Seed priming with antioxidants improves sunflower seed germination and seedling growth under unfavorable germination conditions

Draganic, I.; Lekić, Slavoljub

doi:10.3906/tar-1110-16

Cited by 14 publications

(12 citation statements)

References 27 publications

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“…Seed pre-hydration treatments using synthetic and natural compounds have been reported to be quite effective in alleviating and repairing stress-induced cellular impairments in several agriculturally important species, including the species of this study, Brassica oleracea (Abdolahi et al, 2012;Jisha et al, 2013) and Lactuca sativa (Varier et al, 2010;Carrozzi et al, 2012). Seeds treatments involving soaking in aqueous antioxidant solutions such as ascorbic acid (Burguieres et al, 2007;Yan et al, 2015), glutathione (Draganić and Lekić, 2012;Xia et al, 2020), trolox as tocopherol (Afzal et al, 2006;Draganić and Lekić, 2012) and gallic acid (Singh et al, 2017;Zeid et al, 2019) have been reported to neutralise the harmful oxidants capable of causing seed deterioration, and enhance seed germination, vigour and subsequent seedling growth in several species. Likewise, glycerol, a known radioprotectant (Chakrabarti et al, 1996;Yatim et al, 2016), has been suggested to scavenge and/or reduce the formation of harmful oxidants in seed embryonic axes exposed to desiccation and ultra-low temperature stress (Sershen et al, 2012).…”

Section: Rationale and Motivation For This Studymentioning

confidence: 88%

“…A rising proportion of AS production in seed lots that are still viable and capable of producing a high germination capacity is a symptom of deterioration (Matthews, 1985). A previous study on the effect of exogenous application of antioxidants prior to the ageing of sunflower seed showed an increased number of AS (Draganić and Lekić, 2012). A gradual loss of seed vigour resulting in the eventual loss of viability has been reported…”

Section: Effects Of Exogenous Application Of Antioxidants On Vigour V...mentioning

confidence: 99%

“…Havas (1935) reported accelerated seedling growth and improved shoot and root length of wheat as a result of AA application. Draganić and Lekić (2012) reported that application of exogenous antioxidants such as AA, glutathione, trolox and their combination favoured shoot and root growth of normal sunflower seedlings, while antioxidants, especially AA, have also been reported to stimulate shoot development in in vitro systems (Gupta and Datta, 2004). Ascorbic acid-linked improvement of growth was correlated with meristematic activity in shoots of Pisum sativum (Noctor, 2006).…”

Section: Effects Of Exogenous Application Of Antioxidants On Vigour V...mentioning

confidence: 99%

“…Classical seed priming protocols originally developed decades ago (Khan et al, 1980;Rowse, 1992;Taylor et al, 1992;Taylor et al, 1998), involve but are not limited to bio priming (Callan et al, 1991;Carrozzi et al, 2012;Mahmood et al, 2016), hydro priming (Welbaum et al, 1998b;Waqas et al, 2019) and the use of non-permeating organic osmotica, termed osmoconditioning (Khan et al, 1980;Liu et al, 1996). These protocols have been shown to enhance germination rate (Bradford et al, 1990), germination/emergence capacity (Khan, 1992;Jett et al, 1996), seedling vigour and tolerance to stresses (Delian et al, 2017), speed of emergence, vigour, biomass accumulation and leaf photosynthetic efficiency (Draganić and Lekić, 2012;Malik and Ashraf, 2012;Shah et al, 2019).…”

Section: Rationale and Motivation For This Studymentioning

confidence: 99%

“…Among the compounds of major importance in the antioxidant pathway of plants, glutathione plays a significant role in the cellular redox signalling networks influencing growth, development and defence (Foyer and Noctor, 2005;Jisha et al, 2013). Glutathione and tocopherol used as seed pre-hydration treatments resulted in increased seedling length of Helianthus annuus (Draganić and Lekić, 2012). In addition, pre-hydration treatment of seeds with other antioxidant solutions has been reported to improve seed performance in several species.…”

Section: Redoxprimingmentioning

confidence: 99%

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Physiological and biochemical investigations into the reinvigoration of deteriorated Brassica oleracea L. (Cabbage) and Lactuca sativa L. (Lettuce) seeds with antioxidants and inorganic salt solutions.

