Segmented negative strand RNA virus nucleoprotein structure

Reguera, Juan; Cusack, Stephen; Kolakofsky, Daniel

doi:10.1016/j.coviro.2014.01.003

Cited by 39 publications

(35 citation statements)

References 47 publications

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“…The NP/N protein is the most abundant element in the NSV RNPs, it provides the basis for their helical structure and is essential for the transcription and replication of full-length templates (for reviews see Ivanov et al, 2011;Reguera et al, 2014;Ruigrok et al, 2010;Ruigrok et al, 2011). The NSV NP/N proteins show a general crescent form with two domains.…”

Section: The Nucleoproteinmentioning

confidence: 99%

“…Due to space limitations, many important contributions could not be directly cited. More detailed information is available in recent specific reviews (Albertini et al, 2011;Boivin et al, 2010;Eisfeld et al, 2014;Fodor, 2013;Ivanov et al, 2011;Kranzusch and Whelan, 2012;MartinBenito and Ortin, 2013;Morin et al, 2013;Reguera et al, 2014;Resa-Infante et al, 2011;Ruigrok et al, 2010;Ruigrok et al, 2011;.…”

Section: Introductionmentioning

confidence: 96%

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The RNA synthesis machinery of negative-stranded RNA viruses

Ortı́n

Martín‐Benito

2015

Virology

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The group of Negative-Stranded RNA Viruses (NSVs) includes many human pathogens, like the influenza, measles, mumps, respiratory syncytial or Ebola viruses, which produce frequent epidemics of disease and occasional, high mortality outbreaks by transmission from animal reservoirs. The genome of NSVs consists of one to several single-stranded, negative-polarity RNA molecules that are always assembled into mega Dalton-sized complexes by association to many nucleoprotein monomers. These RNA-protein complexes or ribonucleoproteins function as templates for transcription and replication by action of the viral RNA polymerase and accessory proteins. Here we review our knowledge on these large RNA-synthesis machines, including the structure of their components, the interactions among them and their enzymatic activities, and we discuss models showing how they perform the virus transcription and replication programmes.

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Section: The Nucleoproteinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

The RNA synthesis machinery of negative-stranded RNA viruses

Ortı́n

Martín‐Benito

2015

Virology

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“…In the viral context, the negative-stranded genome RNA is entirely encapsidated by viral nucleoprotein and the promoter covered by the polymerase complex [21,22]. Moreover, recently solved atomic structures revealed that the canonical ''panhandle'' is actually not formed when in the polymerase-associated state, since the 5 0 and 3 0 extremities are bound on separate sites [23,24].…”

Section: How Can Rig-i Access the Encapsidated Panhandle?mentioning

confidence: 98%

The catcher in the RIG-I

Weber

2015

Cytokine

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“…The protein N thus has an important role in the protection of the viral genetic information. In the last 5 years, the crystal structure of N has been solved for many bunyavirus members, providing new insights into the mechanism of RNP assembly [69][70][71][72][73][74][75][76][77][78][79][80][81]. Briefly, the protein N of orthobunyaviruses binds to RNA in a positively charged cleft, formed by two-helical lobes [69][70][71][72][73]75].…”

Section: Virion Structurementioning

confidence: 99%

Bunyaviruses: From Transmission by Arthropods to Virus Entry into the Mammalian Host First-Target Cells

Léger

Lozach

2015

Future Virol.

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The Bunyaviridae constitute a large family of animal RNA viruses distributed worldwide, most members of which are transmitted to vertebrate hosts by arthropods and can cause severe pathologies in humans and livestock. With an increasing number of outbreaks, arthropod-borne bunyaviruses (arbo-bunyaviruses) present a global threat to public health and agricultural productivity. Yet transmission, tropism, receptors and cell entry remain poorly characterized. The focus of this review is on the initial infection of mammalian hosts by arbo-bunyaviruses from cellular and molecular perspectives, with particular attention to the human host. We address current knowledge and advances regarding the identity of the first-target cells and the subsequent processes of entry and penetration into the cytosol. Aspects of the vector-to-host switch that influence the early steps of cell infection in mammalian skin, where incoming particles are introduced by infected arthropods, are also highlighted and discussed.

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Segmented negative strand RNA virus nucleoprotein structure

Cited by 39 publications

References 47 publications

The RNA synthesis machinery of negative-stranded RNA viruses

The RNA synthesis machinery of negative-stranded RNA viruses

The catcher in the RIG-I

Bunyaviruses: From Transmission by Arthropods to Virus Entry into the Mammalian Host First-Target Cells

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