, 2003; Fall et al, 2001).…”

Section: Resultsmentioning

confidence: 99%

Quantitative Trait Loci Linked to White Mold Resistance in Common Bean

et al. 2007

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White mold disease (WM) of common bean (Phaseolus vulgaris L.), caused by Sclerotinia sclerotiorum (Lib.) de Bary, reduces crop yield and quality throughout the United States. The development of cultivars with resistance to WM would be facilitated by the identification and use of molecular markers linked to resistance genes. The objectives of this research were (i) to characterize WM reaction in a recombinant inbred line (RIL) population derived from a cross between resistant and susceptible germplasm, (ii) to validate the effect of a previously reported quantitative trait locus (QTL) for WM resistance, and (iii) to locate additional QTL associated with WM resistance. A RIL population that consisted of 94 lines was derived from a cross between G122 (resistant) and CO72548 (susceptible). The population was evaluated for WM reaction in three greenhouse tests and one field environment, and for molecular markers throughout the genome. Two RIL were identified with higher resistance levels (P < 0.05) than the resistant parent G122. A previously reported QTL on linkage group B7 was significant (P < 0.01) in single‐factor analysis of variance, but not with composite interval mapping. Five QTL for resistance to WM were found (likelihood odds ratio [LOD] > 2.7) on linkage groups B1, B2b, B8, and B9. The QTL were contributed from both parents and together accounted for 48% of the phenotypic variation (R2). For field resistance, one QTL (R2 = 12%) on linkage group B8 was detected. These results confirm polygenic resistance to WM in common bean.

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“…Porém, este mapa de ligação ainda é limitado quanto a informações genéticas sobre características úteis para os melhoristas (resistência a doenças, por exemplo) e não é suficientemente saturado para os geneticistas interessados no isolamento de genes a partir de diversas técnicas de clonagem. Outra limitação refere-se ao fato de as distâncias genéticas variarem consideravelmente entre os mapas de ligação construídos a partir de cruzamentos entre diferentes cultivares de feijão PAREDES;GEPTS, 1995).…”

Section: Introductionunclassified

Desenvolvimento de marcadores SSR-EST e construção de mapas genéticos em feijão-comum (Phaseolus vulgaris L.)

Hanai¹

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Development of EST-SSR markers and construction of genetic maps in common bean (Phaseolus vulgaris)The use of molecular markers has contributed to the studies regarding domestication of Phaseolus species, origin and diversity of current common bean cultivars (P. vulgaris), and the genetic control of resistance to several diseases. Linkage maps have been constructed for common bean by using RFLP, RADP, SCAR, isoenzymes and phenotypic markers, some of which were meeting in a more dense and complete map of bean genome, called core map. However, for the effective use of linkage maps in breeding programs they must be sufficiently saturated. This work fits in this context, aiming to saturate the core map of P. vulgaris from the mapping of new molecular markers as AFLP and SSR EST-based. Thus, it was tried to develop and characterize SSR markers from a EST library, to test the transferability of these markers to other cultivars and related species, to generate AFLP markers and integrate them to the consensus map of the species. Also, build a genetic map for a population of interest in Brazil ( 'Carioca' x 'Flor de Mayo'), and promoting its alignment with the consensus map, thus complementing the genomic characterization of the species. Pairs of primers were designed for 156 EST-SSR. Of these, 138 EST-SSR amplified clear and reproducible loci and were characterized using a set of 26 genotypes of cultivated beans. Of the loci tested, 85 were polymorphic between the genotypes studied and showed an average 2.96 alleles per locus and a PIC average of 0.38. Among all examined EST-SSR, 50 loci segregated in 'Bat 93' x 'Jalo EEP558' mapping population, 20 loci segregated in 'Carioca' x 'Flor de Mayo' population and 12 loci were polymorphic in both two populations. AFLP markers were generated and genotyped in the two populations. The 262 microsatellites and AFLP loci genotyped in 'Bat 93' x 'Jalo EEP558' population were integrated onto the core map of the species. A map of 1353 cM total length was obtained, containing 357 markers, including 9 genomic-SSR, 47 EST-SSR and 190 AFLP. Moreover, another map was generated from the analysis of segregation of 252 markers in 'Carioca' x 'Flor de Mayo' population. This map was 807.5 cM long, with an average distance of 5.3 cM between markers. The common microsatellites markers were used as a bridge to align and compare the maps of the two studied populations.

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Segregation and Recombination in Inter-Gene Pool Crosses of Phaseolus Vulgaris L.

Cited by 18 publications

References 40 publications

Microsatellite Marker Characterization of Chilean Common Bean (Phaseolus vulgaris L.) Germplasm

Microsatellite Marker Characterization of Chilean Common Bean (Phaseolus vulgaris L.) Germplasm

Quantitative Trait Loci Linked to White Mold Resistance in Common Bean

Desenvolvimento de marcadores SSR-EST e construção de mapas genéticos em feijão-comum (Phaseolus vulgaris L.)

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