Adetunji

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This study focused on reinvigorating deteriorated Brassica oleracea L. (cabbage) and Lactuca sativa L. (lettuce) seeds with antioxidants and inorganic (electrolysed and non-electrolysed) salt solutions. All pre-treatment solutions were applied to fresh (control) and controlled deteriorated (CDd) seeds at 75% viability (P75), 50% viability (P50) and 25% viability (P25). The pre-hydration treatments were compared in terms of their effects on seed germination, seedling vigour, electrolyte conductivity (EC), accumulation of lipid peroxidation products, protein carbonylation (PC), antioxidant enzymes and germination enzymes. The study also investigated the effects of invigorating CDd seeds with selected antioxidants on subsequent seedling growth, gas exchange and photochemistry under greenhouse conditions. Controlled deterioration, in general, resulted in the loss of seed vigour and viability but at higher rates in lettuce than cabbage, and increased EC and PC, and lowered antioxidant and germination enzymes activities in both species. However, significant lipid peroxidation was only recorded in lettuce seeds. Antioxidant pre-treatments enhanced viability of CDd seeds of cabbage at P25 and lettuce at P50 and P25, and improved seedling vigour of fresh, P75 and P50 cabbage, and P50 and P25 lettuce seeds. Antioxidant pretreatments reduced EC and lipid peroxidation in lettuce seeds, while PC was reduced in both species. The treatments also elevated antioxidant and germination enzymes activities in P25 cabbage seeds and P50 and P25 lettuce seeds. The inorganic salt pre-treatments did not enhance percentage seedling production in CDd cabbage seeds. However, in CDd lettuce seeds, Cacontaining solutions and electolysed (cathodic water) treatments promoted percentage normal seedling production and enhanced seedling vigour irrespective of pre-treatment solution pH or seed deterioration level. In the greenhouse studies, certain antioxidants promoted seedling vigour and leaf area in both species and enhanced shoot dry weight and gas exchange in lettuce. In summary, CDd lettuce seeds responded better to both types of invigoration (i.e., antioxidants and inorganic salt solutions) than cabbage seeds, while antioxidants appeared to be relatively more beneficial than inorganic salts in both species. The results suggest that the benefits of certain pre-treatment solutions were based on the enhancement of the activities of key antioxidant and germination enzymes, and the efficiency of photosynthesis at the early stages of growth. The results argue for the use of these seed pre-hydration treatments for mitigating poor stand establishment brought about by seed ageing, and as a useful approach to reinvigorating seeds in long-term storage collections for ensuring global food security.

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Section: Rationale and Motivation For This Studymentioning

confidence: 88%

Section: Effects Of Exogenous Application Of Antioxidants On Vigour V...mentioning

confidence: 99%

Section: Effects Of Exogenous Application Of Antioxidants On Vigour V...mentioning

confidence: 99%

Section: Rationale and Motivation For This Studymentioning

confidence: 99%

Section: Redoxprimingmentioning

confidence: 99%

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Physiological and biochemical investigations into the reinvigoration of deteriorated Brassica oleracea L. (Cabbage) and Lactuca sativa L. (Lettuce) seeds with antioxidants and inorganic salt solutions.

Adetunji

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Testing seed germination from herbaria: Application of seed quality enhancement techniques and implication for plant resurrection and conservation

