Seed transmission of Papaya meleira virus in papaya (<i>Carica papaya</i>) cv. Maradol

Tapia-Tussell, Raúl; Magaña-Álvarez, Anuar; Cortés‐Velázquez, Alberto; Itza‐Kuk, G.; Nexticapan-Garcéz, Ángel; Quijano-Ramayo, Andrés; Martín-Mex, Rodolfo; Pérez‐Brito, Daisy

doi:10.1111/ppa.12279

Cited by 16 publications

(5 citation statements)

References 11 publications

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“…Uma correlação positiva foi observada entre a temperatura e precipitação na ocorrência da doença (MAGAÑA-ALVAREZ et al, 2013). O aparecimento inicial da incidência da doença pode variar de acordo com a fonte de inóculo, a partir de sementes ou hospedeiros alternativos e insetos vetores, que desempenha um papel importante na qualidade sanitária das mudas de mamoeiro TAPIA-TUSSELL et al, 2015). Outro aspecto importante na epidemiologia de doenças de plantas são os hospedeiros alternativos.…”

Section: Página | 129unclassified

ANAIS DO VIII SIMPÓSIO DO PAPAYA BRASILEIRO - Papaya Brasil: Produção e Sustentabildade

2022

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https://editora.incaper.es.gov.br/ Anais do VIII Simpósio do Papaya Brasileiro APRESENTAÇÃO O Brasil se destaca, no cenário mundial, entre os maiores produtores e exportadores de mamão. Porém, apesar de todo esse referencial positivo, fazem-se necessários contínuos investimentos em pesquisas, sobretudo nos aspectos de manejo cultural, água, nutrição, melhoramento genético, fitossanidade e outros; pois esses fatores influenciam diretamente na produtividade e qualidade da fruta, levando em consideração a demanda crescente da sustentabilidade dos sistemas produtivos. O conhecimento para aumentar a produtividade das lavouras, a qualidade, a conservação dos frutos e a segurança do alimento é gerado nas diversas Instituições de Ensino, Pesquisa, Desenvolvimento e Inovação. Em seguida, deve ser difundido no meio científico e repassado para o setor produtivo e de comercialização/exportação para ser adequadamente incorporado às Boas Práticas Agrícolas (BPAs) utilizadas na produção e na pós-colheita do mamão. O Papaya Brasil -Simpósio do Papaya Brasileiroé o principal fórum de atualização e intercâmbio técnico-científico que integra os agentes da cadeia produtiva do mamão, representados pelas Instituições de Ensino, Pesquisa, Extensão e segmentos da comercialização e da exportação. O principal objetivo desse evento é promover a troca de conhecimento científico-tecnológico e de mercado entre todos os integrantes da cadeia do agronegócio dessa fruta. O Papaya Brasil 2022, em sua oitava edição, foi organizado e realizado pelo Centro de Desenvolvimento do Agronegócio (Cedagro) e pelo Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural (Incaper), em conjunto com a Associação Brasileira dos Produtores e Exportadores de Papaya (Brapex) e outros parceiros. Evento realizado no período de 20 a 23 de setembro de 2022, na cidade de Linhares, ES, cujo município é um dos integrantes do maior polo de produção e exportação de mamão no Brasil.O simpósio tem como tema central a Produção e Sustentabilidade do Mamão no Brasil, e também são abordadas questões atuais ligadas ao agronegócio do mamão e relacionadas à sua comercialização e aos fatores que interferem nos processos de sua produção, colheita e pós-colheita, bem como os mais recentes resultados de pesquisas científicas, obtidos por diversas instituições brasileiras de Ciência, Tecnologia e Inovação. Além disso, esse setor produtivo/exportador, apresenta os gargalos e demandas da cultura para servir como indicativo para futuras ações de pesquisa e desenvolvimento. Na visita técnica ao Polo de Produção e Exportação de Mamão de Linhares, região norte do Estado do Espírito Santo, foram apresentadas as BPAs de campo, assim como do processamento dos frutos (packing house), em uma das maiores empresas do setor no Brasil.Esta publicação sintetiza, com êxito, os esforços despendidos na realização do Papaya Brasil 2022, possibilitando que as informações e os resultados apresentados no simpósio, se tornem acessíveis e de fácil consulta para os interessados dos diversas instituiç...

