Seed Priming with Ascorbic Acid Improves Drought Resistance of Wheat

Abstract

The study, consisting of two independent experiments, was conducted to evaluate the role of seed priming with ascorbic acid (AsA) in drought resistance of wheat. In the first experiment, seeds of wheat cultivars Mairaj‐2008 and Lasani‐2008 were either soaked in aerated water (hydropriming) for 10 h or not soaked (control). In the second experiment, seeds of same wheat cultivars were soaked in aerated (2 mm) AsA solution (osmopriming) or water (hydropriming) for 10 h. In both experiments, seeds were sown in plastic pots (10 kg) maintained at 70 % and 35 % of water‐holding capacity designated as well watered and drought stressed, respectively. Both experiments were laid out in a completely randomized design with six replications. Drought caused delayed and erratic emergence and disturbed the plant water relations, chlorophyll contents and membranes because of oxidative damage; however, root length in cultivar Lasani‐2008 was increased under drought. Hydropriming significantly improved the seedling emergence and early growth under drought and well‐watered conditions; however, improvement was substantially higher from osmopriming with AsA. Similarly, osmopriming with AsA significantly improved the leaf emergence and elongation, leaf area, specific leaf area, chlorophyll contents, root length and seedling dry weight. Owing to increase in proline accumulation, phenolics and AsA, by seed priming with AsA, plant water status was improved with simultaneous decrease in oxidative damages. These improved the leaf emergence and elongation, and shoot and root growth under drought. However, there was no difference between the cultivars in this regard. In conclusion, osmopriming with AsA improved the drought resistance of wheat owing to proline accumulation and antioxidant action of AsA and phenolics, leading to tissue water maintenance, membrane stability, and better and uniform seedling stand and growth.

Consequences of Seed Priming with Salicylic Acid and Hydro Priming on Smooth Vetch Seedling Growth under Water Deficiency

“…As shown in Table 1, when soil moisture is sufficient (100% FC), the differences between primed and non-primed seeds was statistically negligible yet under severe water stress there was a substantial gap between them. However, Farooq et al (2013) expressed that the better germination start caused by seed priming leads to consequent greater seedling growth under both well watered and water deficit conditions. Seed priming with different compounds including water, urea and KNO 3 considerably improves germination and early growth of Chinese cabbage under water stress conditions (Yan, 2015).…”

Soluble amino acid profile, mineral nutrient and carbohydrate content of maize kernels harvested from plants submitted to ascorbic acid seed priming

“…Some studies have tested pre-sowing seed treatments (seed priming) using hormones (Carvalho et al 2011, Sneideris et al 2015 and vitamins such as ascorbic acid (AsA) to improve the stress tolerance (Farooq et al 2013, Alcântara et al 2015. AsA treatment increased the amount of soluble carbohydrates, proteins and soluble amino ASCORBIC ACID MAIzE SEED PRIMING 697 acids in beans (Azooz et al 2013), and increased the proline content in wheat (Farooq et al 2013), however these analyses were performed at the vegetative stage.…”

Alternative Approach for Drought Tolerance; Seed Priming and Physiology

“…Different types of priming treatments were recorded to enhance drought tolerance in many plants. Seed priming with ascorbic acid, potassium salts improved the drought resistance of wheat [16] [21].…”