SeedCalc, a new automated R software tool for germination and seedling length data processing

Silva, Laércio Junio da; Medeiros, André Dantas de; Oliveira, Ariadne Morbeck Santos

doi:10.1590/2317-1545v42n2217267

Cited by 46 publications

(51 citation statements)

References 8 publications

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“…The software provided measurements of shoot length (SL), primary root length (RL), and total length (TL) of seedlings. After that, the seedling length values obtained by SAPL® were processed using the SeedCalc package of the R software (Silva, Medeiros, & Oliveira, 2019a) to generate the uniformity (Castan et al, 2018), growth (Medeiros, Silva, Pereira, Oliveira, & Dias, 2020), and corrected vigor indices.…”

Section: Seedlingmentioning

confidence: 99%

Chickpea seed vigor evaluated by computerized seedling analysis

Araújo

Dias

Medeiros

et al. 2021

Semina: Ciênc. Agrár.

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Computerized seedling analysis is a promising tool for assessing seed vigor, providing reliable and less subjective results. The aim of this study was to define a method for evaluating the vigor of chickpea seeds through computerized image analysis, using the Seedling Analysis System (Sistema de Análise de Plântula - SAPL®) as a basis. Two chickpea cultivars were evaluated, consisting of five seed lots of cv. BRS Aleppo and four seed lots of cv. Cícero. Test were performed on the seeds of each cultivar for initial characterization of the physiological potential of the lots, and the results were compared with the determinations made via SAPL®. The results showed high correlations between the variables obtained from computerized analysis and from traditional vigor tests, especially for the close relationship between the cold test and low temperature germination test with the corrected vigor index (r > 0.9) evaluated on the fourth day after the beginning of the test. The total length of seedlings, length of the primary root, and growth and corrected vigor indices obtained through computerized analysis of seedlings on the 4th day after sowing allowed separation of the lots regarding vigor. Computerized analysis of seedlings using SAPL® is effective in determining the vigor of chickpea seed lots.

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Section: Seedlingmentioning

confidence: 99%

Chickpea seed vigor evaluated by computerized seedling analysis

Araújo

Dias

Medeiros

et al. 2021

Semina: Ciênc. Agrár.

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“…Com os dados obtidos foi determinado o tempo médio de germinação (TMG), o qual é expresso em dias e calculado através da equação proposta por Labouriau (1983), considerando o número de sementes germinadas no intervalo entre cada contagem (ni) e o tempo decorrido entre o início da germinação e a i-ésima contagem (ti), sendo: Silva et al (2019). A avaliação do comprimento e massa seca de plântulas foi realizada em diferentes períodos de tempo (96, 120, 144 e 168 horas após a montagem do teste).…”

Section: Classes De Tamanhounclassified

QUALIDADE FISIOLÓGICA DE SEMENTES DE Peltophorum dubium (Sprengel.) Taubert CLASSIFICADAS PELO TAMANHO

Padilha¹,

Donatto²,

Sobral³

2021

BIOFIX

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Uma estratégia adaptativa das espécies florestais contra fatores imprevisíveis do ambiente é a oscilação na produção anual e no tamanho das sementes. Neste sentido, diversos estudos têm buscado investigar a influência do tamanho das sementes na germinação e vigor. As pesquisas sobre a relação entre tamanho e qualidade fisiológica das sementes podem contribuir para a criação de estratégias para a seleção de sementes destinadas ao processo de produção de mudas. O trabalho teve como objetivo avaliar a qualidade fisiológica das sementes de Peltophorum dubium classificadas pelo tamanho. O lote de sementes foi separado e pesado em três classes de tamanho: pequenas (< 0,0501 g), médias (0,0501 - 0,0600 g) e grandes (> 0,0600 g). O delineamento experimental foi o inteiramente casualizado com oito repetições. Os parâmetros avaliados foram: plântulas normais e anormais, sementes duras e mortas, primeira contagem de germinação, índice de velocidade de germinação, tempo médio de germinação, uniformidade de germinação, comprimento de plântulas, massa seca de plântulas e índice de vigor. Houve diferença significativa entre as variáveis analisadas, sendo possível identificar diferenças na qualidade fisiológica entre as classes de tamanho. Com os resultados foi possível identificar que as sementes com menor tamanho apresentaram maior intensidade de dormência; as sementes de tamanho médio demonstraram maior qualidade fisiológica e, as sementes de tamanho grande apresentam maior potencial para formação de plântulas com maior massa seca.

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“…Daily counts of the number of seeds exhibiting primary root protrusion and germinated seeds (normal seedlings) were carried out until the eighth day after sowing. From these data, the speed of germination index (GSI), the speed of primary root protrusion (RPS) and the synchrony were calculated, according to the formulas described in Silva et al (2019). All the variables obtained based on the germination test were calculated using the Germcalc function, contained in the package SeedCalc of the R software.…”

Section: Physiological Analysismentioning

confidence: 99%

X-ray imaging and digital processing application in non-destructive assessing of melon seed quality

et al. 2020

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Non-destructive and high throughput methods have been developed for seed quality evaluation. The aim of this study was to relate parameters obtained from the free and automated analysis of digital radiographs of hybrid melons’ seeds to their seeds’ physiological potential. Seeds of three hybrid melon (Cucumis melo L.) cultivars from commercial lot samples were used. Radiographic images of the seeds were obtained, from which area, perimeter, circularity, relative density, integrated density and seed filling measurements were generated by means of a macro (PhenoXray) developed for ImageJ® software. After the X-ray test, seed samples were submitted to the germination test, from which variables related to the physiological quality of the seeds were obtained. Variability between lots was observed for both physical and physiological characteristics. Results showed that the use of the PhenoXray macro allows large-scale phenotyping of seed radiographs in a simple, fast, consistent and completely free way. The methodology is efficient in obtaining morphometric and tissue integrity data of melon seeds and the generated parameters are closely related to physiological attributes of seed quality.

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SeedCalc, a new automated R software tool for germination and seedling length data processing

Cited by 46 publications

References 8 publications

Chickpea seed vigor evaluated by computerized seedling analysis

Chickpea seed vigor evaluated by computerized seedling analysis

QUALIDADE FISIOLÓGICA DE SEMENTES DE Peltophorum dubium (Sprengel.) Taubert CLASSIFICADAS PELO TAMANHO

X-ray imaging and digital processing application in non-destructive assessing of melon seed quality

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