Seed storage protein electrophoretic profiles in some Iranian date palm (Phoenix dactylifera L.) cultivars

Khoshroo, Sayed Mohammad Reza

doi:10.5897/ajb11.2726

Cited by 10 publications

(6 citation statements)

References 36 publications

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“…However, the band was not observed in SDS-PAGE under reducing conditions, indicating that the reduced fragments of protein by mercaptoethanol could be out of range of the used protein marker (10-180 kDa). Khoshroo et al [55] studied the protein profile of twelve different date seed varieties grown in Iran. They found one heavily stained band at around 65 kDa and minor bands ranging from 12 to 369 kDa using by SDS-PAGE technique.…”

Section: Comparison Of Physicochemical Properties Of Dspcmentioning

confidence: 99%

“…However, the band was not observed in SDS-PAGE under reducing conditions, indicating that the reduced fragments of protein by mercaptoethanol could be out of range of the used protein marker (10-180 kDa). Khoshroo et al [55] studied the protein profile of twelve different date seed varieties grown in Iran. They found one heavily stained band FTIR, a meaningful way to characterize the secondary structure of proteins by analyzing the vibrational states of the chemical bonds in the proteins, has been used to investigate the effect of ultrasound on the structure of DSPC [58].…”

Section: Comparison Of Physicochemical Properties Of Dspcmentioning

confidence: 99%

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Improving the Functional Performance of Date Seed Protein Concentrate by High-Intensity Ultrasonic Treatment

Kelany

Yemiş

2022

Molecules

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Date kernel is a plant-derived byproduct that has the potential to be converted into a high-value-added food ingredient, such as protein concentrate, in the food industry. Ultrasound, which is an alternative method for improving the functional properties of food proteins, is an effective physical treatment for modifying protein functionality. Solubility is the main criterion that primarily affects other functional properties of protein concentrates, such as emulsification, foaming, and water and oil binding. The aim of this study is to enhance the techno-functional performance of date seed protein concentrate (DSPC) by maximizing the solubility via a high-intensity ultrasound (HIUS) treatment at a fixed frequency of 20 kHz. The effect of ultrasonic homogenization under varying amplitudes and times (amplitude of 40, 60, and 80% for 5, 10, and 15 min, respectively) on the functional properties of the DSPC was investigated by using the response surface methodology (RSM). A face-centered central composite design (FC-CCD) revealed that the optimal process conditions of HIUS were at an amplitude of 80% for 15 min. The physicochemical and functional properties of the ultrasound-applied concentrate (DSPC-US) were determined under the optimum HIUS conditions, and then these properties of DSPC-US were compared to the native DSPC. The results showed that the solubility of all DSPC samples treated by HIUS was significantly (p < 0.05) higher than that of the native DSPC. In addition, emulsion activity/stability, foaming activity/stability, and oil-binding capacity increased after HIUS homogenization treatments, whereas the water-binding capacity decreased. These changes in the techno-functional properties of the DSPC-US were explained by the modification to the physicochemical structure of the DSPC (particle size, zeta potential, SDS-PAGE, SEM, FTIR, DSC, free SH content, surface hydrophobicity, and intrinsic emission). This work revealed that HIUS could be an effective treatment for enhancing the functional properties of date seed protein concentrate.

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Section: Comparison Of Physicochemical Properties Of Dspcmentioning

confidence: 99%

“…However, the band was not observed in SDS-PAGE under reducing conditions, indicating that the reduced fragments of protein by mercaptoethanol could be out of range of the used protein marker (10-180 kDa). Khoshroo et al [55] studied the protein profile of twelve different date seed varieties grown in Iran. They found one heavily stained band FTIR, a meaningful way to characterize the secondary structure of proteins by analyzing the vibrational states of the chemical bonds in the proteins, has been used to investigate the effect of ultrasound on the structure of DSPC [58].…”

Section: Comparison Of Physicochemical Properties Of Dspcmentioning

confidence: 99%

Improving the Functional Performance of Date Seed Protein Concentrate by High-Intensity Ultrasonic Treatment

