Seed Pre-Conditioning With Ambiol®, a Derivative of 5-Hydroxybenzimidazole, Acclimates Tomato (Lycopersicon Esculentum Mill.) Seedlings to Drought

MacDonald, Mason T.; Rajasekaran, L. R.; Hoyle, Jeff; Robinson, A. R.

doi:10.17660/actahortic.2008.774.49

Cited by 4 publications

(8 citation statements)

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“…A 10 mgÁL -1 Ambiol Ò pretreatment resulted in a 113% increase in DM, a 35% increase in leaf area, and a 7% increase in MII compared with untreated seedlings. These results are consistent with previous results that found 10 mgÁL -1 Ambiol Ò an effective treatment in tomatoes both with and without ABA deficiency (MacDonald and Rajasekaran, 2006;MacDonald et al, 2008).…”

Section: Discussionsupporting

confidence: 93%

“…Any of these benefits could be of real use to producers. However, most antioxidant preconditioning studies have been limited to observing responses in seedlings (MacDonald et al, 2008;Blake, 1999, 2002). It would be interesting to see how long the effects persist in mature plants.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Rajasekaran et al (2005) have also found that Bioprotect Ò 2 (a conifer-derived phenolic compound; discovered by Dr. Lada, NSAC, Truro, Nova Scotia, Canada) and glycinebetaine (a quaternary ammonium compound) effectively promote drought tolerance in carrots. Another compound of particular interest as a SPA is a synthetic antioxidant called Ambiol Ò , derived from 5-hydroxybenzimidazole, which stimulates growth in several species and acts as an antistress (MacDonald et al, 2008;Blake, 1999, 2002;Rajasekaran et al, 2005), antioxidant (Santrucek and Krepelka, 1988;Voronina et al, 2001), and antitranspirant compound (Darlington et al, 1996). Based on the work of Rajasekaran and Blake (2002), it has been shown that preconditioning carrot seeds with 10 mgÁL -1 Ambiol Ò can promote dry matter production under drought conditions by as much as 214% when compared with a droughted control.…”

mentioning

confidence: 99%

“…Based on the work of Rajasekaran and Blake (2002), it has been shown that preconditioning carrot seeds with 10 mgÁL -1 Ambiol Ò can promote dry matter production under drought conditions by as much as 214% when compared with a droughted control. Also, Ambiol Ò is effective at promoting growth in tomato seedlings under moisture deficit through increased photosynthesis, leaf area expansion, and water use efficiency (MacDonald, 2006;MacDonald et al, 2008). It has been proposed that Ambiol Ò functions by offering membrane protection to maintain photosynthesis as well as promoting root growth while decreasing transpiration to increase plant water potential.…”

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confidence: 99%

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Seed Preconditioning with Natural and Synthetic Antioxidants Induces Drought Tolerance in Tomato Seedlings

MacDonald¹,

Lada²,

Robinson³

et al. 2009

horts

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There is strong evidence that Ambiol® (a derivative of 5-hydroxybenzimazole) promotes drought tolerance in many plants; it is often suggested that this is the result of its antioxidant properties. Recent evidence has also shown that several natural antioxidants promote carrot germination under drought stress. Thus, it was hypothesized that seed preconditioning using natural antioxidants might confer drought tolerance. Ambiol®, ascorbic acid, β-carotene, lutein, and lycopene were chosen as antioxidants at concentrations of 0.1 mg·L−1, 1.0 mg·L−1, and 10 mg·L−1. A preconditioning treatment was applied by soaking tomato (Solanum lycopersicum L.) seeds in an antioxidant solution for 24 h. Of the antioxidants tested, 10 mg·L−1 Ambiol®, 1.0 mg·L−1 β-carotene, 1.0 mg·L−1 ascorbic acid, and 0.1 mg·L−1 lycopene were shown to increase shoot dry mass by 114%, 94%, 56%, and 83%, respectively, in droughted seedlings when compared with a droughted control. Similar benefits were observed in root dry mass, leaf area, photosynthesis, and water use efficiency. Proteins were extracted from the seeds of certain treatments, before and after germination, and separated using isoelectric focusing. Specific proteins were found to be induced through all preconditioning treatments, whereas Ambiol® and β-carotene were found to induce specific proteins, independent of those induced through imbibition, both before and after germination. This result suggests that Ambiol® and β-carotene evoke specific proteins that may confer drought tolerance to the key physiological processes studied. In addition, protein profiles of ascorbic acid, β-carotene, and Ambiol® after germination had fewer visible bands than the controls, suggesting an accelerated mobilization or conversion of proteins within the seed.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Seed Preconditioning with Natural and Synthetic Antioxidants Induces Drought Tolerance in Tomato Seedlings

MacDonald¹,

Lada²,

Robinson³

et al. 2009

horts

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show abstract

“…If it is understood that ABA regulates stomatal aperture to protect plants from drought, is ABA necessary for Ambiol to induce drought tolerance? From the results of MacDonald et al (2008MacDonald et al ( , 2009, it remains unclear whether the mode of action of Ambiol in promoting drought tolerance requires the presence of ABA or is independent of ABA, but as a result of similarities in the effects of ABA and Ambiol, it was hypothesized that ABA is required for Ambiol action to enhance drought tolerance. If this is so, inhibiting ABA synthesis will negate the beneficial effects of Ambiol.…”

mentioning

confidence: 99%

Ambiol Preconditioning Can Induce Drought Tolerance in Abscisic Acid-deficient Tomato Seedlings

MacDonald¹,

Lada²,

Hoyle³

et al. 2009

horts

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Ambiol, a derivative of 5-hydroxybenzimidazole, has been well documented to function as a growth promoter, an antistress compound, and an antioxidant when applied as a seed preconditioning agent. However, evidence suggests that Ambiol decreases transpiration and promotes root growth similar to the phytohormone abscisic acid (ABA), leading to the development of the hypothesis that Ambiol promotes drought resistance through an ABA-dependent pathway. The effect of 0 mg·L−1 and 10 mg·L−1 was tested on wild-type tomato seedlings (Lycopersicon esculentum Mill. var. Scotia), ABA-deficient flacca tomato seedlings, and ABA-inhibited (with fluridone) tomato seedlings. In both fluridone-treated and flacca seedlings, Ambiol preconditioning resulted in significant increases in shoot growth, root growth, leaf area, and plant height consistent with gains experienced by wild-type tomatoes. In addition, flacca tomatoes experienced increases in photosynthesis and water use efficiency consistent with wild-type tomatoes. Ambiol was able to confer benefits to drought-stressed tomatoes in ABA-deficient and ABA-inhibited conditions, suggesting that Ambiol functions through an ABA-independent pathway.

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The Benefits of Ambiol® in Promoting Germination, Growth, and Drought Tolerance can be Passed on to Next-Generation Tomato Seedlings

MacDonald

Lada

Robinson

et al. 2010

J Plant Growth Regul

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Seed Pre-Conditioning With Ambiol®, a Derivative of 5-Hydroxybenzimidazole, Acclimates Tomato (Lycopersicon Esculentum Mill.) Seedlings to Drought

Cited by 4 publications

References 0 publications

Seed Preconditioning with Natural and Synthetic Antioxidants Induces Drought Tolerance in Tomato Seedlings

Seed Preconditioning with Natural and Synthetic Antioxidants Induces Drought Tolerance in Tomato Seedlings

Ambiol Preconditioning Can Induce Drought Tolerance in Abscisic Acid-deficient Tomato Seedlings

The Benefits of Ambiol® in Promoting Germination, Growth, and Drought Tolerance can be Passed on to Next-Generation Tomato Seedlings

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