Seeded growth of hydroxyapatite in simulated body fluid

Spanos, N.; Misirlis, Dimitrios; Kanellopoulou, Dimitra G.; Koutsoukos, Petros G.

doi:10.1007/s10853-006-2952-9

Cited by 41 publications

(32 citation statements)

References 32 publications

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“…Once the apatite nuclei are formed, they grow by uptake of calcium and phosphate ions from the solution. 58, 63 …”

Section: Discussionmentioning

confidence: 99%

Bio-Templated Growth of Bone Minerals from Modified Simulated Body Fluid on Nanofibrous Decellularized Natural Tissues

Yang¹,

Wang²,

Zhu³

et al. 2016

j biomed nanotechnol

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Small intestine submucosal (SIS) membrane used in this study is a decellularized, naturally occurring nanofibrous scaffold derived from a submucosal layer of porcine small intestine. It is predominantly composed of type I collagen fibers. Here we studied the bio-templated growth of hydroxylapatite (HAP) bone minerals on the SIS membrane from a modified simulated body fluid (1.5 SBF) at the body temperature, namely, under a near-physiological condition, in order to evaluate its bone bioactivity, the capability of the membrane in bonding with bone tissue once implanted in vivo. Minute HAP crystals were successfully nucleated on the SIS membranes from 1.5 SBF at the body temperature. The crystals were preferentially nucleated along the collagen fibers constituting the SIS membranes. HAP was the major crystalline mineral phase formed during the whole period of time and a minor crystalline phase of tricalcium phosphate (TCP) appeared after the membranes were incubated for 96 h. We also found that the mineralization for 8 h most significantly promoted the osteogenic differentiation of rat mesenchymal stem cells (MSCs) by evaluating the formation of osteogenic markers in MSCs including alkaline phosphatase (early stage marker) as well as osteocalcin and osteopontin (late stage markers). Hence, SIS membranes show excellent bone bioactivity and once mineralized, can significantly promote the osteogenic differentiation of MSCs.

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“…Once the apatite nuclei are formed, they grow by uptake of calcium and phosphate ions from the solution. 58, 63 …”

Section: Discussionmentioning

confidence: 99%

Bio-Templated Growth of Bone Minerals from Modified Simulated Body Fluid on Nanofibrous Decellularized Natural Tissues

Yang¹,

Wang²,

Zhu³

et al. 2016

j biomed nanotechnol

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“…For example, DCPD is found as an intermediate phase during topical fluoride treatment, and may ultimately convert to FAp [Chow and Brown, 1973, 1975]. A coating of DCPD has been reported to form on HAp for solution pH 4-6 [Francis, 1965], and it may also occur transiently [Neuman and Bareham, 1975;Johnsson and Nancollas, 1992;Spanos et al, 2006]. Indeed, DCPD has been postulated as being intimately involved in the development of the surface layer of a caries lesion [Margolis and Moreno, 1985].…”

mentioning

confidence: 99%

Solubility of Dicalcium Phosphate Dihydrate by Solid Titration

Pan

Darvell

2009

Caries Res

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Solid-titration results for hydroxyapatite (HAp), octacalcium phosphate, β-tricalcium phosphate and tetracalcium phosphate have shown that the only stable phase in 100 mmol·l^-1 KCl at 37°C is HAp. In particular, dicalcium phosphate dihydrate (DCPD) did not form at pH <4.2 (where it is otherwise believed to be stable) except as a metastable phase under conditions of slight supersaturation. The behaviour of DCPD itself under the same conditions requires checking. Solid titration was used to determine the apparent solubility of DCPD in a 100-mmol·l^–1 KCl solution at 37.0 ± 0.1°C over the pH range 3.2–11.6. The constitution of the precipitate was determined by X-ray diffraction, particle morphology was observed by scanning and transmission electron microscopy, and the precipitate Ca/P ratio was calculated by energy-dispersive X-ray analysis. The titration curve for DCPD was substantially lower than the position reported elsewhere. DCPD was the only identified phase at equilibrium at pH 3.60 and 4.50, but HAp was formed after seeding with 1 mg HAp at DCPD equilibrium at pH 4.47, 3.60 and 3.30. It is concluded that the titration curve observed for DCPD corresponds to the solubility isotherm for the phase, but that this represents a metastable equilibrium. HAp is more stable than DCPD, particularly below pH 4.2. The implications for calcium phosphate studies are profound as the reverse is generally believed to be true. Thus, solubility results and the nature of the carious lesion need reconsideration.

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“…Besides, as stated in the literature [5,52,56,67] , the lack of OH -could also be partly attributed to the demands of charge balance created by the replacement of PO 4 3- There is growing evidence for the lack of OH -in bone apatite, based on results from…”

Section: Bioactivitymentioning

confidence: 98%

“…6 ), which may transform in a second step into calcium-deficient apatite or hydroxyapatite [57,58] . The calcium phosphate system is complicated as it involves a number of salts of variable stability [56] , but HAp is the only thermodynamically stable calcium phosphate that exists in aqueous solutions at pH greater than 4.2 [59] .…”

Section: Biomimetic In Vitro Test For Predicting the In Vivo Bioactivitymentioning

confidence: 99%

“…Apatites deposited in a solution often replace the Ca 2+ ion site with small amounts of Na + , Mg 2+ or K + ions, which lower the Ca/P molar ratios [56] . The presence of these ions in the crystalline network of the apatite was evaluated by the (Ca+Mg+Na+K)/P ratio, though a portion could be merely adsorbed onto the surface of the apatite [54] .…”

Section: Bioactivitymentioning

confidence: 99%

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P(EMA-co-HEA)/SiO2 hybrid nanocomposites for guided dentin tissue regeneration: structure, characterization and bioactivity.

Vallés-Lluch¹

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Seeded growth of hydroxyapatite in simulated body fluid

Cited by 41 publications

References 32 publications

Bio-Templated Growth of Bone Minerals from Modified Simulated Body Fluid on Nanofibrous Decellularized Natural Tissues

Bio-Templated Growth of Bone Minerals from Modified Simulated Body Fluid on Nanofibrous Decellularized Natural Tissues

Solubility of Dicalcium Phosphate Dihydrate by Solid Titration

P(EMA-co-HEA)/SiO2 hybrid nanocomposites for guided dentin tissue regeneration: structure, characterization and bioactivity.

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