Segmented fluorescence correlation spectroscopy (FCS) on a commercial laser scanning microscope
Elisa Longo,
Silvia Scalisi,
Luca Lanzanò
Abstract:Performing accurate Fluorescence Correlation Spectroscopy (FCS) measurements in cells can be challenging due to cellular motion or other intracellular processes. In this respect, it has recently been shown that analysis of FCS data in short temporal segments (segmented FCS) can be very useful to increase the accuracy of FCS measurements inside cells. Here, we demonstrate that segmented FCS can be performed on a commercial laser scanning microscope (LSM), even in the absence of the dedicated FCS module. We show… Show more
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