Seed treatment and pre-inoculation of soybean: effect of storage period and agrochemicals on the physiological quality of seed and yield

Sandini, Itacir Elói; Belani, Rafael Brugnera; Falbo, Margarete Kimie; Pacentchuk, Fabiano; Huzar‐Novakowiski, Jaqueline

doi:10.5897/ajar2018.13687

Cited by 3 publications

(1 citation statement)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sandini et al [43] indicated that some combinations of fungicides and insecticides may adversely affect the physiological quality of seeds stored for up to 51 days before sowing, but none of them questioned the formation of nodules involving B. elkanii and soybean yield in field conditions.…”

Section: Discussionmentioning

confidence: 99%

Viability of Nodule Bacteria Bradyrhizobium japonicummon Soybean Seeds by Treatment With Fungicides During Extended Storage

Vorobey,

Kukol,

Kots

et al. 2023

Innov Biosyst Bioeng

View full text Add to dashboard Cite

Background. With the increase in soybean cultivation areas, inoculants are becoming increasingly sought after. They are not only compatible with the original preparations for seed treatment of soybean but also offer the possibility of applying them for several days or even months before sowing into the soil. Objective. The viability of new strains of nodule bacteria Bradyrhizobium japonicum (strain PC07 and strain B78) was investigated on the surface of soybean seeds treated with fungicides Fever and Maxim XL during the extended storage of inoculated seeds. Additionally, their ability for nitrogen fixation under symbiotic conditions was evaluated. Methods. Сultivation, serial dilution method, determination of bacterial titer of inoculants, quantification of colony forming units (CFU), gas chromatography. Results. In laboratory conditions, on the soybean seeds of Almaz and Vasylkivska varieties, the viability of the nodule bacteria B. japonicum PC07 and B78, which are more resistant to the active substances of these fungicides in pure culture, decreased. This reduction was dependent on the storage period, the fungicide used, and the strain of inoculant. One day after inoculation on the seeds without the use of fungicide, 68.6–75.4% CFU of the initial number was retained. Seeds treated with the fungicide Fever contained 36.5–38.3% CFU, and those treated with Maxim XL contained 26.2–27.0% CFU. The use of inoculants based on fungicide-resistant strains of B. japonicum PC07 and B78 also provided high cell viability – 2.41–2.8×106 (on the seeds without treatment), 1.40–1.70×106 (with Fever) and 0.8–1.17×106 (with Maxim XL) CFU/seed after 5 days of storage. The nitrogen-fixing activity of root nodules in soybean plants of Almaz and Vasylkivska varieties, whose seeds were treated with Fever, decreased on both varieties by 18.4–22.4% and 32.1–39.5%, respectively and for treatment with Maxim XL, the reduction was 24.5–33.7% and 47.7–75.2%, respectively, during the storage of seeds for 5 and 7 days before sowing, compared to control variants (seeds without fungicide treatment). Conclusions. The utilization of fungicide-resistant strains of B. japonicum PC07 and B78 for bacterization of soybean seeds treated with fungicides Fever and Maxim XL provides a high inoculation titer during 5 days of storage. Viability of microbial cells on seeds treated with fungicides significantly diminishes during storage for more than 7–14 days. Insufficient titer of CFU of B. japonicum strains PC07 and B78 on the seed surface after 14 days of storage can considerably impede the efficacy of biopreparations. Therefore, it is crucial to seek substances that can enhance the resistance of rhizobia on seeds to the adverse effects of fungicide seed treatment, ensuring a longer period of their viability.

show abstract

Section: Discussionmentioning

confidence: 99%

Viability of Nodule Bacteria Bradyrhizobium japonicummon Soybean Seeds by Treatment With Fungicides During Extended Storage

Vorobey,

Kukol,

Kots

et al. 2023

Innov Biosyst Bioeng

View full text Add to dashboard Cite

show abstract

Efficiency of alfalfa seed priming and rhizobial-based liquid inoculants in an Eutric Cambisol

