Seed lipases: sources, applications and properties - a review

Barros, Márcio de; Fleuri, Luciana Francisco; Macêdo, Gabriela Alves

doi:10.1590/s0104-66322010000100002

Cited by 174 publications

(112 citation statements)

References 69 publications

Supporting

Mentioning

100

Contrasting

Unclassified

Order By: Relevance

“…After lipid hydrolysis, the energy necessary for radicle emergence is divided between the storage organs, such as cotyledons and the tube axis reserves, and for early seedling growth and development (Davies and Slack, 1981). In addition, lipase activity, enzymes responsible for the hydrolysis of triglycerides, increases rapidly in the post-germination stage, and is related to the mobilization of lipids (Abigor et al, 2002;Polizelli et al, 2008;Barros et al, 2010. ) The lipids are accumulated in seeds as triglycerides and degraded into glycerol and fatty acids, which are used in the synthesis of sucrose to provide energy and carbon skeletons for seedling growth (Borges and Rena, 1993;Buckeridge et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

Alterations in seed reserves of Dalbergia nigra ((Vell.) Fr All. ex Benth.) during hydration

et al. 2013

View full text Add to dashboard Cite

Seed imbibitions is the first stage of the germination process and is characterized by the hydration of tissues and cells and the activation and/or induction of the enzymes responsible for mobilizing reserves for respiration and the construction of new cell structures. The objective of this study was to investigate the alterations in reserve substances during slow hydration of Bahia Rosewood (Dalbergia nigra) seeds in water. Seeds from two different lots (Lot I and II) were placed in saturated desiccators (95-99% RH) to hydrate at 15 and 25 °C until water contents of 10, 15, 20 and 25% were reached. At each level of hydration, changes in lipid reserves, soluble carbohydrates, starch and soluble proteins were evaluated. The mobilization of reserves was similarly assessed in both lots, with no differences being observed between the two hydration temperatures. Lipid contents showed little variation during hydration, while the contents of soluble carbohydrates and starch decreased after the 15% water content level. Soluble proteins showed a gradual tendency to decrease between the control (dry seeds) up to 25% water content.

show abstract

Section: Resultsmentioning

confidence: 99%

Alterations in seed reserves of Dalbergia nigra ((Vell.) Fr All. ex Benth.) during hydration

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Lipases (EC 3.1.1.3) are serine hydrolases produced by animals (Goswami et al, 2012), plants (Barros et al, 2010) and microorganisms (Hassan et al, 2006); Carneiro et al, 2014), although the majority of lipases used for many biotechnological purposes are extracted from bacteria, fungi and yeast cultures. Microbial enzymes are often more useful than enzymes derived from other sources because of the higher stability and more convenient and safer production (Hasan et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Characterization and spray drying of lipase produced by the endophytic fungus Cercospora kikuchii

Costa-Silva

Souza

Oliveira

et al. 2014

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

-A lipase from the endophytic fungus Cercospora kikuchii was purified, biochemically characterized and the effects of spray drying on stabilization of the purified enzyme were studied. The lipase was purified 9.31-fold with recovery of 26.6% and specific activity of 223.6 U/mg. The optimum pH and temperature were 4.6 and 35 °C, respectively, while the Vmax was 10.28 µmol/min.mg -1 protein and Km 0.0324 mM. All the metal ions tested enhanced the enzyme activity. The lipase retained almost 100% activity in the presence of strong oxidants and was also resistant to Triton X, Tween 80 and 20 and SDS, as well as to proteases. The purified lipase was spray dried and kept until 85.2% of enzymatic activity. At least 70% of the enzymatic activity was maintained for spray dried purified lipase during the storage period. The lipase produced by Cercospora kikuchii has properties useful for industrial application and showed adequate stabilization and retention of its enzymatic activity after spray drying.

show abstract

“…[11][12][13][14][15] Essas enzimas encontram-se largamente distribuídas na natureza em tecidos animais e vegetais e biomassa microbiana. [11][12][13][14][15][16] Dentre elas, as lipases microbianas são as mais utilizadas industrialmente. 6,12,13 Por outro lado, as lipases vegetais apresentam algumas vantagens em relação às lipases microbianas e animais como ampla disponibilidade, baixo custo e elevada especificidade.…”