Kelany

Yemiş

2022

Molecules

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“…2003). Assessment of the genetic diversity among date palm genotypes is performed by morphological parameters (AHMED et al, 2011;NAQVI et al, 2015;SALEM et al, 2008), biochemical (GOMEZ-VIDAL et al, 2008;KHOSHROO et al, 2011;SALEM et al, 2001) or molecular markers (ABDULLA; GAMAL, 2010; AHMED; AL-QARADAWI, 2009;ELSHIBLI;KORPELAINEN, 2008;ZEHDI et al, 2004). Several morphological markers have been used to study the genetic diversity of date palms and discriminate between closely related genotypes (ARABNEZHAD et al, 2012;EL-ASSAR et al, 2005;RACCHI et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Assessment of phenotypic diversity of some date palm male genotypes growing under Egyptian conditions

et al. 2023

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Artificial pollination is a prerequisite to achieve a sufficient yield of palm trees; hence the identification of superior male palm trees as a standard pollen source for date palm growers has a high priority. The current study was carried out to assess phenotypic diversity among four date palm males namely Hayani, Meghal, Fardh, and Ghannami Ahmer. The genetic diversity and phylogenetic relationships between palm genotypes were performed using eighteen quantitative morphological parameters of both vegetative and reproductive parts (leaf, leaflets, spines, and spathe). Principal components analysis and hierarchical clustering was performed to identify the differences and similarities between the studied genotypes. Results showed significant differences in morphological parameters between the four male palm trees. Most of the selected morphological parameters could be used as quantitative markers for the identification of date palm male genotypes. Principal component analysis showed that, the studied parameters formed two major homogeneous groups of variables, which possessed a high proportion of the observed phenotypic diversity among the studied date palm males. Cluster analysis showed that Fardh, Ghannami Ahmer, and Meghal, are closer related than Hayani; Ghannami and Fardh males showed the highest genetic similarity while the lowest similarity index was detected between Hayani and Ghannami Ahmer males.

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“…The fruit contains30 mg of phosphorus, 1.4 g of protein, 0.7 mg of iron, 10 mg of calcium, 2 mg vitamin C, and 50 µg of carotene in 100 g of edible portion [23]. Genetic analysis based on quantitative traits has been made in this crop in Pakistan [24] [25] [26] [27]. However, phenotypic characters have limitations as they are influenced by environmental factors and the developmental stages of the plant.…”

Section: Introductionmentioning

confidence: 99%

Genetic Variation among Seedling of Pumpkins Genotypes through SDS-Page

Ikram¹,

Ali²,

Ishaq³

et al. 2021

AJPS

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Genetic diversity in 30 genotypes of Pumpkins was collected from unexplored mountainous areas of Khyber Pakhtunkhwa, Pakistan was investigated through biochemical characterization. For biochemical characterization, Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis was carried out. The seed proteins were resolved on 7.5% and 15% polyacrylamide gel. A total of 35% genetic disagreement was observed in the collected lines with linkage distances ranging from 0.00 -0.83 (percent disagreement). Similarly, cluster analysis sorted total germplasm on the basis of 12 bands (total bands) into eight clusters. Present study revealed a considerable amount of genetic diversity explored in pumpkin germplasm, Cluster analysis exhibited moderate level of genetic diversity; to broaden the gene pool. Further collection of the important germplasm is needed to be used in the development of improved cultivars with respect to quality and quantity.

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Seed storage protein electrophoretic profiles in some Iranian date palm (Phoenix dactylifera L.) cultivars

Cited by 10 publications

References 36 publications

Improving the Functional Performance of Date Seed Protein Concentrate by High-Intensity Ultrasonic Treatment

Improving the Functional Performance of Date Seed Protein Concentrate by High-Intensity Ultrasonic Treatment

Assessment of phenotypic diversity of some date palm male genotypes growing under Egyptian conditions

Genetic Variation among Seedling of Pumpkins Genotypes through SDS-Page

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