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Section: Página | 129unclassified

ANAIS DO VIII SIMPÓSIO DO PAPAYA BRASILEIRO - Papaya Brasil: Produção e Sustentabildade

2022

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“…In general, the first symptoms of the sticky disease appeared in plants that were 6 to 9 months old, depending upon the papaya variety. The initial onset of the incidence of sticky disease varied according to the source of inoculum, from seeds or alternative hosts and vectors, which played an important role in the quality of the papaya seedlings’ health [ 9 , 23 ].…”

Section: Epidemiologymentioning

confidence: 99%

“…However, another study conducted with seeds from C. papaya cultivar Maradol demonstrated that PMeV is seed-borne and that it can be transmitted to the next generation via contaminated seeds. Furthermore, this finding may explain how this virus appeared in Mexico [ 23 ]. The frequency of virus transmission by seeds can vary greatly depending on the virus-host interaction, and may be very small in some cases [ 25 , 26 ].…”

Section: Epidemiologymentioning

confidence: 99%

“…Weekly inspections are performed in the entire culture crop and plants with symptoms similar to those caused by PMeV are removed. In both countries, with the goals of reducing sticky disease dissemination as well as the prevention or the delaying of its introduction into areas where the disease has not been noted, the following measures have also been recommended: (i) to conduct weekly inspections in orchards and eliminate diseased plants (roguing) as soon as the first symptoms of the sticky disease are detected [ 7 , 9 ]; (ii) to install nurseries and new orchards as far as possible from other orchards, especially those with a history of sticky disease; (iii) not to collect seeds from sticky diseased plants and orchards with PMeV occurrence [ 23 ]; (iv) to try to reduce injuries to plants during cultural treatments and disinfect all material used in the process of thinning and harvesting fruits; (v) to manage low-growing vegetation and keep rows clean and the area between rows trimmed to diminish the variety of weed species; (vi) to eliminate all orchards (diseased and healthy) at the end of the economic cycle of production to eliminate sources of inoculum; (vii) to destroy abandoned orchards, mainly those with plants infected by PMeV as one diseased papaya tree can infect the entire orchard and (viii) to implement crop rotation in papaya producing areas.…”

Section: Disease Managementmentioning

confidence: 99%

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A Current Overview of the Papaya meleira virus, an Unusual Plant Virus

Abreu

Antunes

Magaña-Álvarez

et al. 2015

Viruses

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Papaya meleira virus (PMeV) is the causal agent of papaya sticky disease, which is characterized by a spontaneous exudation of fluid and aqueous latex from the papaya fruit and leaves. The latex oxidizes after atmospheric exposure, resulting in a sticky feature on the fruit from which the name of the disease originates. PMeV is an isometric virus particle with a double-stranded RNA (dsRNA) genome of approximately 12 Kb. Unusual for a plant virus, PMeV particles are localized on and linked to the polymers present in the latex. The ability of the PMeV to inhabit such a hostile environment demonstrates an intriguing interaction of the virus with the papaya. A hypersensitivity response is triggered against PMeV infection, and there is a reduction in the proteolytic activity of papaya latex during sticky disease. In papaya leaf tissues, stress responsive proteins, mostly calreticulin and proteasome-related proteins, are up regulated and proteins related to metabolism are down-regulated. Additionally, PMeV modifies the transcription of several miRNAs involved in the modulation of genes related to the ubiquitin-proteasome system. Until now, no PMeV resistant papaya genotype has been identified and roguing is the only viral control strategy available. However, a single inoculation of papaya plants with PMeV dsRNA delayed the progress of viral infection.

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“…The presence of a viral dsRNA in diseased plants was confirmed, and it was possible to transmit the disease through the latex of infected plants to healthy plants [ 3 ] and also by seeds in cv. Maradol [ 11 ]. However, a cDNA library generated from papaya latex infected by a Mexican isolate (named PMeV-Mx) identified a 1154-bp sequence encoding a putative RdRp showing closer similarity to umbraviruses and no similarity to PMeV-RN.…”

Section: Introductionmentioning

confidence: 99%

The dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease

et al. 2016

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Papaya sticky disease, or “meleira”, is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2.

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Seed transmission of Papaya meleira virus in papaya (Carica papaya) cv. Maradol

Cited by 16 publications

References 11 publications

ANAIS DO VIII SIMPÓSIO DO PAPAYA BRASILEIRO - Papaya Brasil: Produção e Sustentabildade

ANAIS DO VIII SIMPÓSIO DO PAPAYA BRASILEIRO - Papaya Brasil: Produção e Sustentabildade

A Current Overview of the Papaya meleira virus, an Unusual Plant Virus

The dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease

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