2011
DOI: 10.1111/j.1365-2818.2011.03556.x
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Segmentation of virus particle candidates in transmission electron microscopy images

Abstract: Summary In this paper, we present an automatic segmentation method that detects virus particles of various shapes in transmission electron microscopy images. The method is based on a statistical analysis of local neighbourhoods of all the pixels in the image followed by an object width discrimination and finally, for elongated objects, a border refinement step. It requires only one input parameter, the approximate width of the virus particles searched for. The proposed method is evaluated on a large number of … Show more

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Cited by 46 publications
(24 citation statements)
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References 23 publications
(29 reference statements)
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“…In this section, we evaluate the performance of the IDDL scheme on eight computer vision datasets, which are known to benefit from SPD-based descriptors. To this end, we use the following datasets, namely (i) the JHMDB action recognition [24], (ii) the HMDB action recognition [26] (iii) the KTH-TIPS2 dataset [32], (iv) Brodatz textures [35], (v) the Virus dataset [28], (vi) the SHREC 3D shape dataset [30], (vii) the Myometrium cancer dataset [41], and (viii) the Breast cancer dataset [41]. Below, we provide details about all the studied datasets and the way SPD descriptors are obtained on them.…”
Section: Methodsmentioning
confidence: 99%
“…In this section, we evaluate the performance of the IDDL scheme on eight computer vision datasets, which are known to benefit from SPD-based descriptors. To this end, we use the following datasets, namely (i) the JHMDB action recognition [24], (ii) the HMDB action recognition [26] (iii) the KTH-TIPS2 dataset [32], (iv) Brodatz textures [35], (v) the Virus dataset [28], (vi) the SHREC 3D shape dataset [30], (vii) the Myometrium cancer dataset [41], and (viii) the Breast cancer dataset [41]. Below, we provide details about all the studied datasets and the way SPD descriptors are obtained on them.…”
Section: Methodsmentioning
confidence: 99%
“…The virus types range from 25 to 270 nm in diameter and their shapes vary from icosahedral to highly pleomorphic (for example like boiled spaghetti). The image patches are disk shaped cutouts centred on automatically segmented virus particles using the segmentation method presented in [14]. The viruses have been imaged at different magnifications in the TEM with a pixel size ranging from 0.5 to 5 nm.…”
Section: Methodsmentioning
confidence: 99%
“…PCA was used to ensure both HASC features and φ m (x i ) dimensionalities are compatible. We followed the original evaluation protocol with the provided 10 splits described in [17]. Specifically, for each repetition, one split was used as the test and the rest as training.…”
Section: Accepted Manuscriptmentioning
confidence: 99%
“…Analysis on EFS-DS and EFS-LR -The proposed EFS-DF Table 1. Average recognition accuracies and their standard deviations for the Object recognition (ETH80) [19], Action recognition (HDM05) [21], virus [17] and texture (Kylberg) datasets [16]. CDL, S H -SVM and HASC-SVM extract SPD manifold features, therefore cannot be used for Object Recognition dataset which uses Grassmann manifold.…”
Section: Accepted Manuscriptmentioning
confidence: 99%