Seed morphology of the subfamily Helleboroideae (Ranunculaceae) and its systematic implication

Ghimire, Balkrishna; Jeong, Mi Jin; Choi, Go Eun; Lee, Hayan; Suh, Gang Uk; Heo, Kweon; Ku, Ja Jung

doi:10.1016/j.flora.2015.07.004

Cited by 16 publications

(15 citation statements)

References 21 publications

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“…Seed coat ultra‐structural features using SEM play important roles in the classification and phenetic linkages of species and practical features to study systematic relationships in variety of families, whereas seed micromorphology has proven importance in certain cases (Koul, Nagpal, & Raina, ; Rashid et al, ; Yoshizaki, ). Seed coat and their structural features are successfully applicable in evolutionary relationships and in addition simplify the affiliation among different genera and species within the tribe (Ghimire et al, ). SEM discloses seed outer surface characters that are successfully applicable to solve phylogenetic relationships and the systematic problems (Karihaloo & Malik, ).…”

Section: Introductionmentioning

confidence: 99%

Micromorphological observation of seed coat of Eucalyptus species (Myrtaceae) using scanning electron microscopy technique

Luqman

Zafar

Ahmad

et al. 2018

Microscopy Res & Technique

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Seed shape, surface cells shape, arrangement, anticlinal wall pattern, and periclinal wall protuberances were recorded for nine species of Eucalyptus (Myrtaceae) using scanning electron microscopy to determine the importance of seed morphological characters as an additional tool for identification. Most of the seeds were found ovate and some seeds were elliptic and cuboid in shape. Almost reticulate regular seed surface patterns were observed. Four types of surface cells were examined; diamond, elliptic, oblong, and irregular. Majority of the seeds showed raised anticlinal wall level and diversity from wavy to puzzle in pattern. Periclinal wall may be glabrous or having protuberances that were rhombus and bullate in shape. Both macro-and micromorphological characters can provide basis for classification and delimitation of genus Eucalyptus. Research highlights• Seeds quantitative characters of nine Eucalyptus species as seed length, width, and weight.• Macromorphological characters of seeds including seed color, helium position, and seed shape.• Micromorphological characters include seed surface, periclinal wall, and anticlinal wall investigation under scanning electron microscope.• Ultra-seed sculpturing features as an additional tool in identification. K E Y W O R D S eucalyptus, micromorphology, Myrtaceae, seed, SEM

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Section: Introductionmentioning

confidence: 99%

Micromorphological observation of seed coat of Eucalyptus species (Myrtaceae) using scanning electron microscopy technique

Luqman

Zafar

Ahmad

et al. 2018

Microscopy Res & Technique

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“…Thus, macromorphological characters like shape and colour does not reflect the generic delimitation in tribe Nigelleae. Also Ghimire et al (2015). found these characters less significant for the generic taxonomy of Ranunculaceae subfam.…”

Section: Discussionmentioning

confidence: 91%

“…In addition, seed coat morphology, especially micromorphology, have provided useful taxonomic information for different genera of Ranunculaceae (Cappelletti and Poldini 1984, Karcz and Tomzcok 1987, Luo et al 2005, Dadandı et al 2009, Wang et al 2009, Antkowiak et al 2010, Emadzade et al 2010, Heiss et al 2011, Ghimire et al 2015, 2016. Half of the sections of Consolida (Constantinidis et al 2001) and tribe Delphinieae (Ghimire et al 2015) are well supported based on only seed characters. In contrast, seed morphology of Thalictrum L. shows low taxonomic value at the sectional level (Ghimire et al 2016).…”

Section: Numerical Analyses Of Seed Morphology and Its Taxonomic Signmentioning

confidence: 99%

“…Seed morphological characters have been considered significant traits for distinguishing among all taxonomic ranks in different plant families (Barthlott 1981, Arroyo-Cosultchi et al 2006, Fagundez and Izco 2011, Antkowiak et al 2018, Ghimire et al 2018). In addition, seed coat morphology, especially micromorphology, have provided useful taxonomic information for different genera of Ranunculaceae (Cappelletti and Poldini 1984, Karcz and Tomzcok 1987, Luo et al 2005, Dadandı et al 2009, Wang et al 2009, Antkowiak et al 2010, Emadzade et al 2010, Heiss et al 2011, Ghimire et al 2015, 2016. Half of the sections of Consolida (Constantinidis et al 2001) and tribe Delphinieae (Ghimire et al 2015) are well supported based on only seed characters.…”

Section: Numerical Analyses Of Seed Morphology and Its Taxonomic Signmentioning

confidence: 99%

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Numerical analyses of seed morphology and its taxonomic significance in the tribe Nigelleae (Ranunculaceae)

