Seed storage protein gene promoters contain conserved DNA motifs in Brassicaceae, Fabaceae and Poaceae

Fauteux, François; Strömvik, Martina V.

doi:10.1186/1471-2229-9-126

Cited by 50 publications

(41 citation statements)

References 83 publications

(71 reference statements)

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“…These results were consistent with those of the GUS activity in transgenic Arabidopsis seed. Fauteux and Strmvik (2009) reported that seed storage protein gene promoters contain conserved DNA motifs (two RY-like and one ACGT-like) in the Brassicaceae, Fabaceae, and Poaceae. In Phaseolus vulgaris, several conserved RY-elements of the DLEC2 promoter (Bobb et al, 1997) and phaseolin promoter (Chandrasekharan et al, 2003) were necessary for seed-specific expression during embryogenesis.…”

Section: Resultsmentioning

confidence: 99%

Isolation and functional characterization of the Brassica napus cruciferin gene cru4 promoter

Roh

Choi

Kang

et al. 2014

J Korean Soc Appl Biol Chem

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The 12S globulin protein cruciferin is main seed storage protein in Brassica napus. To gain a better understanding of the Bncru4 promoter function, we conducted the promoter 5' deletion analysis in transgenic Arabidopsis. In the β-glucuronidase (GUS) expression assay, Bncru4 promoter was strongly active in transgenic seeds. In addition, deletion of RY-elements (−236 bp region) dramatically decreased the promoter activity in seed embryos; however, the GUS expression could be observed in seed coat. Further deletion up to −113 bp region (removed up to the CAAT and TATA box), GUS expression was completely abolished in all tissues. These results were consistent with that of the GUS activity in transgenic seeds. Therefore, we consider that RYelement is crucial to the seed-specific expression of Bncru4 promoter.

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Section: Resultsmentioning

confidence: 99%

Isolation and functional characterization of the Brassica napus cruciferin gene cru4 promoter

Roh

Choi

Kang

et al. 2014

J Korean Soc Appl Biol Chem

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“…Deletion of the region containing these motifs from the napA promoter decreased GUS expression in transgenic tobacco plants (Stalberg et al, 1993). A RY motif (CATGCA / CACGT) is commonly found in seed storage protein genes (Fauteux and Stromvik, 2009), including Ara h 1 (Viquez et al, 2003) and Ara h 2 (this study), and acts by enhancing seed specific gene expression (Dickinson et al, 1988). Certain regulatory elements are fundamental to growth regulator signaling pathways which are important in seed development.…”

Section: Discussionmentioning

confidence: 99%

Reporter Gene Expression Patterns Regulated by an Ara h 2 Promoter Differ in Homologous Versus Heterologous Systems1

Bhattacharya¹,

Ramos²,

Faustinelli

et al. 2012

Peanut Science

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Peanut (Arachis hypogaea L.) is a globally important crop whose seeds are widely used in food products. Peanut seeds contain proteins that serve a nutrient reservoir function and that also are major allergens. As part of an investigation to determine the effect of reducing/ eliminating the peanut allergen Ara h 2 from seeds, gene sequence including upstream regulatory regions was characterized. The ability of regions upstream of the translation initiation site to regulate seed-specific expression of reporter genes was tested in peanut and Arabidopsis. Two independent transgenic peanut lines biolistically transformed with 1kb of DNA upstream of the Ara h 2.02 (B-genome) coding sequence controlling a Green Fluorescent Protein -b-glucuronidase (Gfp-Gus) fusion were obtained. All T 1 , T 2 and T 3 generations of transgenic plants showed the expression of GFP and GUS restricted to seeds and near background levels in vegetative tissues. However, constitutive GUS expression was observed in Arabidopsis transgenic lines, a heterologous system. It is possible that transacting factors regulating seed specificity in peanut are too divergent in Arabidopsis to enable the seed specific response. Thus, the promoter described in this paper may have potential use for expression of transgenes in peanut where seed-specificity is desired, but expression patterns should be tested in heterologous systems prior to off-the-shelf adoption.

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“…Promoter sequences are often conserved between diverse plant species (Fauteux and Strömvik 2009); (Koch et al 2001) that allow the use of A. thaliana as reference to perform TFBS analysis. Upstream sequences of orthologous Arabidopsis genes, corresponding to 9676 transcripts, were processed to identify TFBS.…”

Section: Identification Of Transcription Factor Binding Sitesmentioning

confidence: 99%

Computational Analysis of “-omics” Data to Identify Transcription Factors Regulating Secondary Metabolism in Rauvolfia serpentina

Pathania

Acharya

2015

Plant Mol Biol Rep

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Rauvolfia serpentina has been known to produce therapeutically important indole alkaloids used in treatment of various diseases. Despite its medicinal importance, complete understanding of its secondary metabolism is challenging due to complex interplay among various transcription factors (TFs) and genes. However, weighted co-expression analysis of transcriptome along with integration of metabolomics data has proficiency to elucidate topological properties of complex regulatory interactions in secondary metabolism. We aimed to implement an integrative strategy using B-omicsd ata to identify TFs of Bunknown function^and exemplify their role in regulation of valuable metabolites as well as metabolic traits. A total of 69 TFs were identified through significant thresholds and removal of false positives based on cisregulatory motif analysis. Network-biology inspired analysis of co-expression network lead to generation of four statistically significant and biologically robust modules. Similar to known regulatory roles of WRKY and AP2-EREBP TF families in Catharanthus roseus, this study presented them to regulate synthesis of alkaloids in R. serpentina as well.Moreover, TFs in module 4 were observed to be regulating connecting steps between primary and secondary metabolic pathways in the synthesis of terpenoid indole alkaloids. Integration of metabolomics data further highlight the significance of module 1 since it was statistically predicted to be involved in synthesis of specialized metabolites, and associated genes may physically clustered on genome. Importantly, putative TFs in module 1 may modulate the major indole alkaloids synthesis in response to various environmental stimuli. The methodology implemented herein may provide a better reference to identify and explore functions of transcriptional regulators.

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Seed storage protein gene promoters contain conserved DNA motifs in Brassicaceae, Fabaceae and Poaceae

Cited by 50 publications

References 83 publications

Isolation and functional characterization of the Brassica napus cruciferin gene cru4 promoter

Isolation and functional characterization of the Brassica napus cruciferin gene cru4 promoter

Reporter Gene Expression Patterns Regulated by an Ara h 2 Promoter Differ in Homologous Versus Heterologous Systems1

Computational Analysis of “-omics” Data to Identify Transcription Factors Regulating Secondary Metabolism in Rauvolfia serpentina

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