Segmental chromosomal duplications harbouring group IV <i>CONSTANS</i>-like genes in cereals

Cockram, James; Howells, Rhian; O’Sullivan, Donal M.

doi:10.1139/g09-101

Cited by 16 publications

(24 citation statements)

References 30 publications

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“…Previous studies show B1 to be located on chromosome 5A, distal to the chromosomal 4AL/5AL breakpoint (Kato et al 1998). BLASTn analysis of the rice genome identifies the putative ortholog of the most significant SNP identified by mapping (BobWhite_c8266_227) to be LOC_Os03g01170.1 (5.1 e −111), located within a region previously shown to be collinear with the distal end of cereal group 4 chromosomes (Cockram et al 2010a), and thus involved in the 4AL/5AL translocation. Collectively, these results indicate that mapping in the MAGIC population has detected the B1 awning locus.…”

Section: Resultsmentioning

confidence: 99%

An Eight-Parent Multiparent Advanced Generation Inter-Cross Population for Winter-Sown Wheat: Creation, Properties, and Validation

Mackay

Bansept-Basler

Barber

et al. 2014

G3 Genes|Genomes|Genetics

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204

259

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MAGIC populations represent one of a new generation of crop genetic mapping resources combining high genetic recombination and diversity. We describe the creation and validation of an eight-parent MAGIC population consisting of 1091 F7 lines of winter-sown wheat (Triticum aestivum L.). Analyses based on genotypes from a 90,000-single nucleotide polymorphism (SNP) array find the population to be well-suited as a platform for fine-mapping quantitative trait loci (QTL) and gene isolation. Patterns of linkage disequilibrium (LD) show the population to be highly recombined; genetic marker diversity among the founders was 74% of that captured in a larger set of 64 wheat varieties, and 54% of SNPs segregating among the 64 lines also segregated among the eight founder lines. In contrast, a commonly used reference bi-parental population had only 54% of the diversity of the 64 varieties with 27% of SNPs segregating. We demonstrate the potential of this MAGIC resource by identifying a highly diagnostic marker for the morphological character "awn presence/absence" and independently validate it in an association-mapping panel. These analyses show this large, diverse, and highly recombined MAGIC population to be a powerful resource for the genetic dissection of target traits in wheat, and it is well-placed to efficiently exploit ongoing advances in phenomics and genomics. Genetic marker and trait data, together with instructions for access to seed, are available at http://www.niab.com/MAGIC/.

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Section: Resultsmentioning

confidence: 99%

An Eight-Parent Multiparent Advanced Generation Inter-Cross Population for Winter-Sown Wheat: Creation, Properties, and Validation

Mackay

Bansept-Basler

Barber

et al. 2014

G3 Genes|Genomes|Genetics

Self Cite

204

259

View full text Add to dashboard Cite

show abstract

“…The partial sequences of HvCO9 have previously been reported under the accession numbers, AY082965 (Griffiths et al , 2003) and FJ767842 and FJ 767852 (Cockram et al , 2010). Based on these data, the genomic sequences that covered the entire coding region of HvCO9 from two barley cultivars, Steptoe and Morex, were determined.…”

Section: Resultsmentioning

confidence: 99%

“…Partial sequences of HvCO9 had been reported previously under the accession numbers AY082965 (Griffiths et al , 2003) and FJ767842 and FJ 767852 (Cockram et al , 2010). To isolate the entire coding region of HvCO9 , total RNA was extracted from the leaves of cv.…”

Section: Methodsmentioning

confidence: 99%

The differential expression of HvCO9, a member of the CONSTANS-like gene family, contributes to the control of flowering under short-day conditions in barley

