Seeking universal detectors for analytical characterizations

Zhang, Kelly; Kurita, Kenji L.; Venkatramani, Cadapakam J.; Russell, David J.

doi:10.1016/j.jpba.2018.09.029

Cited by 43 publications

(21 citation statements)

References 122 publications

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“…Evaporating light-scattering detection (ELSD), first described by Charlesworth [37], is a quasiuniversal detector than can detect any analyte less volatile than the mobile phase [38,39].…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a novel UPLC-ELSD method for the assessment of lipid composition of nanomedicine formulation

Varache

Ciancone

Couffin

2019

International Journal of Pharmaceutics

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Development and validation of a novel UPLC-ELSD method for the assessment of lipid composition of nanomedicine formulation.

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“…Evaporating light-scattering detection (ELSD), first described by Charlesworth [37], is a quasiuniversal detector than can detect any analyte less volatile than the mobile phase [38,39].…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a novel UPLC-ELSD method for the assessment of lipid composition of nanomedicine formulation

Varache

Ciancone

Couffin

2019

International Journal of Pharmaceutics

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“…Theoretically, the correlation of peak area with fraction amount is positive but may not be linear, and is likely to be a single logarithm or double logarithm on ELSD (Dvořáčková, Šnóblová, & Hrdlička, 2014; Elder, Borman, & Okafo, 2015; Zhang, Kurita, Venkatramani, & Russell, 2019). When sample amount is varied in a limited range, the correlation of peak area with fraction amount can be linearly regressed with an acceptable R 2 .…”

Section: Resultsmentioning

confidence: 99%

The determination of lycopene Z‐isomer absorption coefficient on C30‐HPLC

Huang

Bo-di

2020

Food Science & Nutrition

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Lycopene is a naturally occurring carotene composed solely of carbon and hydrogen atoms with the semisystem nomenclature of, ψ-ψcarotene, molecular formula of C 40 H 56 , and relative molecular weight of 536.87 Da. Lycopene can be biologically synthesized as a natural product in higher plants and microorganisms, and chemically synthesized as a synthetic product. Both of them have been demonstrated to exhibit various health functions in vivo (Costa

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“…The sample size was big enough as suggested (> 100) to get a stable result for LTG but not for MHD (Bland, 2004). HPLC-UV was higher than UHPLC-MS/MS for LTG analysis with a mean value of 0.6 mg/L, which could be explained by the co-elution of endogenous impurities attributed to the well-known less specificity of UV detector (Zhang et al, 2019). Quantitative microsphere system LTG immunoassay was applied in the clinical practice and compared with HPLC-UV method in the previous studies (Westley & Morris, 2008;Baldelli et al, 2015a;Morgan et al, 2011).…”

Section: Methods Application and Comparisonmentioning

confidence: 95%

“…Thus, isocratic elution by methanol-water was used for separation. The use of potassium dihydrogen phosphate in water (20-100 mM) could increase the intensity and shorten the retention time of three analytes (Additional file 1: Appendix 6) (Greiner-Sosanko et al, 2007;Contin et al, 2010;Rivas et al, 2010;Kimiskidis et al, 2007;Zhang et al, 2019). In summary, isocratic elution with methanol to water-50 mM potassium dihydrogen phosphate at 39:61 was used for separation, and 210 nm was used for detection to get acceptable peak shape, sensitivity, and run time.…”

Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 99%

Development and validation of an improved HPLC-UV method for simultaneous determination of lamotrigine and oxcarbazepine and its active metabolite 10,11-dihydro-10-hydroxycarbazepine in human blood plasma and comparison with an UHPLC-MS/MS method

et al. 2019

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Lamotrigine (LTG) and oxcarbazepine (OXC) are first-line drugs for epilepsy treatment. Their large pharmacokinetics variabilities and relations between efficacy and toxicity and blood plasma concentration require routine monitoring for dose adjustment. In this study, we developed and validated a simple, accurate, and reliable method for simultaneous determination of LTG, OXC and 10,11-dihydro-10-hydroxycarbazepine (MHD) in human blood plasma by high-performance liquid chromatography-ultraviolet detection (HPLC-UV) with a simple one-step protein precipitation using methanol (1% acetic acid) and 15 min elution time under isocratic elution at 1 mL/min. Calibration range was 2.4 to 120 mg/L for LTG, OXC, and MHD. The intra-day and inter-day bias were − 8.84 to 4.18%, and the imprecision was less than 8.08% for all analytes. The internal standard (fluconazole) normalized recovery was 96.30 to 107.69% for LTG, 98.51 to 111.04% for MHD, and 95.04 to 109.86% for OXC. A total of 186 LTG samples and 25 MHD samples were used to evaluate the agreement between HPLC-UV and ultra-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) by Passing-Bablok regression and Bland-Altman plot. The mean bias and the 95% limits of agreement (95% LOA) of the two measurements were 0.575 mg/L and − 1.238 to 2.387 mg/L for LTG (n = 186) and − 1.222 mg/L and − 8.271 to 5.827 mg/L for MHD (n = 25), which indicated the UV method was comparable with the MS method for LTG and MHD analysis.

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Seeking universal detectors for analytical characterizations

Cited by 43 publications

References 122 publications

Development and validation of a novel UPLC-ELSD method for the assessment of lipid composition of nanomedicine formulation

Development and validation of a novel UPLC-ELSD method for the assessment of lipid composition of nanomedicine formulation

The determination of lycopene Z‐isomer absorption coefficient on C30‐HPLC

Development and validation of an improved HPLC-UV method for simultaneous determination of lamotrigine and oxcarbazepine and its active metabolite 10,11-dihydro-10-hydroxycarbazepine in human blood plasma and comparison with an UHPLC-MS/MS method

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