Aydın

Dönmez

2019

Nordic Journal of Botany

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We examined the seed morphology of Nigella, Garidella and Komaroffia to aid systematics and taxonomy. The seeds of 21 taxa in 20 species from Mediterranean and West Asian countries were studied using both zoom stereo and scanning electron microscopy (SEM). Principal coordinate analysis (PCoA) and unweighted average linkage analysis (UPGMA) were performed to assess the contribution of seed characters to the taxonomy of the tribe. Representative pictures from zoom stereo microscopy and SEM studies and measurement of seeds are presented. Most studied species showed unique seed surface sculpturing features, and seed shape, size and surface sculpturing separated the studied taxa into five main groups. However, the variation in seed shape and color were found to be in conflict with the generic taxonomy while macromorphological characters were found more congruent with the sectional taxonomy than with the generic taxonomy. Five main types of seed ornamentations – rugulose + striate, granulate, smooth, rugulose and mucronulate – were identified. Patterns in seed micromorphology strongly supported the sectional level taxonomy of Nigella. Two of the three sections of the genus were found to be well differentiated in terms of seed characters. Furthermore, seed morphology alone provide good support to Garidella as a distinct genus, but shows less taxonomic value for Komaroffia.

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“…A number of angiosperm taxa have already been intensively investigated in terms of seed morphology, in combination with phenetic or phylogenetic analyses at the genus level. In the past the variation in seed morphology has been used variously in plant systematics ranging from identification (Sako et al 2001, Khalik andVan der Maesen 2002) and taxonomic circumscription (Constantinidis et al 2001, Mendum et al 2001, Amini et al 2011, Sadeghian et al 2014, Ghimire et al 2015a, 2016a, Arabi et al 2017) to phylogenetic inference (Hufford 1997, Arambarri 2000 and character-state evolution (Fukuhara 1999, Simons andHedin 1999). Although quite a few embryological, floral, and carpological studies of Oleaceae have been carried out (Rohwer 1993, 1994, Sher and Weber 2009, Dadpour et al 2011, Naghiloo et al 2013, Ghimire and Heo 2014, Ghimire et al 2015b, studies on comparative seed morphology and seed coat anatomy are still scarce for the family.…”

Section: Introductionmentioning

confidence: 99%

Seed morphology and seed coat anatomy of Fraxinus, Ligustrum and Syringa (Oleeae: Oleaceae) and its systematic implications

Ghimire

Jeong

Suh

et al. 2018

Nordic Journal of Botany

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We have carried out a morphological and anatomical study of the seeds of 19 taxa representing two subtribes and three genera of the tribe Oleeae (Fraxinus, Ligustrum, and Syringa) and two species of Forsythia (Forsythieae) using scanning electron and light microscopy to evaluate the usefulness of seed characteristics for the identification of systematic relationships. Considerable differences were found in seed coat morphology both among and within genera. Three types of seed coat pattern – reticulate, colliculate, and tuberculate – were recognized. The shape of the testa epidermal cells was either elongated rectangular or polygonal with smooth, striated, or infolded secondary ornamentations. The seed coat was thickest in Ligustrum spp. and thinnest in Syringa spp. The exotesta of Fraxinus species was discontinuous due to the presence of longitudinal grooves on its surface. Based on 22 seed morphological characters, cladistic analysis resolved three well‐supported clades that matched the three genera well, with Forsythia species considered as an outgroup. Ligustrum foliosum, with reticulate ornamentation, concave periclinal walls, and raised anticlinal walls, was the most distinct species within the genus, whereas the flat periclinal and sunken anticlinal walls in Syringa fauriei were unique in Syringa. Similarly, infolded secondary ornamentation was only observed in Fraxinus rhynchophylla var. densata. Although the investigated specimens represent a limited sample, the results provide valuable support for the characterization of several taxa, particularly at the species level. Moreover, surface ornamentation, epidermal cell shape, periclinal and anticlinal wall structure, and the nature and structure of the exotesta are shown to be potentially useful for cladistic analysis and for identification of species in this alliance.

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Seed morphology of the subfamily Helleboroideae (Ranunculaceae) and its systematic implication

Cited by 16 publications

References 21 publications

Micromorphological observation of seed coat of Eucalyptus species (Myrtaceae) using scanning electron microscopy technique

Micromorphological observation of seed coat of Eucalyptus species (Myrtaceae) using scanning electron microscopy technique

Numerical analyses of seed morphology and its taxonomic significance in the tribe Nigelleae (Ranunculaceae)

Seed morphology and seed coat anatomy of Fraxinus, Ligustrum and Syringa (Oleeae: Oleaceae) and its systematic implications

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