Kikuchi

Kawahigashi

Oshima

et al. 2011

Journal of Experimental Botany

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HvCO9 was characterized to elucidate the barley flowering control mechanisms and to investigate the functional diversification of the barley CONSTANS-like (CO-like) genes in flowering. HvCO9 was located on the same chromosome, 1HL, as Ppd-H2 (HvFT3), which is a positive regulator of short-day (SD) flowering. A phylogenetic analysis showed that HvCO9 was located on the same branch of the CO-like gene tree as rice Ghd7 and the barley and wheat VRN2 genes, which are all negative regulators of flowering. High level HvCO9 expressions were observed under SD conditions, whereas its expression levels were quite low under long-day (LD) conditions. HvCO9 expression correlated with HvFT1 and HvFT2 expression under SD conditions, although no clear effect of HvCO9 on HvFT3 expression, or vice versa, under SD conditions was observed. The over-expression of HvCO9 in rice plants produced a remarkable delay in flowering. In transgenic rice, the expression levels of the flowering-related Ehd1 gene, which is a target gene of Ghd7, and its downstream genes were suppressed, causing a delay in flowering. These results suggest that HvCO9 may act as a negative regulator of flowering under non-inductive SD conditions in barley; this activity is similar to that of rice Ghd7 under non-inductive LD conditions, but the functional targets of these genes may be different. Our results indicate that barley has developed its own pathways to control flowering by using homologous genes with modifications for the timing of expression. Further, it is hypothesized that each pathway may target different genes after gene duplication or species diversification.

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“…Additionally, VRN2 and Ghd7 genes are not present within colinear regions in Brachypodium (Cockram et al 2010;Higgins et al 2010). The presumed absence of VRN2 led to the hypothesis that Bd2I, a rapid-flowering line that does not require vemalization, may be rapid flowering because it lacks VRN2 and that delayed-fiowering Brachypodium accessions that show a vemalization requirement may contain a VRN2 ortholog (Cockram et al 2010;Higgins et al 2010). However, recent analyses of genome sequences of vemalization-requiring lines did not identify a sequence more related to VRN2 than Bradi3gl0010.…”

Section: Does Brachypodiumi Have An Ortholog Of Vrn2 From Wheat and Bmentioning

confidence: 98%

“…The next closest homolog in Brachypodium, Bradi3g33340, clearly resides in a well-supported C09 clade containing species spanning grass diversification (Cockram et al 2012). Additionally, VRN2 and Ghd7 genes are not present within colinear regions in Brachypodium (Cockram et al 2010;Higgins et al 2010). The presumed absence of VRN2 led to the hypothesis that Bd2I, a rapid-flowering line that does not require vemalization, may be rapid flowering because it lacks VRN2 and that delayed-fiowering Brachypodium accessions that show a vemalization requirement may contain a VRN2 ortholog (Cockram et al 2010;Higgins et al 2010).…”

Section: Does Brachypodiumi Have An Ortholog Of Vrn2 From Wheat and Bmentioning

confidence: 99%

The Molecular Basis of Vernalization in Different Plant Groups

Ream

Woods

Amasino

2012

Cold Spring Harbor Symposia on Quantitative Biology

102

116

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Timing of flowering is key to the reproductive success of many plants. In temperate climates, flowering is often coordinated with seasonal environmental cues such as temperature and photoperiod. Vernalization, the process by which a prolonged exposure to the cold of winter results in competence to flower during the following spring, is an example of the influence of temperature on the timing of flowering. In different groups of plants, there are distinct genes involved in vernalization, indicating that vernalization systems evolved independently in different plant groups. The convergent evolution of vernalization systems is not surprising given that angiosperm families had begun to diverge in warmer paleoclimates in which a vernalization response was not advantageous. Here, we review what is known of the vernalization response in three different plant groups: crucifers (Arabidopsis), Amaranthaceae (sugar beet), and Pooideae (wheat, barley, and Brachypodium distachyon). We also discuss the advantages of using Brachypodium as a model system to study flowering and vernalization in the Pooids. Finally, we discuss the evolution and function of the Ghd7/VRN2 gene family in grasses.

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Segmental chromosomal duplications harbouring group IV CONSTANS-like genes in cereals

Cited by 16 publications

References 30 publications

An Eight-Parent Multiparent Advanced Generation Inter-Cross Population for Winter-Sown Wheat: Creation, Properties, and Validation

An Eight-Parent Multiparent Advanced Generation Inter-Cross Population for Winter-Sown Wheat: Creation, Properties, and Validation

The differential expression of HvCO9, a member of the CONSTANS-like gene family, contributes to the control of flowering under short-day conditions in barley

The Molecular Basis of Vernalization in Different Plant Groups

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