Seed phosphorus and inositol phosphate phenotype of barley low phytic acid genotypes

Dorsch, John A.; Cook, Annetta; Young, Kim Eun; Anderson, Joseph M.; Bauman, Andrew T.; Volkmann, Carla J.; Murthy, Pushpalatha P. N.; Raboy, Victor

doi:10.1016/s0031-9422(02)00610-6

Cited by 179 publications

(176 citation statements)

References 38 publications

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“…Before treatment assignment, wethers were fed an adaptation diet (Table 1) for 14 days. Subsequently, one of two finishing-diet treatments (Table 1) was randomly assigned to each wether: Harrington (HARR) variety or mutant-M 955 (M955; Dorsch et al, 2003) barley-grain diets. Barley grains (whole berry), grown at the University of Idaho Agricultural Research and Extension Centre (Tetonia, ID), were incorporated into the diet as a total mixed ration.…”

Section: Experiments 1 221 Animals and Treatment Assignmentmentioning

confidence: 99%

“…Regardless, digestibility of these minerals was consistently greater in wethers when the low-phytate mutant-M 955 compared to the normalphytate Harrington variety barley grain was included in the diet. In Experiment 1, rumen Table 7 Mineral concentration (mM) of rumen fluid supernatant (27,000 × g, 10 min) from mature wethers consuming diets formulated with either Harrington (HARR) variety or mutant-M 955 (M955; Dorsch et al, 2003) (Table 7). Post-experiment analysis of Experiments 1 and 2 treatment diets, revealed that mature wethers (Table 5) and wether lambs (Table 6) fed the mutant-M 955 treatment diets consumed more (P<0.03) daily Mg, Fe, and Zn, and Mg, Fe, and Ca, respectively, than those fed the HARR treatment.…”

Section: Effect Of Low-phytate Barley On Mineral Digestion and Metabomentioning

confidence: 99%

“…These mutant grains contain similar amounts of total P as the non-mutant varieties, but have substantially less P existing as phytate. For example, the low-phytate mutant-M 955 barley grain has been reported to contain <0.2 mg phytate P/g, representing >90% reduction in grain phytate compared to the non-mutant Harrington variety, which contained >2.0 mg phytate P/g (Dorsch et al, 2003). Non-ruminant performance and P utilisation increased when low-phytate mutant grains were fed in place of non-mutant types (Veum et al, 2002;Jang et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Dietary low-phytate mutant-M 955 barley grain alters phytate degradation and mineral digestion in sheep fed high-grain diets

Leytem

Taylor

Raboy

et al. 2007

Animal Feed Science and Technology

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Greater production demands for ruminants require increased dietary inclusion of high-energy feeds. Grains and oil seeds are most commonly used to enhance diet energy density. However, use of such feeds proportionally increases the amount of dietary phytate phosphorus (P), which the ruminant may not be able to fully utilise. Our objectives for this study were to determine the extent of phytate degradation and mineral digestion in wethers fed high-grain diets consisting of either a non-mutant or low-phytate mutant barley grain. In two separate experiments, mature Columbia wethers (n = 7) fitted with rumen and duodenal cannulas and Columbia × Polypay wether lambs (n = 8) were individually fed one of two finishing diets formulated with either non-mutant Harrington (HARR) variety or lowphytate mutant-M 955 (M955) barley grains. Total-P intake was similar (P=0.46-0.70) between the M955 and HARR treatments for mature (5756 and 5550 mg/day, respectively) and lamb (5207 and 4894 mg/day, respectively) wethers. Dietary water-soluble P was 3.6 times greater in M955 Author's personal copy 14 A.B. Leytem et al. / Animal Feed Science and Technology 138 (2007) 13-28 HARR diets and phytate P was 11 times greater in HARR versus M955 treatment diets. Apparent total-P digestion was similar between M955 and HARR treatments (P=0.52-0.69). More monoester P was identified in the duodenal chyme of mature wethers fed HARR treatment diet, presumably due to incomplete hydrolysis of phytate P in the rumen. Feeding M955, compared to HARR, treatment diet resulted in greater (P<0.05) apparent partial-tract digestion of calcium (Ca) and total-tract digestion of iron (Fe), magnesium (Mg), and zinc in mature wethers and apparent total-tract digestion of Mg and Fe and retention of Ca, Fe, and Mg in wether lambs. These results indicate that phytate in diets formulated with Harrington variety barley grain may not be fully digested in the rumen. Subsequent passage of partially digested phytate from the rumen may interfere with mineral digestion in wethers fed high-grain diets.

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Section: Experiments 1 221 Animals and Treatment Assignmentmentioning

confidence: 99%

Section: Effect Of Low-phytate Barley On Mineral Digestion and Metabomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dietary low-phytate mutant-M 955 barley grain alters phytate degradation and mineral digestion in sheep fed high-grain diets

