Seed protein electrophoresis of some members of the family fabaceae

Alege, Gbenga Olorunshola; Abu, Ngozi E.; Sunday, C. E

doi:10.5897/ajb2014.13715

Cited by 14 publications

(21 citation statements)

References 12 publications

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“…Standard deviation was judged non reliable to estimate the intraspecific variation, due to the relatively low and uneven number of values for evaluating intra-specific variation. However, for each documented species, trait and agroecosystem properties have been measured on various genotypes or cultivars, which probably accounts for within species variability 85 . In addition, genera boundary are still discussed in grain legumes 83 .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of pulse crops’ functional diversity supporting food production

Guiguitant

Vile

Ghanem

et al. 2020

Sci Rep

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Pulses, defined as legumes which produce dry seed used for human consumption, are plants of great agronomic value, at the food system level as much as the field level but their diversity has been largely underused. This study aimed at analyzing existing data on cultivated pulse species in the literature to provide a broad and structured description of pulses' interspecific functional diversity. We used a functional trait-based approach to evaluate how pulse diversity could support food production in agroecosystems constrained by low water and nutrient availability and exposed to high weed pressure. We gathered data for 17 functional traits and six agroecosystem properties for 43 pulse species. Our analytical framework highlights the correlations and combinations of functional traits that best predict values of six agroecosystem properties defined as ecosystem services estimates. We show that pulse diversity has been structured both by breeding and by an environmental gradient. The covariance space corresponding to agroecosystem properties was structured by three properties: producers, competitors, stress-tolerant species. The distribution of crop species in this functional space reflected ecological adaptive strategies described in wild species, where the size-related axis of variation is separated from variation of leaf morpho-physiological traits. Six agroecosystem properties were predicted by different combinations of traits. However, we identified ubiquitous plant traits such as leaflet length, days to maturity, seed weight, and leaf nitrogen content, that discriminated agroecosystem properties and allowed us to gather individual species into three clusters, representative of the three strategies highlighted earlier. Implications for pulses provisioning of services in agroecosystems are discussed.Over the past decade, grain legumes used for human consumption -pulses -have been receiving a resurgence of interest to meet agricultural challenges all over the world. Indeed, they are particularly important in human nutrition as sources of proteins, vitamins and minerals that complement a predominantly cereal-based diet 1,2 . At field level, their well-known ability to fix atmospheric nitrogen helps reducing energy consumption while making them particularly suitable for low-input systems. They are also a source of diversification in order to break disease, pest and weed cycles and optimize nutrient management in standard crop rotations 3 . Although site-specific environmental constraints may reduce cropping systems options, the diversity of pulse, when fully explored, may offer solutions to most cropping systems, from a biophysical point of view. Indeed, leguminous plants constitute the third largest family among flowering plants and contain no less than 18,000 species 4 . This diversity implies potentially highly variable responses to abiotic and biotic stresses 5 . However, the specific diversity of pulse is poorly represented in most cropping systems despite the growing demand from foodchains 6 . Most of pulse s...

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Section: Discussionmentioning

confidence: 99%

Evaluation of pulse crops’ functional diversity supporting food production

Guiguitant

Vile

Ghanem

et al. 2020

Sci Rep

View full text Add to dashboard Cite

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“…The hierarchical cluster of studied plants showed two major clusters, the first major cluster grouped into two sub-cluster, each genus under the same sub-cluster indicates close genetic affinity and common ancestry (Alege, 2015), while the second major cluster contained only one genus. The presence of two clusters suggests more than one evolutionary line (Alege et al, 2014). Several researches used hierarchical cluster to detect genetic diversity among plants (Irfan et al, 2007;Alege et al, 2014;Bruneau et al, 2001;Amouri et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…The presence of two clusters suggests more than one evolutionary line (Alege et al, 2014). Several researches used hierarchical cluster to detect genetic diversity among plants (Irfan et al, 2007;Alege et al, 2014;Bruneau et al, 2001;Amouri et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Automated electrophoresis analysis of protein showed different number of bands, and only for two bands, the first common bands numbers were 8,7,8,9,8,12,10, and 11 and the second bands were 14, 10, 14, 14, 13, 13, 12, and 13 among the studied plants. Several researches detected genetic diversity among plants by using electrophoresis technique (Ehsanpour et al, 2010;Sinha et al, 2012;Alege et al, 2014). attributed that the appearance of a common band in individual plants in population is due to the fact that the gene expression of the protein (enzyme) does not vary in these plants, and there is polymorphism on the basis of differences in protein intensity among genotypes (Munazza et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

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Proteins patterns of eight genera of the Asteraceae family

Al-Ahmadi¹

2017

Afr. J. Biotechnol.