Albani Rocchetti,

Brancaleoni,

Caneva

et al. 2024

TAXON

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Herbaria are an important source of data and material useful in many fields, including plant conservation. Seeds preserved in herbarium specimens may have the potential to germinate, although few studies focused on this topic. Here, the first systematic assessment of six techniques, including priming techniques and melatonin application, aimed at improving the germination of seeds from herbarium specimens is presented. Seed germination of 26 species common in Europe, some of which congeneric to extinct species, collected in herbaria and in the wild (20,549 seeds in total, including 19,509 from 297 herbarium specimens from 8 different herbaria) was tested with the following treatments: exogenous melatonin addition to the germination medium, priming with melatonin, osmopriming, hydropriming for 24 and 48 hours, standard soil, heat sterilization and gibberellins addition. More than 85% of the fresh seeds and 1% of the seeds collected in herbaria germinated, including seeds older than 50 years. Data show that treatment with exogenous melatonin had a positive effect on the germination of fresh seeds, but a negative effect on the germination of herbarium‐derived seeds. Furthermore, osmopriming treatment had a slightly positive effect on the germination of herbarium‐derived seeds. Osmopriming and exogenous melatonin addition seem to be promising techniques that need further investigation and improvement and might be useful for the development of an optimal germination protocol for old and herbarium‐derived seeds. The germination of seeds from herbaria could be an important tool in plant conservation, with the aim of reversing the extinction trend of many species through de‐extinction, safeguarding biodiversity, and genetic variability. This study provides preliminary data for the development of germination protocols, especially for old seeds of species of conservation interest, to maximise the chance of recovering lost genetic diversity and leading to the first de‐extinction ever.

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Seed osmopriming with polyethylene glycol (PEG) enhances seed germination and seedling physiological traits of Coronilla varia L. under water stress

Ma,

Wei,

Han

et al. 2024

PLoS ONE

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Water stress can adversely affect seed germination and plant growth. Seed osmopriming is a pre-sowing treatment in which seeds are soaked in osmotic solutions to undergo the first stage of germination prior to radicle protrusion. Seed osmopriming enhances germination performance under stressful environmental conditions, making it an effective method to improve plant resistance and yield. This study analyzed the effect of seed osmopriming with polyethylene glycol (PEG) on seed germination and physiological parameters of Coronilla varia L. Priming treatments using 10% to 30% PEG enhanced germination percentage, germination vigor, germination index, vitality index, and seedling mass and reduced the time to reach 50% germination (T50). The PEG concentration that led to better results was 10%. The content of soluble proteins (SP), proline (Pro), soluble sugars (SS), and malondialdehyde (MDA) in Coronilla varia L. seedlings increased with the severity of water stress. In addition, under water stress, electrolyte leakage rose, and peroxidase (POD) and superoxide dismutase (SOD) activities intensified, while catalase (CAT) activity increased at mild-to-moderate water stress but declined with more severe deficiency. The 10% PEG priming significantly improved germination percentage, germination vigor, germination index, vitality index, and time to 50% germination (T50) under water stress. Across the water stress gradient here tested (8 to 12% PEG), seed priming enhanced SP content, Pro content, and SOD activity in Coronilla varia L. seedlings compared to the unprimed treatments. Under 10% PEG-induced water stress, primed seedlings displayed a significantly lower MDA content and electrolyte leakage than their unprimed counterparts and exhibited significantly higher CAT and POD activities. However, under 12% PEG-induced water stress, differences in electrolyte leakage, CAT activity, and POD activity between primed and unprimed treatments were not significant. These findings suggest that PEG priming enhances the osmotic regulation and antioxidant capacity of Coronilla varia seedlings, facilitating seed germination and seedling growth and alleviating drought stress damage, albeit with reduced efficacy under severe water deficiency.

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Seed priming with antioxidants improves sunflower seed germination and seedling growth under unfavorable germination conditions

Cited by 14 publications

References 27 publications

Physiological and biochemical investigations into the reinvigoration of deteriorated Brassica oleracea L. (Cabbage) and Lactuca sativa L. (Lettuce) seeds with antioxidants and inorganic salt solutions.

Physiological and biochemical investigations into the reinvigoration of deteriorated Brassica oleracea L. (Cabbage) and Lactuca sativa L. (Lettuce) seeds with antioxidants and inorganic salt solutions.

Testing seed germination from herbaria: Application of seed quality enhancement techniques and implication for plant resurrection and conservation

Seed osmopriming with polyethylene glycol (PEG) enhances seed germination and seedling physiological traits of Coronilla varia L. under water stress

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