Seeing the Full Picture: Advances in 3D Cell Culture Microscopy

Martin, Joseph

doi:10.2144/btn-2020-0094

Cited by 6 publications

(4 citation statements)

References 20 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Morohaku et al 27 established a protocol to obtain in vitro mouse oocytes from PGCs. However, the approach precludes a cytological 28 and metabolic assessment of the differentiation process and analysis of the effects of potentially toxic compounds as we have performed. For these reasons, we have modified some aspects of the protocol, mainly the supports of the 3D cultures.…”

Section: Resultsmentioning

confidence: 99%

The antiandrogenic vinclozolin induces differentiation delay of germ cells and changes in energy metabolism in 3D cultures of fetal ovaries

González-Sanz

Barreñada

Rial

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Vinclozolin is a pesticide with antiandrogenic activity as an endocrine disruptor compound. Its effects upon the progression of primordial follicles were assessed in cultures of mouse fetal ovaries from the onset of meiotic differentiation of germ cells (13.5 days post coitum) and from both in vivo exposed mice and in vitro exposed ovaries. Exposure of ovaries to vinclozolin—at in vitro dosages ranging from 10 to 200 μM and in 3D ex vivo culture following in vivo exposure to 50 mg/kg bw/day—showed delays in meiocyte differentiation and in follicle growth, even at the lowest in vitro dose exposure. Immunofluorescent analysis showed the presence of the proteins MSY2 and NOBOX in the primary follicles but no difference in the level of protein signals or in the number of follicles in relation to treatment. However, assessing the cytological differentiation of germ cells by detecting the synaptonemal complex protein SYCP3, the exposure to vinclozolin delayed meiotic differentiation from both in vitro- and in vivo-exposed ovaries. These effects were concomitant with changes in the energy metabolism, detected as a relative increase of glycolytic metabolism in live-cell metabolic assays in exposed ovaries.

show abstract

Section: Resultsmentioning

confidence: 99%

The antiandrogenic vinclozolin induces differentiation delay of germ cells and changes in energy metabolism in 3D cultures of fetal ovaries

González-Sanz

Barreñada

Rial

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The most significant drawback is that cell culture models may fail to replicate in vivo biology since they lack the complex network of interactions or intercellular signaling, which occurs in tissues, organs or whole organisms. Recent advances do include co-cultures of multiple cell types and researchers are increasingly using 3D cell models to recreate organized, miniaturized versions of organs to study diseases ( Martin 2020 ). All three types of imaging ways have their advantages and disadvantages as well as technical difficulties and necessary equipment (reviewed in ( Hickey et al, 2022 )).…”

Section: How To Choose the Right Imaging Technique For Your Intercell...mentioning

confidence: 99%

“…The axial resolution of most light-based techniques is not enough when imaging deeper in the cells. Next to growing cells in 3D [like organoids ( Martin 2020 ; Hickey et al, 2022 )], imaging epithelial or endothelial tissue sections is another good approach. Tissue sections have the major advantage of having preserved complex interactions among cells and their microenvironment and therefore cells kept their cell polarity in the hierarchical architecture of the tissue.…”

Section: How To Choose the Right Imaging Technique For Your Intercell...mentioning

confidence: 99%

Microscopic Visualization of Cell-Cell Adhesion Complexes at Micro and Nanoscale

Vanslembrouck

Chen

Larabell

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Considerable progress has been made in our knowledge of the morphological and functional varieties of anchoring junctions. Cell-cell adhesion contacts consist of discrete junctional structures responsible for the mechanical coupling of cytoskeletons and allow the transmission of mechanical signals across the cell collective. The three main adhesion complexes are adherens junctions, tight junctions, and desmosomes. Microscopy has played a fundamental role in understanding these adhesion complexes on different levels in both physiological and pathological conditions. In this review, we discuss the main light and electron microscopy techniques used to unravel the structure and composition of the three cell-cell contacts in epithelial and endothelial cells. It functions as a guide to pick the appropriate imaging technique(s) for the adhesion complexes of interest. We also point out the latest techniques that have emerged. At the end, we discuss the problems investigators encounter during their cell-cell adhesion research using microscopic techniques.

show abstract

“…With the increasing complexity of 3D cell models, there is a need for more sophisticated tools to analyze them. However, the majority of analysis techniques currently in use were originally developed for 2D cell cultures, making the transition to 3D less than ideal [ 194 ].…”

Section: Discussionmentioning

confidence: 99%

3D Cell Models in Radiobiology: Improving the Predictive Value of In Vitro Research

Antonelli

2023

IJMS

View full text Add to dashboard Cite

Cancer is intrinsically complex, comprising both heterogeneous cellular composition and extracellular matrix. In vitro cancer research models have been widely used in the past to model and study cancer. Although two-dimensional (2D) cell culture models have traditionally been used for cancer research, they have many limitations, such as the disturbance of interactions between cellular and extracellular environments and changes in cell morphology, polarity, division mechanism, differentiation and cell motion. Moreover, 2D cell models are usually monotypic. This implies that 2D tumor models are ineffective at accurately recapitulating complex aspects of tumor cell growth, as well as their radiation responses. Over the past decade there has been significant uptake of three-dimensional (3D) in vitro models by cancer researchers, highlighting a complementary model for studies of radiation effects on tumors, especially in conjunction with chemotherapy. The introduction of 3D cell culture approaches aims to model in vivo tissue interactions with radiation by positioning itself halfway between 2D cell and animal models, and thus opening up new possibilities in the study of radiation response mechanisms of healthy and tumor tissues.

show abstract

Seeing the Full Picture: Advances in 3D Cell Culture Microscopy

Abstract: As 3D cell models become increasingly intricate, they require more complex tools to analyze them. How are microscopy techniques keeping up with this rapidly evolving field?

Cited by 6 publications

References 20 publications

The antiandrogenic vinclozolin induces differentiation delay of germ cells and changes in energy metabolism in 3D cultures of fetal ovaries

The antiandrogenic vinclozolin induces differentiation delay of germ cells and changes in energy metabolism in 3D cultures of fetal ovaries

Microscopic Visualization of Cell-Cell Adhesion Complexes at Micro and Nanoscale

3D Cell Models in Radiobiology: Improving the Predictive Value of In Vitro Research

Contact Info

Product

Resources

About