Segregation and early dispersal of neural crest cells in the embryonic zebrafish

Raible, David W.; Wood, Andrew; Hodsdon, Wendy; Henion, Paul D.; Weston, James A.; Eisen, Judith S

doi:10.1002/aja.1001950104

Cited by 195 publications

(182 citation statements)

References 39 publications

(39 reference statements)

Supporting

Mentioning

174

Contrasting

Order By: Relevance

“…Doublelabeling with the zn-5 (not shown) and znp-1 mAbs allowed us to recognize both the muscle pioneers and the C aP growth cone. The C aP growth cone and axon were closely associated with the surface of the muscle pioneers, the basal lamina of the notochord, and other migratory cells that are probably neural crest cells (Raible et al, 1992). There were sites of close membrane apposition between muscle pioneers and C aP axons; intense antibody labeling of motor axons did not allow us to determine whether synaptic vesicles were present near regions of close apposition or to examine fine structural details.…”

Section: Growth Cones Of Primary Motoneurons Interact Specifically Wimentioning

confidence: 90%

“…C aP and MiP were intracellularly labeled to assess their outgrowth and cell-specific trajectories. Individual motoneurons were labeled by intracellular injection of lysinated rhodamine dextran or lysinated fluorescein dextran (3 ϫ 10 3 M r ) (Molecular Probes, Eugene, OR) as described previously (Raible et al, 1992). Embryos were mounted as described in Eisen et al (1989).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Pathfinding by Identified Zebrafish Motoneurons in the Absence of Muscle Pioneers

et al. 1997

Self Cite

View full text Add to dashboard Cite

To identify the cellular cues that guide zebrafish neuronal growth cones to their targets, we examined interactions between identified motor growth cones and identified muscle fibers and tested whether these fibers were required for growth cone navigation. Caudal primary motoneurons (CaPs) and middle primary motoneurons (MiPs) are identified motoneurons that innervate cell-specific regions of the myotome. Growth cones of both cells initially extend along a common pathway and then pause at a set of identified muscle fibers, called muscle pioneers, before diverging along cell-specific pathways. Muscle pioneers are intermediate targets of both CaP and MiP Liu and Westerfield, 1990); both motoneurons extend their growth cones directly to the muscle pioneers on which the first functional neuromuscular contacts form, suggesting that muscle pioneers may provide guidance information to these growth cones. We tested this idea by ablating muscle pioneers and observing the resulting motor axonal trajectories. Both CaP and MiP ultimately formed normal axonal arbors after muscle pioneer ablation, showing that muscle pioneers are unnecessary for formation of correct axonal trajectories; however, although final cellular morphology was correct in the absence of muscle pioneers, MiP growth cones branched abnormally or extended ventrally beyond the common pathway. Ablation of CaP and the muscle pioneers together increased the aberrant behavior of the MiP growth cone. Our results provide evidence that an intermediate target, the muscle pioneers, affects motor axonal extension without altering target choice, suggesting that other cues also contribute to proper pathway navigation. Key words: acetylcholine receptors; neuromuscular junctions; axogenesis; zebrafish motoneurons; muscle pioneers; pathway navigationThe environment through which growth cones navigate contains cues that regulate neuronal pathfinding (Frank and Wenner, 1993;Goodman and Shatz, 1993;Keynes and Cook, 1995). En route to their synaptic targets, neurons may project axons to intermediate targets that influence subsequent pathway choice . For example, some grasshopper pioneer neurons project to guidepost cells (Bentley and Keshishian, 1982) that are required for normal pathfinding (Bentley and Caudy, 1983). Chick hindlimb motoneurons (Lance-Jones and Landmesser, 1981; Tosney and Landmesser, 1985a,b;Landmesser, 1992) and spinal commissural neurons (Bovolenta and Dodd, 1991) also project to specific intermediate targets, which appear to influence extension. These results suggest that intermediate targets serve as choice points for pathway selection.We examined the role of potential intermediate targets in the pathway choices of zebrafish primary motoneurons. We focused on the first two primary motoneurons to extend growth cones out of the spinal cord: C aP and MiP (see Fig. 1) Myers et al., 1986). The C aP growth cone pioneers a common pathway to the nascent horizontal myoseptum, where it pauses before selecting its cell-specific pathway along ventral myotome Myers e...

show abstract

Section: Growth Cones Of Primary Motoneurons Interact Specifically Wimentioning

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Pathfinding by Identified Zebrafish Motoneurons in the Absence of Muscle Pioneers

et al. 1997

Self Cite

View full text Add to dashboard Cite

show abstract

“…6B,C). At the same stage, neural crest cells that generate DRG neurons are still migrating between the somites and neural tube (Raible et al, 1992). Antibody labeling with anti-Hu detects DRG neurons as early as 2d.…”

Section: Rb Cell Death Is Independent Of Drg Formationmentioning

confidence: 99%

“…Individual RBs and DRG neurons in the trunk were injected with a combination of lysinated rhodaminedextran and lysinated fluorescein dextran (3,000 M r , Molecular Probes) as described previously (Raible et al, 1992) and monitored by using a Zeiss CLSM. Thin optical sections encompassing labeled cells were captured, and final images were obtained by processing with NIH Image.…”

Section: Intracellular Dye-labelingmentioning

confidence: 99%

Slow degeneration of zebrafish Rohon‐Beard neurons during programmed cell death

Reyes

Haendel

Grant

et al. 2003

Developmental Dynamics

Self Cite

View full text Add to dashboard Cite

Rohon-Beard cells are large, mechanosensory neurons located in the dorsal spinal cord of anamniote vertebrates. In most species studied to date, these cells die during development. We followed labeled Rohon-Beard cells in living zebrafish embryos and found that they degenerate slowly, over many days. During degeneration, the soma shrinks and finally disappears, and the processes become beady in appearance and finally break apart, but they do not retract. Zebrafish Rohon-Beard cells apparently fragment their DNA, as revealed by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) labeling, before undergoing degenerative morphologic changes. We also followed the development of labeled dorsal root ganglion neurons, as they are developing at the same stages that Rohon-Beard cells are degenerating. We found that, although axons of both cell types extend into similar regions, Rohon-Beard cells degenerate normally in mutants lacking dorsal root ganglia, providing evidence that interactions between the two cell types are not responsible for Rohon-Beard cell degeneration. Developmental Dynamics 229:30 -41, 2004.

show abstract

“…This technique was applied in numerous zebra-fish cell fate studies (Collazo et al, 1994;Devoto et al, 1996;Raible et al, 1992). In the context of visual system development, iontophoretic cell labeling was used to determine the developmental origins of the optic primordium (Woo and Fraser, 1995) and later to study cell rearrangements that accompany optic cup morphogenesis (Li et al, 2000b).…”

Section: Analysis Of Cell Movements and Lineage Relationshipsmentioning

confidence: 99%