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Asteraceae family grows as wild plants in the eastern province of Saudi Arabia near the Gulf coast with prevalent high humidity coupled with more moderate temperatures. Eight genera were collected during the flowering season to study genetic diversity according to protein patterns for each plant. The protein patterns showed different numbers of bands, concentrations, molecular weight and intensity. Two common bands were observed to ensure the existence of fixed gene and it can also be considered as a marker of the studied genera. Protein profiles revealed genetic diversity among these species for adaptation to environmental factors. Similarity coefficient was high for Sonchus oleranceus and Senecio desfontainei, while low for Launaea capitata and Osteospermum vaillantii among the studied plants. The hierarchical cluster analysis, formed two major clusters, which indicates the existence of genetic diversity among the studied genera. The first cluster was divided into two sub-clusters. Subcluster A comprised three species, Scorzonera papposa , Senecio desfontainei , and Sonchus oleranceus; and the second sub-cluster B comprised four species Anthemis melampodina, Echinops hussoni, Launaea capitata, and O. vaillanti. The second cluster contained only one species Artemisia inculta, which suggests that the Asteraceae family may have more than one evolutionary line.

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“…In general, date palms produce inferior fruits when propagated by seeds, therefore offshoots are preferred for conventional propagation, as offshoots produce fruit identical to the mother tree (Abass, 2013;Kenna and Mansfield, 1997). Utilizing DNA markers is most accurate than protein patterns in studying similarities between cultivars, but still protein profiles characterized by high stability and reproducibility, which produces reliable results to verify the differences within and between species and cultivars (Dakhil et al, 2013;Rouholamin and Saei, 2016;Alege et al, 2014;George et al, 2013), electrophoretic analysis of protein bands has been used as an effective tool in recognizing the origin of cultivated plants (Ladizinsky and Hymowitz, 1979). It is a fast and inexpensive tool (Smykal et al, 2008;Rouholamin and Saei, 2016).…”

Section: Introductionmentioning

confidence: 99%

Analysis of similarities within different date palm cultivars of Al-Ahsa oasis in Saudi Arabia

Al-Issa¹

2017

Afr. J. Biotechnol.

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Date palm (Phoenix dactylifera L.) is widely cultivated in Saudi Arabia, with annual production estimated to be above one million tons. Of the 450 cultivars that are commonly used, 70 cultivars are found in the Eastern province and 20 cultivars are well known and common in the Al-Ahsa oasis. In this study, thirty seven date palm samples representing nine cultivars collected from six locations in the AlAhsa oasis, Saudi Arabia. Total proteins were extracted and subjected to analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results revealed several differences within the cultivars. Overall polymorphism ranged between 12 and 76%, with the highest being 76% within Helali cultivar, and the least being 12% within OmRohaim cultivar. These results support the conclusion that some palms are grown from seeds resembling a known cultivar, which are later distributed as the known cultivar. These results revealed differences in the degree of similarity between the cultivars from 0.059 to 0.579, estimated according to Jaccard`s similarity method based on the presence and absence of protein bands. Khosab and Shehel cultivars were closely related to each other with 0.579 degrees of similarity, both separated by phylogenetic dendrogram in mini-cluster, which have been confirmed by the 1st principal components. The mini-cluster was related to OmRohaim and Khonaizi cultivars within a larger cluster. Shishi, Garrah and Helali were related cultivars, with degrees of similarities ranging between 0.250 and 0.455, and were separated in one cluster by phylogenetic dendrogram. The close relationship between the cultivars may indicate that they originated from the same ancestor. Further molecular and biochemical studies are needed to enrich our knowledge about the relationships among date palm cultivars.

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Seed protein electrophoresis of some members of the family fabaceae

Cited by 14 publications

References 12 publications

Evaluation of pulse crops’ functional diversity supporting food production

Evaluation of pulse crops’ functional diversity supporting food production

Proteins patterns of eight genera of the Asteraceae family

Analysis of similarities within different date palm cultivars of Al-Ahsa oasis in Saudi Arabia

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