Seed Germination of North American Orchids. I. Native California and Related Species of Calypso, Epipactis, Goodyera, Piperia, and Platanthera

Arditti, Joseph; Michaud, Justine D.; Oliva, Allison P.

doi:10.1086/337245

Cited by 64 publications

(46 citation statements)

References 14 publications

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“…MS basal medium fortified with different plant growth hormones improve the nutritional status of the basal medium and favored earlier seed germination and protocorm formation. The result is in conformity with the findings of Arditti et al, (1981).…”

Section: Resultssupporting

confidence: 92%

In vitro Mass Propagation of Endangered Terrestrial Orchid Phaius tankervilliae (L’Her.) Blume through Green Seed Pod Culture

Thokchom¹,

Maitra²,

Sharma³

2017

Int.J.Curr.Microbiol.App.Sci

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Section: Resultssupporting

confidence: 92%

In vitro Mass Propagation of Endangered Terrestrial Orchid Phaius tankervilliae (L’Her.) Blume through Green Seed Pod Culture

Thokchom¹,

Maitra²,

Sharma³

2017

Int.J.Curr.Microbiol.App.Sci

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“…In our cultures, a treatment with 12-h photoperiod favored early onset of germination with better germination percentage as compared with complete darkness. In many terrestrial orchids, light is a limiting factor for seed germination [17,34,40] and the photoperiod responses in the germination of terrestrial orchid seeds are species specific [41].…”

Section: Discussionmentioning

confidence: 99%

Asymbiotic seed germination and multiplication of an endangered orchid – Paphiopedilum venustum (Wall. ex Sims.)

Kaur¹,

Bhutani²

2016

Acta Soc Bot Pol

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The present study was intended to facilitate ex situ conservation of Paphiopedilum venustum, a highly floriferous endangered terrestrial orchid species. A protocol was established for in vitro propagation and shoot multiplication. The cultures were initiated through asymbiotic seed germination technique, using undehisced and dehisced capsules. Four defined asymbiotic orchid seed germination media (terrestrial orchid medium, modified terrestrial orchid medium, Malmgren modified terrestrial orchid medium, Knudson C medium) were evaluated for their effectiveness in achieving maximum seed germination and early seedling development. The effect of darkness and 12-h photoperiod was also tested. Optimum seed germination, i.e., 82.7% was achieved on modified terrestrial orchid medium under a 12-h photoperiod using seeds from undehisced capsules. Shoot multiplication was accomplished using organic [peptone (1.0, 2.0 g L )] growth supplements. Peptone at 1.0 g L −1 was the most effective in multiplying the shoots. Plantlets were acclimatized in the greenhouse with 80% survival frequency.

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“…Regenerated plants from nodes produced flower 16 months after adaptation but regenerated plantlets from seeds showed longer juvenility and produced flower after 21 months and regenerated plants from leaves produced flower 18 month after adaptation to environment. Several studies have shown that the seeds from immature capsules can germinate much earlier (Arditti et al 1981, Stancato et al 1998, the use of immature seed capsules as a seed source shortened the reproductive cycle by approximately 2 to 2.5 months. Also, the utilization of seeds from unripe capsules allows to avoid the negative influences of sterilized substances, in this experiment we used unripe capsule too which produced 5 months after pollination.…”

Section: Resultsmentioning

confidence: 99%

Efficient in vitro culture protocols for propagating Phalaenopsis Cool Breeze

Balilashaki

Naderi

Kalantari

et al. 2015

Plant Tissue Cult. & Biotech.

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Phalaenopsis orchids have high economic value in the floriculture industry as cut flowers and potted plants throughout the world. Plant tissue culture technology is being widely used for large scale plant multiplication of Phalaenopsis to feed into this industry. In order to increase the efficiency of this technology, four experiments were undertaken: Plantlet regeneration from seeds, nodes or leaves, and hardening of the regenerated plants. In the first experiment, seed germination was examined in three media (half MS, Chen and Vacin-Went) of which the Chen medium had the best result (83.4%) in comparison to the other two media. In the second experiment, nodes on the flower stalk were studied for their shoot formation potential to different concentrations of BA and NAA, The highest frequency of shoot regeneration was achieved on MS containing 4 mg/l BA and 1 mg/l NAA while in vitro derived leaves formed clusters of somatic embryos directly when cultured on MS containing TDZ at different concentrations (0.5, 1, 2 and 3 mg/l). The embryos turned green and developed into protocorm-like bodies after 7 weeks of culture followed by plantlet regeneration. The highest plantlet regeneration from the leaf-derived embryos was obtained from MS supplemented with 3 mg/l TDZ. Finally, regenerated plants from (seeds, nodal explants and leaves) were compared in two medium for hardening, regenerated plant from nodal explants showed the highest survival rate (100%) on the medium containing cocopeat, coal, industrial cartridge and the bites of yonolit (1 : 1 : 2 : 4).

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Seed Germination of North American Orchids. I. Native California and Related Species of Calypso, Epipactis, Goodyera, Piperia, and Platanthera

Cited by 64 publications

References 14 publications

In vitro Mass Propagation of Endangered Terrestrial Orchid Phaius tankervilliae (L’Her.) Blume through Green Seed Pod Culture

In vitro Mass Propagation of Endangered Terrestrial Orchid Phaius tankervilliae (L’Her.) Blume through Green Seed Pod Culture

Asymbiotic seed germination and multiplication of an endangered orchid – Paphiopedilum venustum (Wall. ex Sims.)

Efficient in vitro culture protocols for propagating Phalaenopsis Cool Breeze

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Seed Germination of North American Orchids. I. Native California and Related Species of Calypso, Epipactis, Goodyera, Piperia, and Platanthera

Cited by 64 publications

References 14 publications

In vitro Mass Propagation of Endangered Terrestrial Orchid Phaius tankervilliae (L’Her.) Blume through Green Seed Pod Culture

In vitro Mass Propagation of Endangered Terrestrial Orchid Phaius tankervilliae (L’Her.) Blume through Green Seed Pod Culture

Asymbiotic seed germination and multiplication of an endangered orchid – Paphiopedilum venustum (Wall. ex Sims.)

Efficient in vitro culture protocols for propagating Phalaenopsis Cool Breeze

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Efficient in vitro culture protocols for propagating Phalaenopsis Cool Breeze