Section: Introductionunclassified

“…14-19 Os materiais de reserva presentes nas sementes como amido, proteínas e/ou lipídeos são hidrolisados por ação de hidrolases produzidas durante o processo de germinação tais como proteases, amilases e lipases para fornecer energia e componentes para o crescimento embrionário nos primeiros estágios de desenvolvimento. 16,17,24 Óleos e gorduras são convertidos em ácidos graxos livres que posteriormente são convertidos em açúcares por reações de β-oxidação. 24,25 Entretanto, a atividade lipásica também pode ser detectada em sementes dormentes de algumas oleaginosas como mamona e pinhão-manso.…”

Section: Introductionunclassified

Produção de concentrados de ácidos graxos por hidrólise de óleos vegetais mediada por lipase vegetal

Coelho¹,

Santos²,

Domingues³

et al. 2013

Quím. Nova

View full text Add to dashboard Cite

Recebido em 16/1/13; aceito em 8/5/13; publicado na web em 10/6/13 PRODUCTION OF CONCENTRATED FATTY ACIDS BY HYDROLYSIS OF VEGETABLE OILS CATALYZED BY PLANT LIPASE. The aim of this work was to verify the ability of enzymatic crude extract from dormant castor bean seeds to yield concentrated fatty acids by hydrolysis of polyunsaturated vegetable oils such as corn and sunflower. The enzymatic extract exhibited higher activity towards corn oil, which was selected for further studies to determine optimum hydrolysis conditions by factorial design. Maximum hydrolysis percentage (≈84%) was reached at 60% wt. oil:buffer acetate 100 mM pH 4.5, 33 °C and 5.0% wt. of crude extract after 70 min of reaction. These results suggest that the use of low-cost lipase from castor bean seeds has potential for oil hydrolysis.Keywords: oil hydrolysis; factorial design; plant lipase. [1][2][3] Estes números referem-se apenas à produção dos principais óleos e gorduras como soja, algodão, amendoim, girassol, colza, gergelim, milho, oliva, palma, coco, linhaça e mamona, e as gorduras de fonte animal como manteiga, sebo e peixe. 3 A modificação química destes óleos e gorduras mediada por catalisadores químicos é normalmente realizada em elevadas temperaturas e pressão (250 °C e 50 atm). Geralmente, estes processos fornecem produtos de composição química mista e/ou contaminada devido à ocorrência de reações indesejáveis como oxidação, desidratação e interesterificação que requerem etapas posteriores de purificação. Neste contexto, a modificação de óleos e gorduras catalisada por lipases vem se apresentando como uma alternativa atrativa para a indústria, principalmente quando são consideradas algumas das vantagens desta rota como maior rendimento do processo, menor consumo de energia, redução do teor de resíduos e introdução de rotas mais acessíveis de produção. [4][5][6][7][8][9][10] Lipases (glicerol éster hidrolases -E.C. 3.1.1.3) são enzimas que catalisam a hidrólise de ligações éster de óleos e gorduras com diferentes especificidades e podem, em meio orgânico, catalisar uma variedade de reações, como esterificação, interesterificação, transesterificação e aminólise. As lipases têm sido amplamente empregadas na produção de fármacos, emulsificantes, alimentos, perfumaria, diagnósticos médicos, compostos opticamente ativos, polímeros, aromas e fragrâncias, modificações de lipídeos para a produção de biodiesel e lipídeos estruturados e no pré-tratamento de efluentes com elevado teor de lipídeos gerados pelas indústrias de alimentos. [11][12][13][14][15] Essas enzimas encontram-se largamente distribuídas na natureza em tecidos animais e vegetais e biomassa microbiana. [11][12][13][14][15][16] Dentre elas, as lipases microbianas são as mais utilizadas industrialmente. 6,12,13 Por outro lado, as lipases vegetais apresentam algumas vantagens em relação às lipases microbianas e animais como ampla disponibilidade, baixo custo e elevada especificidade. 14-16 As principais fontes de lipases vegetais são o látex de frutos do gênero Carica como baba...

show abstract

Seed lipases: sources, applications and properties - a review

Cited by 174 publications

References 69 publications

Alterations in seed reserves of Dalbergia nigra ((Vell.) Fr All. ex Benth.) during hydration

Alterations in seed reserves of Dalbergia nigra ((Vell.) Fr All. ex Benth.) during hydration

Characterization and spray drying of lipase produced by the endophytic fungus Cercospora kikuchii

Produção de concentrados de ácidos graxos por hidrólise de óleos vegetais mediada por lipase vegetal

Contact Info

Product

Resources

About