Buntić

Stajković-Srbinović

Knežević

et al. 2021

Zemdirbyste-Agriculture

View full text Add to dashboard Cite

In the last few decades, peat-based bioinoculants have been proven as successful in alfalfa (Medicago sativa L.) seeds inoculation. However, the use of liquid inoculants or pre-inoculated seeds will greatly facilitate the sowing of alfalfa as a small seed legume. Therefore, this study evaluated the effect of four liquid formulations on the survival of Sinorhizobium (Ensifer) meliloti strain 218 during long-term storage. Sodium-alginate (SA) and polyvinylpyrrolidone (PVP) did not affect the growth of S. meliloti strain 218 and showed a significant positive effect on its survival in liquid bioinoculants up to 240 days of storage. The effect of seeds pre-inoculation on the efficiency of nitrogen (N) fixation in alfalfa plants in test tube and a two-year field experiment was measured. The field experiment was conducted in 2018 and 2019 in an Eutric Cambisol. The dry weight of alfalfa shoots and total N content significantly increased in pre-inoculated seeds with all media, compared to the control treatments without pre-inoculation and adding mineral N. In both years of cultivation, the highest total shoot dry weight was achieved by seeds pre-inoculation with SA and PVP 3 months before sowing. Furthermore, in these treatments, shoot dry weight significantly increased compared to the treatment with mineral N in the 2 nd (2019) year. The results of the research showed that rhizobial bioinoculants with SA and PVP could be used as liquid bioinoculants as well as for successful pre-inoculation of alfalfa seeds.

show abstract

Efeito da co-inoculação de bactérias promotoras de crescimento na cultura da soja

Pacentchuk

Gomes

Lima

et al. 2020

RSD

View full text Add to dashboard Cite

A cultura da soja, apresenta com frequência limitações de produtividade, muitas das quais são oriundas de problemas nutricionais, sobretudo pelo fornecimento insuficiente de N. Umas das maneiras para sobrepujar esse problema é por meio de co-inoculação com bactérias promotoras do crescimento de plantas (BPCP), de modo a potencializar a FBN e facilitar a aquisição de N e de outros nutrientes no solo. Assim, o objetivo deste estudo foi avaliar o efeito da co-inoculação, entre B. japonicum e BPCP’s, na produtividade, na nodulação e nos componentes de rendimento da cultura da soja. Foram realizados quatro experimentos na safra 2017/18, os ensaios foram realizados em DBC no esquema fatorial 4 x 12, sendo: quatro (4) locais (Guarapuava/PR, Lapa/PR, Santa Maria do Oeste/PR e Sertão/RS) e 12 tratamentos (uso de BPCP’s associadas com a inoculação padrão). Foi avaliada a produtividade, a nodulação e os componentes de rendimento da cultura da soja. Os resultados obtidos, ainda que positivos na sua maioria, foram variáveis em função de cada local, a variabilidade dos resultados pode ser diminuída por meio do uso de consórcios bacterianos. As BPCP’s proporcionaram incrementos na massa e no número de nódulos, massa de mil grãos, número de grãos por planta, e essas características estão diretamente relacionadas com a produtividade da cultura. Foi observado que as BPCP’s, em co-inoculação com o B. japonicum, sobretudo do gênero Bacillus, propiciaram incrementos de produtividade na cultura da soja, logo se mostraram como importante alternativa para serem utilizadas em co-inoculação com B. japonicum na cultura da soja.

show abstract

Seed treatment and pre-inoculation of soybean: effect of storage period and agrochemicals on the physiological quality of seed and yield

Cited by 3 publications

References 32 publications

Viability of Nodule Bacteria Bradyrhizobium japonicummon Soybean Seeds by Treatment With Fungicides During Extended Storage

Viability of Nodule Bacteria Bradyrhizobium japonicummon Soybean Seeds by Treatment With Fungicides During Extended Storage

Efficiency of alfalfa seed priming and rhizobial-based liquid inoculants in an Eutric Cambisol

Efeito da co-inoculação de bactérias promotoras de crescimento na cultura da soja

Contact Info

Product

Resources

About