Seesawed fluorescence nano-aptasensor based on highly vertical ZnO nanorods and three-dimensional quantitative fluorescence imaging for enhanced detection accuracy of ATP

Shrivastava, Sajal; Triet, Nguyen Minh; Son, Young-Min; Lee, Won‐Il; Lee, Nae‐Eung

doi:10.1016/j.bios.2016.09.089

Cited by 22 publications

(11 citation statements)

References 33 publications

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“…Metamaterials or composites combine the advantages of multiple elements in the nanoscale and have unique physico-chemical properties [ 1 , 2 , 3 ]. There is currently a great deal of interest in nanobio sensors where many of these applications involve zinc oxide (ZnO) nanoparticles (NPs) or doped derivatives [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. A variety of different target molecules, including but not limited to riboflavin, mucin-1, bisphenol A, ATP, acetamiprid, micro-RNA, thrombin, and different types of cancer cells (Hela, SK-BR-3, K562), have been detected [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…There is currently a great deal of interest in nanobio sensors where many of these applications involve zinc oxide (ZnO) nanoparticles (NPs) or doped derivatives [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. A variety of different target molecules, including but not limited to riboflavin, mucin-1, bisphenol A, ATP, acetamiprid, micro-RNA, thrombin, and different types of cancer cells (Hela, SK-BR-3, K562), have been detected [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. ZnO has been fabricated into a variety of different structures with various other components including carbon quantum dots [ 4 ], platinum [ 5 ], AlGaN [ 6 ], Au (gold) [ 7 , 8 , 11 ], Co (cobalt) [ 9 ], and graphene [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…A variety of different target molecules, including but not limited to riboflavin, mucin-1, bisphenol A, ATP, acetamiprid, micro-RNA, thrombin, and different types of cancer cells (Hela, SK-BR-3, K562), have been detected [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. ZnO has been fabricated into a variety of different structures with various other components including carbon quantum dots [ 4 ], platinum [ 5 ], AlGaN [ 6 ], Au (gold) [ 7 , 8 , 11 ], Co (cobalt) [ 9 ], and graphene [ 12 ]. In many cases, detection by these nanocomposite sensors is based on electrochemistry or photoelectrochemistry [ 5 , 7 , 9 , 11 , 12 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…In many cases, detection by these nanocomposite sensors is based on electrochemistry or photoelectrochemistry [ 5 , 7 , 9 , 11 , 12 , 14 ]. However, in some cases, detection has been based on electrochemiluminescence [ 4 ], field effect transistors (FET) [ 13 ], or 3-D quantitative fluorescence imaging [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Two-Dimensional Fluorescence Difference Spectroscopy of ZnO and Mg Composites in the Detection of Physiological Protein and RNA Interactions

Hoffman

Wang

et al. 2017

Materials

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Two-dimensional fluorescence difference spectroscopy (2-D FDS) was used to determine the unique spectral signatures of zinc oxide (ZnO), magnesium oxide (MgO), and 5% magnesium zinc oxide nanocomposite (5% Mg/ZnO) and was then used to demonstrate the change in spectral signature that occurs when physiologically important proteins, such as angiotensin-converting enzyme (ACE) and ribonuclease A (RNase A), interact with ZnO nanoparticles (NPs). When RNase A is bound to 5% Mg/ZnO, the intensity is quenched, while the intensity is magnified and a significant shift is seen when torula yeast RNA (TYRNA) is bound to RNase A and 5% Mg/ZnO. The intensity of 5% Mg/ZnO is quenched also when thrombin and thrombin aptamer are bound to the nanocomposite. These data indicate that RNA–protein interaction can occur unimpeded on the surface of NPs, which was confirmed by gel electrophoresis, and importantly that the change in fluorescence excitation, emission, and intensity shown by 2-D FDS may indicate specificity of biomolecular interactions.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Two-Dimensional Fluorescence Difference Spectroscopy of ZnO and Mg Composites in the Detection of Physiological Protein and RNA Interactions

Hoffman

Wang

et al. 2017

Materials

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“…ZnO has been previously used in biosensing FETs, both in the form of thin films [120][121][122][123] and as nanowires [19,103,[124][125][126]. The adhesion of aptamers to ZnO typically requires a linker molecule.…”

