Seed-specific expression of an acyl-acyl carrier protein thioesterase CnFatB3 from coconut ( Cocos nucifera L.) increases the accumulation of medium-chain fatty acids in transgenic Arabidopsis seeds

Yang, Yuan; Gao, Lijuan; Sun, Rong; Yu, Tian; Liang, Yuanxue; Li, Dongdong; Zheng, Yusheng

doi:10.1016/j.scienta.2017.05.029

Cited by 16 publications

(8 citation statements)

References 28 publications

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“…In maize, the palmitic content of mutants with an 11 bp insertion or single nucleotide insertion in FATB were reduced [ 6 , 32 ]. Overexpression of CnFATB3 from coconut in Arabidopsis seeds resulted in the increase in saturated FA content [ 33 ]. Soybean mutant N0304-303-3, which contains a 254-kb genomic deletion containing 19 genes, including the FATB1a gene, have low levels of palmitic acid [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Knockout of GmFATB1 Significantly Reduced the Amount of Saturated Fatty Acids in Soybean Seeds

Sun

Whelan

et al. 2021

IJMS

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Soybean (Glycine max) oil is one of the most widely used vegetable oils across the world. Breeding of soybean to reduce the saturated fatty acid (FA) content, which is linked to cardiovascular disease, would be of great significance for nutritional improvement. Acyl-acyl carrier protein thioesterases (FATs) can release free FAs and acyl-ACP, which ultimately affects the FA profile. In this study, we identified a pair of soybean FATB coding genes, GmFATB1a and GmFATB1b. Mutants that knock out either or both of the GmFATB1 genes were obtained via CRISPR/Cas9. Single mutants, fatb1a and fatb1b, showed a decrease in leaf palmitic and stearic acid contents, ranging from 11% to 21%. The double mutant, fatb1a:1b, had a 42% and 35% decrease in palmitic and stearic acid content, displayed growth defects, and were male sterility. Analysis of the seed oil profile revealed that fatb1a and fatb1b had significant lower palmitic and stearic acid contents, 39–53% and 17–37%, respectively, while that of the unsaturated FAs were the same. The relative content of the beneficial FA, linoleic acid, was increased by 1.3–3.6%. The oil profile changes in these mutants were confirmed for four generations. Overall, our data illustrate that GmFATB1 knockout mutants have great potential in improving the soybean oil quality for human health.

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Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Knockout of GmFATB1 Significantly Reduced the Amount of Saturated Fatty Acids in Soybean Seeds

Sun

Whelan

et al. 2021

IJMS

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“…Compared to that on other important crops, research on coconut has evolved slowly, especially with respect to the transcriptional regulation of fruit and endosperm development. In past research, most of the studies were related to the synthesis and accumulation of medium-chain fatty acids in coconut endosperm (Yuan et al 2017(Yuan et al , 2015Zheng et al 2019). Only one transcription factor related to oil metabolism, WRI1, was cloned and identified from coconut endosperm development (Sun et al 2017b).…”

Section: Discussionmentioning

confidence: 99%

“…Seed oil was extracted from mature seeds derived from each of the transgenic lines and WT seeds of Arabidopsis thaliana using trichloromethane/methanol (2:1), and the relative weight percentage of seed oil was obtained after air-drying the extract (Yuan et al 2017). The extract was transmethylated with 2% KOH-methanol (m/v) at 80 °C for 2 h. The fatty acid methyl esters (FAMEs) were recovered using n-hexane.…”

Section: Analysis Of Lipid and Fatty Acid Contentsmentioning

confidence: 99%

CnMADS1, a MADS transcription factor, positively modulates cell proliferation and lipid metabolism in the endosperm of coconut (Cocos nucifera L.)

Sun

Gao

et al. 2020

Planta

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“…Both the lipid and fatty acid analyses of Arabidopsis plants and oil palm embryoids were conducted according to previous studies ( Yuan et al, 2017 ). In brief, 5 ml chloroform-methanol (volume ratio 2:1) was used to extract lipids, and then, lipids were dried by a Termovap sample concentrator.…”

Section: Methodsmentioning

confidence: 99%

EgmiR5179 Regulates Lipid Metabolism by Targeting EgMADS16 in the Mesocarp of Oil Palm (Elaeis guineensis)

et al. 2021

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EgMADS16, one of the MADS-box transcription factors in oil palm, has a high expression level in the late fruit development of the oil palm fruit mesocarp. At the same time, it is also predicted to be the target gene of EgmiR5179, which has been identified in previous research. In this paper, we focused on the function and regulatory mechanism of the EgMADS16 gene in oil palm lipid metabolism. The results indicated that the transcription level of EgMADS16 was highest in the fourth stage, and a dual-luciferase reporter assay proved that the EgMADS16 expression level was downregulated by EgmiR5179. In both the OXEgMADS16 Arabidopsis seeds and oil palm embryonic calli, the total lipid contents were significantly decreased, but the contents of C18:0 and C18:3 in OXEgMADS16 lines were significantly increased. As expected, EgmiR5179 weakened the inhibitory effect of EgMADS16 on the oil contents in transgenic Arabidopsis plants that coexpressed EgmiR5179 and EgMADS16 (OXEgmiR5179-EgMADS16). Moreover, yeast two-hybrid and BiFC analyses suggested that there was an interaction between the EgMADS16 protein and EgGLO1 protein, which had been proven to be capable of regulating fatty acid synthesis in our previous research work. In summary, a model of the molecular mechanism by which miRNA5179 targets EgMADS16 to regulate oil biosynthesis was hypothesized, and the research results provide new insight into lipid accumulation and molecular regulation in oil palm.

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Seed-specific expression of an acyl-acyl carrier protein thioesterase CnFatB3 from coconut ( Cocos nucifera L.) increases the accumulation of medium-chain fatty acids in transgenic Arabidopsis seeds

Cited by 16 publications

References 28 publications

CRISPR/Cas9-Mediated Knockout of GmFATB1 Significantly Reduced the Amount of Saturated Fatty Acids in Soybean Seeds

CRISPR/Cas9-Mediated Knockout of GmFATB1 Significantly Reduced the Amount of Saturated Fatty Acids in Soybean Seeds

CnMADS1, a MADS transcription factor, positively modulates cell proliferation and lipid metabolism in the endosperm of coconut (Cocos nucifera L.)

EgmiR5179 Regulates Lipid Metabolism by Targeting EgMADS16 in the Mesocarp of Oil Palm (Elaeis guineensis)

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