Leytem

Taylor

Raboy

et al. 2007

Animal Feed Science and Technology

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“…Hegman et al (2001) reported that a mutation affecting MIPS expression could be the cause of the low phytate accumulation in the seeds, and mutants were shown to be defective in phytic acid biosynthesis. Defective phytic acid mutant lines from various plant species, including Hordeum vulgare (Hatzack et al 2000;Dorsch et al 2003), Zea mays , O. sativa (Larson et al 2000;Kuwano et al 2006) and G. max (Wilcox et al 2000), showed impaired seed development. Similarly, antisense transgenic lines of MIPS genes in Solanum tuberosum plants showed altered plant morphology (Keller et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Comparative Expression and Cellular Localization of Myo-inositol Phosphate Synthase (MIPS) in the Wild Type and in an EMS Mutant During Common Bean (Phaseolus vulgaris L.) Seed Development

et al. 2011

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In plants, the myo-inositol-1-phosphate synthase (EC 5.5.1.4; MIPS) is an evolutionarily conserved enzyme protein and catalyzes the synthesis of myo-inositol, which is a central molecule required for cell metabolism and plant growth. A full-length cDNA encoding common bean (Phaseolus vulgaris L.) MIPS (designated Pv_BAT93 MIPS) was isolated from seed of P. vulgaris (cv, BAT93) by rapid amplification of cDNA ends. The cDNA of Pv_BAT93 MIPS comprised 1,873 bp, which encodes 510 amino acids. The Pv_BAT93 MIPS gene was highly homologous with those of other plant species, such as P. vulgaris (cv, Taylor's Horticultural), Arabidopsis thaliana, Medicago sativa and Glycine max. DNA blot analysis indicated that at least three copies of MIPS are present in the common bean genome. RT-PCR analysis indicated that the Pv_BAT93 MIPS transcripts existed in developing seeds and other common bean tissues, including leaves, flowers, and cotyledons but showed lower levels expression in roots and stems. Real-time quantitative PCR showed that Pv_BAT93 MIPS gene is differentially expressed during common bean seed development. In situ hybridization of developing seeds in the wild type and in the ethyl methanesulfonate mutant showed that expression of Pv_BAT93 MIPS was identified in the embryo tissues, from the globular to late cotyledon stages, confirming a possible involvement of Pv_BAT93 MIPS in common bean seed development.

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“…Phytate typically represents about 75% of total seed phosphorus (Raboy, 1997). Most, if not all, of this phosphate storage product is dispensable since mutations that cause significant reduction in seed phytate do not affect seed development in barley (Hordeum vulgare; Larson et al, 1998;Rasmussen and Hatzack 1998;Dorsch et al, 2003), maize (Zea mays; Raboy and Gerbasi 1996;Raboy et al, 2000;Shi et al, 2003Shi et al, , 2005, soybean (Glycine max; Wilcox et al, 2000;Hitz et al, 2002), wheat (Triticum aestivum; Guttieri et al, 2004), rice (Oryza sativa; Larson et al, 2000), or Arabidopsis (Arabidopsis thaliana; Stevenson-Paulik et al, 2005). Almost 50% of the phosphorus fertilizer applied worldwide ends up as phytate in crop seeds (Lott et al, 2000).…”

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confidence: 99%

Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase from Maize: Molecular and Biochemical Characterization

Sun¹,

Thompson²,

Lin³

et al. 2007

Plant Physiol.

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Discovery R&D, Dow AgroSciences, Indianapolis, Indiana 46268 Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, an enzyme encoded by the gene IPK1, catalyzes the terminal step in the phytic acid biosynthetic pathway. We report here the isolation and characterization of IPK1 cDNA and genomic clones from maize (Zea mays). DNA Southern-blot analysis revealed that ZmIPK1 in the maize genome constitutes a small gene family with two members. Two nearly identical ZmIPK1 paralogs, designated as ZmIPK1A and ZmIPK1B, were identified. The transcripts of ZmIPK1A were detected in various maize tissues, including leaves, silks, immature ears, seeds at 12 d after pollination, midstage endosperm, and maturing embryos. However, the transcripts of ZmIPK1B were exclusively detected in roots. A variety of alternative splicing products of ZmIPK1A were discovered in maize leaves and seeds. These products are derived from alternative acceptor sites, alternative donor sites, and retained introns in the transcripts. Consequently, up to 50% of the ZmIPK1A transcripts in maize seeds and leaves have an interrupted open reading frame. In contrast, only one type of splicing product of ZmIPK1B was detected in roots. When expressed in Escherichia coli and subsequently purified, the ZmIPK1 enzyme catalyzes the conversion of myo-inositol 1,3,4,5,6-pentakisphosphate to phytic acid. In addition, it is also capable of catalyzing the phosphorylation of myo-inositol 1,4,6-trisphosphate, myo-inositol 1,4,5,6-tetrakisphosphate, and myo-inositol 3,4,5,6-tetrakisphosphate. Nuclear magnetic resonance spectroscopy analysis indicates that the phosphorylation product of myoinositol 1,4,6-trisphosphate is inositol 1,2,4,6-tetrakisphosphate. Kinetic studies showed that the K m for ZmIPK1 using myo-inositol 1,3,4,5,6-pentakisphosphate as a substrate is 119 mM with a V max at 625 nmol/min/mg. These data describing the tissue-specific accumulation and alternative splicing of the transcripts from two nearly identical ZmIPK1 paralogs suggest that maize has a highly sophisticated regulatory mechanism controlling phytic acid biosynthesis.

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Seed phosphorus and inositol phosphate phenotype of barley low phytic acid genotypes

Cited by 179 publications

References 38 publications

Dietary low-phytate mutant-M 955 barley grain alters phytate degradation and mineral digestion in sheep fed high-grain diets

Dietary low-phytate mutant-M 955 barley grain alters phytate degradation and mineral digestion in sheep fed high-grain diets

Comparative Expression and Cellular Localization of Myo-inositol Phosphate Synthase (MIPS) in the Wild Type and in an EMS Mutant During Common Bean (Phaseolus vulgaris L.) Seed Development

Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase from Maize: Molecular and Biochemical Characterization

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