Seed germination of Allochrusa gypsophiloides(Caryophyllaceae), an endemic species from Central Asia and Kazakhstan

Abstract

The results of evaluating the laboratory seed germination of endemic Allochrusa gypsophiloides (Turkestan soap root), depending on storage conditions in combination with gibberellic acid treatment (GA3), are presented. In dry storage, control seeds were characterised by a long after-ripening period and a fluctuating germination behaviour upon removal from storage, with a maximum value of 23%. The sensitivity of seeds to GA3 during dry storage varied significantly, with two germination peaks at 5-7 months, and 12 months (37.5 and 50% germination, respectively). Cold stratification and cryo-preservation accelerated seed after-ripening, promoted germination synchronisation and increased seed sensitivity to GA3. The cold stratification of seeds increased germination four months earlier than during dry storage. GA3 increased germination from 16.7 and 18.3% for the control to 37.5 and 45% for seeds cryopreserved for 5 and 12 months, respectively. We recommend cryopreserving Turkestan soap root seeds to avoid viability loss and to then germinate the seeds after pretreatment with GA3.

Total Content of Saponins, Phenols and Flavonoids and Antioxidant and Antimicrobial Activity of In Vitro Culture of Allochrusa gypsophiloides (Regel) Schischk Compared to Wild Plants

“…Each analyzed group included plant material from roots or aboveground parts from three plants at the same vegetation phase, having close root morphometric parameters: length, 25-30 cm; raw weight, 1.2-1.5 kg; diameter, 8-11 cm. The seed material collected during the expeditionary survey of the natural populations in the Turkestan region [68] was used for TSR introduction ex situ. Seed sowing was preliminarily carried out in the fall of 2014 at the experimental site in the southeast of Kazakhstan (Almaty region).…”

“…The cultivation cycle on the inducing MS medium was 30 days with further microcutting and double passaging on a similar medium; subsequent passages were carried out on the medium without growth regulators. Seeds of natural populations were deposited under liquid nitrogen conditions to preserve germination [68] and were used as the starting material for isolated root cultures. Seeds were pretreated with 0.01% gibberellic acid solution for 2 h and then transferred to Knop's nutrient medium to obtain aseptic seedlings under stationary conditions in the light room.…”

Morphology of seeds of some species of rare and endemic plants of Kazakhstan

“…Скульптура поверхности, окраска и форма семян являются значимыми признаками для видовой диагностики семенных растений. В Казахстане проводится очень мало исследований по изучению морфологии и качества семян [4][5][6][7], которые ограничиваются одним или несколькими видами растений.…”