Seed morphology using SEM techniques for identification of useful grasses in Dera Ghazi Khan, Pakistan

Ahmad, Shafiq; Zafar, Muhammad; Ahmad, Mushtaq; Ali, Muhammad Ishtiaq; Sultana, Shazia; Rashid, Naim; Butt, Maryam Akram; Shah, Syed Nasar; Özdemir, Fethi Ahmet; Kutlu, Mehmet; Afza, Rabia; Usma, Anwer; Nazish, Moona

doi:10.1002/jemt.23408

Cited by 13 publications

(11 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously, much work has been conducted on systematics to identify different species by seed characters using SEM ( Ahmad et al, 2020 ), but there has been little reports on the correlation between seed internal ultrastructure and persistence. It was observed that ABA produced by seed embryos contributed to germination arrest upon imbibition and then maintained viability ( Chahtane et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Rising Shallow Groundwater Level May Facilitate Seed Persistence in the Supratidal Wetlands of the Yellow River Delta

Peng²,

Cui³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

The saline groundwater level of many supratidal wetlands is rising, which is expected to continue into the future because of sea level rise by the changing climate. Plant persistence strategies are increasingly important in the face of changing climate. However, the response of seed persistence to increasing groundwater level and salinity conditions is poorly understood despite its importance for the continuous regeneration of plant populations. Here, we determined the initial seed germinability and viability of seven species from supratidal wetlands in the Yellow River Delta and then stored the seeds for 90 days. The storage treatments consisted of two factors: groundwater level (to maintain moist and saturated conditions) and groundwater salinity (0, 10, 20, and 30 g/L). After retrieval from experimental storage, seed persistence was assessed. We verified that the annuals showed greater seed persistence than the perennials in the supratidal wetlands. Overall, seed persistence was greater after storage in saturated conditions than moist conditions. Salinity positively affected seed persistence under moist conditions. Surprisingly, we also found that higher groundwater salinity was associated with faster germination speed after storage. These results indicate that, once dispersed into habitats with high groundwater levels and high groundwater salinity in supratidal wetlands, many species of seeds may not germinate but maintain viability for some amount of time to respond to climate change.

show abstract

Section: Discussionmentioning

confidence: 99%

Rising Shallow Groundwater Level May Facilitate Seed Persistence in the Supratidal Wetlands of the Yellow River Delta

Peng²,

Cui³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…In Pakistan various studies have been conducted on microscopic characterization of plant species. The light and scanning microscopic characterization of various groups has been done on various groups of plants (Bano et al, 2020; Ahmad et al, 2013; Ahmad et al, 2020; Ahmad et al, 2020; Ashfaq et al, 2020a) while microscopic characterization of specific groups include Bryophytes (Khan et al, 2020); Gymnosperms (Khan et al, 2019); Pteridophytes (Shah et al, 2020; Shah et al, 2020; Shah et al, 2021); Some of the specific families including Asteraceae (Ahmad et al, 2013; Bano, Ahmad, Zafar, Sultana, & Khan, 2015; Ayaz et al, 2019); Mimosaceae (Khan et al, 2019; Alam et al, 2019; Khan et al, 2021); Convolvulaceae (Ashfaq et al, 2020b); Cactaceae (Majeed et al 2020); Capparidaceae (Mir et al, 2019); Amaranthaceae (Hussain et al, 2018; Nazish et al, 2020); Acanthaceae (Raza et al, 2020); Caryophyllaceae (Ullah et al, 2016; Ullah et al, 2019); Poaceae (Shaheen et al, 2018; Ullah et al, 2016; Khan et al, 2019; Usma et al, 2019; Ahmad 2020; Ullah et al, 2021); Lamiaceae (Gul et al, 2021; Bano et al, 2020); Papilionoideae (Rashid et al, 2019; Rashid et al, 2021; Waheed et al, 2021); Eragrostideae‐Poaceae (Ahmad et al, 2011); Solanaceae (Kayani et al 2019; Ashfaq et al, 2020a); Papilionaceae (Bano et al, 2019); Apiaceae (Kalsoom et al, 2019); Vitaceae (Lubna et al, 2019); Balsaminaceae (Rahman, Ahmad, Zafar, Mumtaz, & Shah, 2017); Caesalpiniaceae (Ullah et al, 2019); Euphorbiaceae (Zahra, Ahmad, Shinwari, Zafar, & Sultana, 2014). However, no work has been done on identification of A. flavus in various areas of Pakistan using microscopic techniques especially SEM.…”

Section: Introductionmentioning

confidence: 99%

Light and scanning electron microscopic characterization of aflatoxins producing Aspergillus flavus in the maize crop

