Seed-to-seed growth of Arabidopsis Thaliana on the international space station

Link, Bruce M.; Durst, S.; Zhou, Weijia; Stanković, Bratislav

doi:10.1016/s0273-1177(03)00250-3

Cited by 66 publications

(53 citation statements)

References 7 publications

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“…Space experiments have also been plagued by high ethylene levels. In this experiment, ethylene levels were kept below 100 ppb by using a photocatalytic converter to remove ethylene from the growth chamber (Zhou et al, 2002;Link et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…In a partnership agreement, WCSAR and Space Explorers, Inc., an educational company in Green Bay, Wisconsin, grew Arabidopsis thaliana in the ADVASC unit on the ISS. ADVASC represented a substantial advance in plant growth facilities in that it was fully automated, required very little care from astronauts, and was remotely controlled from the ground (Zhou et al, 2002;Link et al, 2003). The first flight of ADVASC provided an opportunity to study the patterns of plant growth and development as well as seed and plant morphology in microgravity (first seed-to-seed Arabidopsis experiment on the ISS).…”

Section: Introductionmentioning

confidence: 99%

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Seed-to-Seed-to-Seed Growth and Development of Arabidopsis in Microgravity

Link

Busse²,

Stanković³

2014

Astrobiology

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Arabidopsis thaliana was grown from seed to seed wholly in microgravity on the International Space Station. Arabidopsis plants were germinated, grown, and maintained inside a growth chamber prior to returning to Earth. Some of these seeds were used in a subsequent experiment to successfully produce a second (back-toback) generation of microgravity-grown Arabidopsis. In general, plant growth and development in microgravity proceeded similarly to those of the ground controls, which were grown in an identical chamber. Morphologically, the most striking feature of space-grown Arabidopsis was that the secondary inflorescence branches and siliques formed nearly perpendicular angles to the inflorescence stems. The branches grew out perpendicularly to the main inflorescence stem, indicating that gravity was the key determinant of branch and silique angle and that light had either no role or a secondary role in Arabidopsis branch and silique orientation. Seed protein bodies were 55% smaller in space seed than in controls, but protein assays showed only a 9% reduction in seed protein content. Germination rates for space-produced seed were 92%, indicating that the seeds developed in microgravity were healthy and viable. Gravity is not necessary for seed-to-seed growth of plants, though it plays a direct role in plant form and may influence seed reserves.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Seed-to-Seed-to-Seed Growth and Development of Arabidopsis in Microgravity

Link

Busse²,

Stanković³

2014

Astrobiology

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“…Some of the seeds that were harvested from the plants that were grown in microgravity were planted in a ground study. These seeds produced typical plants without any visible abnormalities [Link et al, 2003]. Soybeans were also grown from seed to seed for the first time in space.…”

Section: Fundamentals Of Space Medicinementioning

confidence: 99%

Fundamentals of Space Medicine

Clément

2011

140

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“…Systems have been incorporated into both temporarily and permanently installed ISS plant growth hardware such as Advanced Astroculture (Link et al, 2003;Zhou, 2005), Biomass Production System (BPS) (Morrow and Crabb, 2000;Stutte et al, 2005), Plant Generic Bioprocessing Apparatus (PGBA) (Hoehn et al, 1996;Evans et al, 2009), Lada (Sychev et al, 2007;Bingham et al, 2003), European Modular Cultivation System (EMCS) (Brinckmann, 2005;Johnsson et al, 2009), Biolab (Brinckmann, 2005) and the Green Fluorescent Protein (GFP) Imaging System (GIS) within the Advanced Biological Research System (ABRS) (Paul et al, 2012;Levine et al, 2009). Visible light imaging has been implemented in the bulk of these plant growth chambers, but monitoring has also been conducted through infrared and fluorescent means.…”

Section: Tablementioning

confidence: 99%

Flexible imaging payload for real-time fluorescent biological imaging in parabolic, suborbital and space analog environments

Bamsey

Paul

Graham

et al. 2014

Life Sciences in Space Research

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Fluorescent imaging offers the ability to monitor biological functions, in this case biological responses to space-related environments. For plants, fluorescent imaging can include general health indicators such as chlorophyll fluorescence as well as specific metabolic indicators such as engineered fluorescent reporters. This paper describes the Flex Imager a fluorescent imaging payload designed for Middeck Locker deployment and now tested on multiple flight and flight-related platforms. The Flex Imager and associated payload elements have been developed with a focus on 'flexibility' allowing for multiple imaging modalities and change-out of individual imaging or control components in the field. The imaging platform is contained within the standard Middeck Locker spaceflight form factor, with components affixed to a baseplate that permits easy rearrangement and fine adjustment of components. The Flex Imager utilizes standard software packages to simplify operation, operator training, and evaluation by flight provider flight test engineers, or by researchers processing the raw data. Images are obtained using a commercial cooled CCD image sensor, with light-emitting diodes for excitation and a suite of filters that allow biological samples to be imaged over wavelength bands of interest. Although baselined for the monitoring of green fluorescent protein and chlorophyll fluorescence from Arabidopsis samples, the Flex Imager payload permits imaging of any biological sample contained within a standard 10 cm by 10 cm square Petri plate. A sample holder was developed to secure sample plates under different flight profiles while permitting sample change-out should crewed operations be possible. In addition to crewdirected imaging, autonomous or telemetric operation of the payload is also a viable operational mode. An infrared camera has also been integrated into the Flex Imager payload to allow concurrent fluorescent and thermal imaging of samples. The Flex Imager has been utilized to assess, in real-time, the response of plants to novel environments including various spaceflight analogs, including several parabolic flight environments as well as hypobaric plant growth chambers. Basic performance results obtained under these operational environments, as well as laboratory-based tests are described. The Flex Imager has also been designed to be compatible with emerging suborbital platforms.

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Seed-to-seed growth of Arabidopsis Thaliana on the international space station

Cited by 66 publications

References 7 publications

Seed-to-Seed-to-Seed Growth and Development of Arabidopsis in Microgravity

Seed-to-Seed-to-Seed Growth and Development of Arabidopsis in Microgravity

Fundamentals of Space Medicine

Flexible imaging payload for real-time fluorescent biological imaging in parabolic, suborbital and space analog environments

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