The dimensionality limitation of human spatial representations has been a long-lasting, unsolved issue in psychology, mathematics, and philosophy. The present study examined the possibility of human four-dimensional spatial representations using a spatial judgment task on hyper-volume, a novel property unique to higher dimensional space. Observers studied visual simulations of random wireframe hypertetrahedrons (4-simplexes) rotating around the y-z plane and judged their hyper-volume by adjusting the size of a 4-D block. Multiple regression analyses showed a significant correlation between the responses and the actual hyper-volume but not the definitionbased, lower-dimensional cues such as the mean 3-D volume, providing empirical evidence of human 4-D spatial representations that can support judgments of certain novel, high-dimensional properties.