Seerat

Akram

Qureshi

et al. 2022

Microscopy Res & Technique

View full text Add to dashboard Cite

Maize (Zea mays L.) is considered as one of the main cereals, used as a source of food, forage, and processed products. The loss of maize productivity is reported due to effect on roots, stalks, ears, and kernels mainly caused by many fungi. Among these fungal pathogens of maize, Aspergillus flavus (A. flavus) are the most prevalent that produces highly toxigenic aflatoxins that are highly carcinogenic to the consumers.The present study is confined to isolate and characterize the A. flavus from maize seeds for accurate identification that can be helpful for determination and management of aflatoxins in maize crop. Eighty stored seed samples of maize were collected from warehouses where seeds are stored for food and feeding purposes. For the isolation of A. flavus, Potato Dextrose Agar was used. Isolated fungi were identified macro and microscopically using light microscope and scanning electron microscope.A total of 212 Aspergillus isolates were identified based on macro-morphological and micro-morphological characteristics. The results showed that A. flavus colonies were granular, flat with yellow-green to deep yellow-green colony color having a white border and compact, spherical spore heads. Rapid rate of growth was observed maturing in about 3-5 days. In microscopic features, A. flavus have apically swollen conidiophores with various conidia bearing cells in long and dry chains. Spherical conidial heads were split into several columns ranging 300-400 μm in diameter. This will be helpful for farmers, researchers and traders in future for correct identification of sources of aflatoxins. Research Highlights• Maize seed samples were collected from Pothohar region of Pakistan.• The fungi were isolated on PDA.• Aspergillus flavus was identified macro-morphologically by observing growth rate, colony color and texture.• Furthermore, these fungi were identified micro-morphologically by using light and scanning electron microscope.• The 212 Aspergillus flavus strains were isolated and identified.

show abstract

“…Micromorphological character of seeds are studied through SEM which is less costlier, most reliable, easy, and time saving as compared to other modern techniques (Ayaz et al, 2020;Aziz et al, 2021;Rashid et al, 2021). The surface morphology of proposed green nanocatalyst has been determined using SEM technique which has been proved very effective in the field of plant taxonomy because it is used to explore the surface features of vegetative and reproductive organs (Ahmad et al, 2020;Usma et al, 2019). It also helps to illustrate the variations in seeds among the different member of the same family.…”

Section: Introductionmentioning

confidence: 99%

Scanning electron microscopy as a tool for authentication of biodiesel synthesis from Linum usitatissimum seed oil

Niazi

Ahmad

Elnaggar

et al. 2021

Microscopy Res & Technique

Self Cite

View full text Add to dashboard Cite

Utilization of renewable and alternative energy feedstocks such as nonedible seeds oil to deal with the increasing energy crises and related ecological concerns have gained the attention of researchers. Biodiesel is an efficient and renewable substitute for diesel engine. This work investigates the potential of inexpensive nonedible seed oil of Linum usitatissimum to synthesize biodiesel using iron sulfate green nanocatalyst through the process of transesterification. Flax seed contains about 37.5% oil content estimated through Soxhlet apparatus. Light microscopy revealed that seed size varies from 3.0 to 6.0 cm in length, 2.0 to 3.3 cm in width, and 0.7 to 1.0 mm in diameter. Color of seed varied from yellow to brown. Characterization of biodiesel is performed through GC–MS and FTIR. Scanning electron microscopy was carried out to study the morphological features of seed coat. Catalyst was characterized by scanning electron microscopy, energy diffraction X‐ray, and X‐ray diffraction. The diffraction peaks of Fe3O4 green nanoparticles were found to be in 2θ values, 30.24°, 35.62°, 38.26°, 49.56°, 57.12°, and 62.78°. Fuel properties of biodiesel are also determined and compared with ASTM standards. Linum usitatissimum biodiesel has density 0.8722 (15°C kg/L), kinetic viscosity 5.45 (40°C cSt), flash point (90°C), pour point (−13°C), cloud point (−9°C), sulfur (0.0432% wt), and total acid number (0.245 mg KOH/g). It is concluded that L. usitatissimum seed oil is a highly potential source for biodiesel production to cope with the challenge of present energy demand.

show abstract

Seed morphology using SEM techniques for identification of useful grasses in Dera Ghazi Khan, Pakistan

Cited by 13 publications

References 21 publications

Rising Shallow Groundwater Level May Facilitate Seed Persistence in the Supratidal Wetlands of the Yellow River Delta

Rising Shallow Groundwater Level May Facilitate Seed Persistence in the Supratidal Wetlands of the Yellow River Delta

Light and scanning electron microscopic characterization of aflatoxins producing Aspergillus flavus in the maize crop

Scanning electron microscopy as a tool for authentication of biodiesel synthesis from Linum usitatissimum seed oil

Contact Info

Product

Resources

About