Seed longevity phenotyping: recommendations on research methodology

Hay, Fiona R.; Valdez, R. B.; Lee, Jae Sung; Cruz, Pompe C. Sta.

doi:10.1093/jxb/ery358

Cited by 49 publications

(98 citation statements)

References 55 publications

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“…; Merck Co. Germany) and quantified based on retention time and amounts of standards (Darmstadt, Germany; Helios, Singapore). Seed longevity parameters were determined at IRRI through standard storage experiments (Hay et al, 2015(Hay et al, , 2018. After moisture content (MC) equilibration at 20°C, 60% RH, seeds were heat-sealed inside aluminium foil bags and placed at 45°C.…”

Section: Methodsmentioning

confidence: 99%

A high proportion of beta-tocopherol in vitamin E is associated with poor seed longevity in rice produced under temperate conditions

Lee

Kwak

Cho

et al. 2019

Plant Genet. Resour.

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Seeds of 15 diverse rice accessions, representing aus, indica, temperate japonica and tropical japonica subpopulations, were produced under temperate climate conditions in Korea and used for vitamin E analysis and seed storage experiments at 45°C and 10.9% seed moisture content. High γ-tocotrienol was significantly positively correlated with seed longevity. In addition, a high β-tocopherol proportion relative to δ-tocopherol was significantly negatively correlated with seed longevity. Using high-density single-nucleotide polymorphism marker data, DNA haplotype analysis showed clear allelic variations in the region of two S-adenosylmethionine synthetase genes: LOC_Os04g42095 and LOC_Os11g15410, which regulate the conversion of δ-tocopherol into β-tocopherol. Four indica accessions with rare and subpopulation-specific alleles showed a 2.3-fold lower β-/δ-tocopherol ratio compared with accessions from other subpopulations.

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Section: Methodsmentioning

confidence: 99%

A high proportion of beta-tocopherol in vitamin E is associated with poor seed longevity in rice produced under temperate conditions

Lee

Kwak

Cho

et al. 2019

Plant Genet. Resour.

Self Cite

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“…Generally, seed longevity is defined as the maximum time period that seeds maintain germination viability under ideal environmental conditions (Sano et al, ), and it is usually measured as the germination ratio after the long‐term storage (Liu et al, ; Rajjou et al, ; Wu et al, ). Seed longevity varies among different species and could be influenced by several environmental factors during seed formation, harvest and storage (Hay, Valdez, Lee, Sta, & C., ; Liu et al, ). Phases like embryogenesis, reserve accumulation and maturity drying are critically associated with the establishment of seed longevity (Rajjouand Debeaujon, ; Wu et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Genetic factors could greatly affect seed longevity during seed development (Bettey, Finch‐Savage, King, & Lynn, ; Finch‐Savage, Clay, Lynn, & Morris, ). When seeds are stored, seed longevity could be significantly influenced by several environmental factors, especially humidity and temperature (Hay et al, ; Nguyen, Keizer, Eeuwijk, Smeekens, & Bentsink, ; Wu et al, ). Studies of seed longevity under conventional storage conditions would take years to complete.…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide association study reveals loci associated with seed longevity in common wheat (Triticum aestivum L.)

Zuo

Chen

et al. 2019

Plant Breeding

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Seed longevity could significantly determine seed regeneration cycle and greatly affect wheat production. With the 90 K chip assays, a genome‐wide association study was performed to identify seed longevity‐related markers and loci in common wheat. Seed germination ratios (GR) under artificially ageing of 166 wheat accessions across three environments were evaluated to assess seed longevity. Totally, 23 longevity‐related loci were identified in the study, explaining 6.7%–11.4% of the phenotypic variations. Of these, QlgGR.cas‐1A and QlgGR.cas‐2B.2 were deemed as stable loci associated with wheat seed longevity. Fifteen loci were found overlapped with known quantitative trait loci or genes. Besides, QlgGR.cas‐1A, QlgGR.cas‐2B.2, QlgGR.cas‐3D.1, QlgGR.cas‐3D.2, QlgGR.cas‐4A.2, QlgGR.cas‐5A.1, QlgGR.cas‐5A.2 and QlgGR.cas‐6A.1 were colocated with seed dormancy‐related loci. Significant additive effects were obtained for seed longevity by pyramiding favourable alleles. Several candidate genes were found involved in signal transduction and stress resistance pathways by sequencing analysis of significantly longevity‐related molecular markers. These results might provide new sights into the genetic architecture of seed longevity.

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“…However, the development of novel, equally reliable methods is always desirable. This is particularly relevant when recent reports question the suitability of classical ageing methods as predictive tools of seed longevity [79].…”

Section: Discussionmentioning

confidence: 99%

Dynamic hydrolase labelling as a marker for seed quality in Arabidopsis seeds

Torre

Kaschani

Kaiser

et al. 2019

Biochemical Journal

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Seed quality is affected by different constituents of the seed. In general, seed lots are considered to be of high quality when they exhibit fast and homogeneous germination. When seeds are stored, they undergo different degrees of damage that have detrimental effects on their quality. Therefore, accurate prediction of the seed quality and viability levels of a seed lot is of high importance in the seed-producing industry. Here, we describe the use of activity-based protein profiling of proteases to evaluate the quality of artificially and naturally aged seeds of Arabidopsis thaliana. Using this approach, we have identified two protease activities with opposite behaviours in aged seeds of Arabidopsis that correlate with the quality status of the seeds. We show that vacuolar processing enzymes (VPEs) become more active during the ageing process, in both artificial and natural ageing treatments. Secondly, we demonstrate that serine hydrolases are active at the beginning of our artificial ageing treatment, but their labelling decreases along with seed viability. We present a list of candidate hydrolases active during seed germination and propose that these protease activities can be used in combination with VPEs to develop novel markers of seed quality.

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Seed longevity phenotyping: recommendations on research methodology

Cited by 49 publications

References 55 publications

A high proportion of beta-tocopherol in vitamin E is associated with poor seed longevity in rice produced under temperate conditions

A high proportion of beta-tocopherol in vitamin E is associated with poor seed longevity in rice produced under temperate conditions

Genome‐wide association study reveals loci associated with seed longevity in common wheat (Triticum aestivum L.)

Dynamic hydrolase labelling as a marker for seed quality in Arabidopsis seeds

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