Seed Transmission of Como and Potyviruses in Fababean and Vetch Cultivars Introduced into Slovakia

Mali, V. R.; Šubr, Z; Kúdela, O.

doi:10.1556/aphyt.38.2003.1-2.11

Cited by 12 publications

(2 citation statements)

References 18 publications

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“…We found Kahnooj isolate from Iran is closer than other isolates even from Iraq with 93.8 % similarity. It is highly expected that Najaf and Kahnooj isolates are sharing the same ancestor and most likely the virus was imported into Iraq from Iran by international trading (Mali et al, 2003;Gibbs et al, 2008;Al-Kuwaiti 2013).…”

Section: Discussionmentioning

confidence: 99%

Molecular diagnosis of new isolate of tomato yellow leaf curl virus in Iraq

Alabde

Alisawi²,

F³

2021

Preprint

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Tomato yield and quality in Iraq have been threatened by a variable range of infections caused by tomato yellow leaf curl virus. In previous studies, the TYLCV isolates were characterized using molecular tests for small fragments not the entire length of the virus. As a part of long term study, sample of slightly TYLCV-infected tomato has applied in this study to figure out complete sequence of TYLCV isolate. Three sets of primers that belong to three well-known isolates in Iraq were used in a PCR technique and interestingly the results were negative. A new Iraqi isolate has been characterized as a first novel Iraqi isolate detected ever using next generation (NGS) and bioinformatics techniques. The NGS platform has produced about 78,232,062 paired reads of the TYLCV-infected tomato var. Oula F1. The complete raw reads of the infected variety have been analyzed using RepeatExplorer pipeline and Map to reference. The full sequence of TYLCV was reconstructed and extracted to consist of 2770 nt and then deposited in Genbank under accession number MT583814. Copy numbers and genome proportion of this sequence have been calculated that were 3523 and 0,086% respectively. Phylogenetic analyses of full nucleotide sequences confirmed close relationship to Iranian isolate (TYLCV-Kahnooj) than other published viruses. Additionally, a and β DNA satellites have not discovered in the TYLCV-infected sample.

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Section: Discussionmentioning

confidence: 99%

Molecular diagnosis of new isolate of tomato yellow leaf curl virus in Iraq

Alabde

Alisawi²,

F³

2021

Preprint

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“…Interestingly, the closest relative of FA40 is the HG970866 isolate from Australia [9]. Therefore, the phylogenetic analysis showed the absence of geographical-or host-based clustering of BYMV, indicating the long evolutionary history of the virus and its effective dissemination within regions facilitated by hardly controlled seed transmission [34,35].…”

Section: Genome Characterization Of Slovak Bymv Isolatesmentioning

confidence: 97%

Molecular Characteristics and Biological Properties of Bean Yellow Mosaic Virus Isolates from Slovakia

Mrkvová,

Kemenczeiová,

Achs

et al. 2024

Horticulturae

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Analysis of the viromes of three symptomatic Fabaceae plants, i.e., red clover (Trifolium pratense L.), pea (Pisum sativum L.), and common bean (Phaseolus vulgaris L.), using high-throughput sequencing revealed complex infections and enabled the acquisition of complete genomes of a potyvirus, bean yellow mosaic virus (BYMV). Based on phylogenetic analysis, the Slovak BYMV isolates belong to two distinct molecular groups, i.e., VI (isolate FA40) and XI (isolates DAT, PS2). Five commercial pea genotypes (Alderman, Ambrosia, Gloriosa, Herkules, Senator) were successfully infected with the BYMV-PS2 inoculum and displayed similar systemic chlorotic mottling symptoms. Relative comparison of optical density values using semi-quantitative DAS-ELISA revealed significant differences among virus titers in one of the infected pea genotypes (Ambrosia) when upper fully developed leaves were tested. Immunoblot analysis of systemically infected Alderman plants showed rather uneven virus accumulation in different plant parts. The lowest virus accumulation was repeatedly detected in the roots, while the highest was in the upper part of the plant stem.

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Introduction

Sastry¹

2012

Seed-Borne Plant Virus Diseases

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Seed Transmission of Como and Potyviruses in Fababean and Vetch Cultivars Introduced into Slovakia

Cited by 12 publications

References 18 publications

Molecular diagnosis of new isolate of tomato yellow leaf curl virus in Iraq

Molecular diagnosis of new isolate of tomato yellow leaf curl virus in Iraq

Molecular Characteristics and Biological Properties of Bean Yellow Mosaic Virus Isolates from Slovakia

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