Seed Treatments for Eradicating<i>Clavibacter michiganensis</i>subsp.<i>michiganensis</i>from Naturally Infected Tomato Seeds

Fatmi, M.; Schaad, N. W.; Bolkan, H. A.

doi:10.1094/pd-75-0383

Cited by 49 publications

(26 citation statements)

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“…C. michiganensis subsp. michiganensis can also colonize the fruit and seed, and infected seed are a major source of disease spreading (Fatmi et al 1991;Tsiantos 1987). Although several wild tomato species have been shown to be resistant to C. michiganensis subsp.…”

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confidence: 99%

Clavibacter michiganensis subsp. michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato

Savidor¹,

Chalupowicz²,

Teper³

et al. 2014

MPMI

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The plant pathogen Clavibacter michiganensis subsp. michiganensis is a gram-positive bacterium responsible for wilt and canker disease of tomato. Although disease development is well characterized and diagnosed, molecular mechanisms of C. michiganensis subsp. michiganensis virulence are poorly understood. Here, we identified and characterized two C. michiganensis subsp. michiganensis transcriptional regulators, Vatr1 and Vatr2, that are involved in pathogenicity of C. michiganensis subsp. michiganensis. Vatr1 and Vatr2 belong to TetR and MocR families of transcriptional regulators, respectively. Mutations in their corresponding genes caused attenuated virulence, with the Δvatr2 mutant showing a more dramatic effect than Δvatr1. Although both mutants grew well in vitro and reached a high titer in planta, they caused reduced wilting and canker development in infected plants compared with the wild-type bacterium. They also led to a reduced expression of the ethylene-synthesizing tomato enzyme ACC-oxidase compared with wild-type C. michiganensis subsp. michiganensis and to reduced ethylene production in the plant. Transcriptomic analysis of wild-type C. michiganensis subsp. michiganensis and the two mutants under infection-mimicking conditions revealed that Vatr1 and Vatr2 regulate expression of virulence factors, membrane and secreted proteins, and signal-transducing proteins. A 70% overlap between the sets of genes positively regulated by Vatr1 and Vatr2 suggests that these transcriptional regulators are on the same molecular pathway responsible for C. michiganensis subsp. michiganensis virulence.

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Clavibacter michiganensis subsp. michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato

Savidor¹,

Chalupowicz²,

Teper³

et al. 2014

MPMI

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“…Ácido clorídrico foi empregado TABELA 1 -Porcentagem de controle de Leifsonia xyli subsp. xyli (Lxx) detectada por "dot blot" e brotação de toletes de cana-de-açúcar em função de diferentes tratamentos térmicos (Fatmi et al, 1991). Ácido peracético é um poderoso desinfetante/esterilizante hospitalar com amplo e rápido espectro de ação e baixo custo (Svidzinski et al, 2007) e que também foi recentemente introduzido na esterilização dos equipamentos da indústria de alimentos no Brasil pois tem a vantagem de ser atóxico e biodegradável (Kunigk et al, 2001), mas que, aparentemente, ainda não foi testado como alternativa para o controle de patógenos de sementes ou outros órgãos propagativos.…”

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Avaliação de diferentes tratamentos térmicos no controle do raquitismo-da-soqueira em cana-de-açúcar

Júnior¹,

Júnior²,

Marchetti³

et al. 2010

Trop. plant pathol.

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“…ssp. michiganensis from infested seeds (Blood, 1937; Thyr et al ., 1973; Shoemaker & Echandi, 1976; Dhanvantari, 1989; Fatmi et al ., 1991) and are standard practices in the seed industry. If C .…”

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confidence: 99%

Effects of nutrient solution pH on the survival and transmission of Clavibacter michiganensis ssp. michiganensis in hydroponically grown tomatoes

Huang

2001

Plant Pathology

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The effect of pH on the survival of Clavibacter michiganensis ssp. michiganensis and its transmission via roots of tomato in hydroponic culture was studied in laboratory and greenhouse experiments. In a laboratory experiment, C. m. ssp. michiganensis could not survive for 24 h in nutrient solutions with a pH of 4´0 or 4´5, while 1, 14, 51 and 62% of inoculum survived at pH 5´0, 5´5, 6´0 and 6´5, respectively. When tomato plants were inoculated with C. m. ssp. michiganensis through wounds on the stems, the bacteria moved downward from the inoculation site to the roots and infectious bacteria were released from the roots into the nutrient solution. Of two pH regimes tested in greenhouse nutrient-film technique (NFT) culture, the C. m. ssp. michiganensis population was significantly lower in pH 5´0 than in pH 6´5 in most sampling data. In treatments in which C. m. ssp. michiganensis was introduced by transplanting two root-inoculated plants, significantly more plants developed canker at pH 6´5 (34 out of 48 plants) than at pH 5´0 (11 out of 48 plants). When the bacterium was introduced by transplanting two stem-inoculated plants at pH 6´5, seven out of 24 plants developed canker. The potential of pH manipulation in controlling tomato bacterial canker in hydroponic culture is discussed.

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Seed Treatments for EradicatingClavibacter michiganensissubsp.michiganensisfrom Naturally Infected Tomato Seeds

Cited by 49 publications

References 0 publications

Clavibacter michiganensis subsp. michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato

Clavibacter michiganensis subsp. michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato

Avaliação de diferentes tratamentos térmicos no controle do raquitismo-da-soqueira em cana-de-açúcar

Effects of nutrient solution pH on the survival and transmission of Clavibacter michiganensis ssp. michiganensis in hydroponically grown tomatoes

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