Seed germination test as an alternative urine-based non-invasive pregnancy test in alpacas (<i>Vicugna pacos</i>)

Kubátová, Anna; Fedorova, Tamara

doi:10.5958/2277-8934.2016.00044.8

Cited by 2 publications

(3 citation statements)

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“…In the same time, standard deviation for seeds germination (Table 1) shows a small difference between test replicates of pregnant and non-pregnant females. Given that there is just one paper in sheep on this subject, it can be said that the results of wheat seed germination test in ewes are similar to those in 45 days pregnant cows (Rine et al 2014), buffaloes (Rahman and Saha, 2020) goats (Islam et al, 2014), pigs (Kumar et al, 2017) and alpacas (Kubátová and Fedorova, 2016).…”

Section: The Percent Of Germinationmentioning

confidence: 83%

“…Table 2. The urinalysis results obtained with dipstick pH kit for pregnant (1-10) and non-pregnant ewes (11)(12)(13)(14)(15)(16)(17)(18)(19)(20) after some seconds, depending by each parameter. Taking all of these into account, it can be speculated that, regarding herbivores, the dipstick measurements are suitable for pH evaluation with high applicability in field conditions, as Defontis et al (2013) had already showed in calves.…”

Section: Urinalysis Resultsmentioning

confidence: 99%

“…For sure these reasons, were certainly of supreme importance for such a highly organized Pharaonic Egypt society because wedding contracts with previously married women emphasize that interest (Ghalioungui et al, 1963) As soon as the results of this test were found to have benefits in humans, physicians started to test this method for animals, after Venna and Narendranath (1993) adopted suitable modifications to be used for pregnancy diagnosis in cattle. Nowadays, good results are obtained in pregnant cows ( Rahman and Saha, 2020), ewes (Islam et al, 2014), goats (Islam et al, 2014), sows (Kumar et al, 2017), and alpacas (Kubátová and Fedorova, 2016).Throughout the time, the interest for a precocious pregnancy diagnosis in animals has increased in terms of herd management. There is a study (Nirmala et al, 2008) that claims that urinary reproductive hormones (especially estrogen and progesterone) do not affect the seed germination or shoot length, but Lázničková et al (2020) believe that there is weak correlation between these hormones and the two parameters mentioned.…”

Section: Introductionmentioning

confidence: 99%

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Can Wheat Germination Test Be Used to Predict Pregnancy in Ewes?

et al. 2022

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Several invasive diagnosis methods to predict pregnancy in humans and animals were used in the past. Since Antiquity, grains germination test was used to predict more safely the pregnancy in women in order to avoid the possibility of miscarriage. The abscisic acid is a component of pregnant females’ urine that prevents germination of grains. Considering this principle, 20 samples of urine were collected from 10 pregnant ewes and 10 from non-pregnant animals. After urinalysis, 15 ml urine sample was added to 20 grains of wheat in a Petri dish, followed by a dilution of 1:4 with distilled water. Only water has been added in the control sample. There were performed duplicates for each animal. For 10 days, all plates were kept in a constant environmental temperature (22°C) and humidity (42%). Statistical analysis revealed that after 5 and 10 days, wheat grains belonging to pregnant ewes were less germinated, and values regarding shoot length were lower than those from the control group. Even if the number of samples is small, it can be concluded that wheat germination test can be used to predict pregnancy in ewes.

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Section: The Percent Of Germinationmentioning

confidence: 83%

Section: Urinalysis Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Can Wheat Germination Test Be Used to Predict Pregnancy in Ewes?

et al. 2022

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Evaluation of Sulfuric Acid, Barium Chloride, and Seed Germination Assay Methods as Early Pregnancy Detection Instruments in Cattle

Azmi¹,

Desem²,

Purba³

et al. 2020

J Kedokt Hewan

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This study aims to validate and re-evaluate the use of H2SO4, BaCl2, and seed germination assay (SGA) methods to detect early pregnancy in cattle. Validation was carried out in order to find out the actual test performance value, which include accuracy, sensitivity, and specificity.Validation test was done by reacting the urine of pregnant and non-pregnant cattle populations with H2SO4 solution diluted at a ratio of 1 : 4, with 1% BaCl2 solution or through germination of mung bean seeds in Petri dishes. The results of this study indicated that the H2SO4 and BaCl2 methods have poor accuracy (21.88% and 28.13%) with the sensitivity of 81.82% and 100% and specificity of 9.43% and 13.21%, respectively. Meanwhile, the SGA method had a fairly good accuracy (74.55%) with sensitivity and specificity of 45.46% and 81.82%, respectively. Based on these results, the H2SO4, BaCl2, and SGA methods are not recommended for detection of early pregnancy in the field.

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Seed germination test as an alternative urine-based non-invasive pregnancy test in alpacas (Vicugna pacos)

Cited by 2 publications

References 0 publications

Can Wheat Germination Test Be Used to Predict Pregnancy in Ewes?

Can Wheat Germination Test Be Used to Predict Pregnancy in Ewes?

Evaluation of Sulfuric Acid, Barium Chloride, and Seed Germination Assay Methods as Early Pregnancy Detection Instruments in Cattle

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