Section: Zno Biosensorsmentioning

confidence: 99%

Laterally Grown ZnO Nanowires for Sensing Applications

Matthews¹

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<p>Zinc oxide nanowires are a semiconducting material that has many uses in electronic applications. In particular, ZnO nanowires have been used in field effect transistors and applied as sensors for the detection of gases, biomolecules, UV light and as pressure sensors. ZnO nanowires can be fabricated using many different methods, but most require the use of high tempertures and have extensive setup costs. Hydrothermal growth, however, provides a cheap and low temperture method for growing ZnO nanowires. Much work has been done on the synthesis and charcetristaion of ZnO nanowires grown using hydrothermal growth, in partiuclar for photovoltaic applications. Little work has been done on the performace of hydrothermally grown ZnO nanowires in field effect transtors. This thesis looks at applying hydrothermally grown ZnO nanowires as field effect transistors (FET). The FETs are characterised and developed with the intention of using them in senseing applications. The nanowire FET structure is optimised for sensing by developing a method that constrains the nanowires to exclusively lateral growth. A Ti capping layer is fabricated on top of a ZnO seed layer. The ZnO seed layer is then etched with dilute acid so that the Ti layer overhangs the ZnO. This acts as a physical barrier to vertical wire growth from the ZnO seed layer. The maximum deviation of the nanowires from the horizontal can be controlled by etching for different times. Two device types are fabricated using different size nanowires. One uses large nanowires, or nanorods (diameter 400 nm), while the second device type uses a hybrid structure of large nanorods with much thinner nanowires (diameter 20 nm) growing off them. Both device types are characterised as FETs in dry conditions and also when immersed in a number of different liquids. Two different gating setups are also used with the Si/SiO₂ substrate used as a backgate and a Ag/AgCl electrode inserted into liquid as a topgate. The large nanorods only show field dependence when wet due to the large capacitance of the elctric double layer and enhanced band bending. The wet nanorods can achieve on/off ratios of 10³. In contrast, the thinner nanowires show field dependence both when dry and when wet. On/off ratios of more than 10⁴ are achieved. In general the nanowires have superior on off ratios and smaller off current due to their larger surface to volume ratio. Attempts are made to functionalise the nanowires with aptamers so that they can be used as a biosensor. The functionalisation procedure is documented, however the overall procedure proves to be unsuccessful due to the instability and dissolution of the nanowires in tris buffer. The rate of decay in buffer solution is investigated. Both device types are also tested as gas sensors for humidity and ethanol detection. The nanorods show no apparent detection, while the nanowires show some response to ethanol. Further development of the experimental setup is necessary to better characterise the devices. Finally future work on these nanowires is discussed and possible improvements proposed for future development as biosensors and gas sensors.</p>

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A Multicolor Chameleon DNA‐templated Silver Nanocluster and Its Application for Ratiometric Fluorescence Target Detection with Exponential Signal Response

Zhou

Zhu

Fan

et al. 2017

Adv Funct Materials

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For the first time, a novel type of chameleon DNA-templated silver nanocluster (AgNC) is found whose fluorescence color can be switched among yellow, orange, and red by the regulation of complementary DNA, nonfluorescent assistant AgNC as well as Mg 2+ . AgNC templated by A 20 -C55 (A 20 -C55-NC) possesses strong yellow fluorescence (Y signal) in phosphate buffer solution. When approaching to the nonfluorescent assistant AgNC through template hybridization, Y signal decreases while a new red emission (R signal) rises, leading to a dramatic color change of AgNC solution from yellow to red. On the other hand, hybridization of A 20 -C55-NC with complementary DNA (T 20 ) largely enhances the Y signal while A 20 -C55-NC shows R and Y signal with equal intensity simultaneously in the presence of Mg 2+ . Therefore, the chameleon AgNC achieves controllable multicolor fluorescence variation. Based on above mechanism, a series of ratiometric analysis platforms are constructed for DNA target detection. Surprisingly, the ratiometric probes demonstrate an exponential growth of signal response with nanomolar sensitivity whether in double-stranded or hairpin-shaped structure. Accordingly, this universal ratiometric analysis platform possesses low background, large signal variation in a narrow concentration range, which presents obvious advantages over most of previous DNA detection strategies that are based on DNA-templated AgNC.

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Seesawed fluorescence nano-aptasensor based on highly vertical ZnO nanorods and three-dimensional quantitative fluorescence imaging for enhanced detection accuracy of ATP

Cited by 22 publications

References 33 publications

Two-Dimensional Fluorescence Difference Spectroscopy of ZnO and Mg Composites in the Detection of Physiological Protein and RNA Interactions

Two-Dimensional Fluorescence Difference Spectroscopy of ZnO and Mg Composites in the Detection of Physiological Protein and RNA Interactions

Laterally Grown ZnO Nanowires for Sensing Applications

A Multicolor Chameleon DNA‐templated Silver Nanocluster and Its Application for Ratiometric Fluorescence Target Detection with Exponential Signal Response

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