Seed germination and storage conditions of Ilex embelioides Hook.f. (Magnoliopsida: Aquifoliales: Aquifoliaceae), a threatened northeastern Indian species

Abstract

Ilex embelioides Hook.f. is a threatened tree species endemic to northeastern India. The species propagates naturally through seeds but shows a brief dormancy period. The present study was carried out to assess the germination behaviour, and identify the suitable storage conditions of I. embelioides seeds. Results revealed that warm stratification for 30 days could effectively break dormancy. Seeds pre-treated with gibberellic acid (GA3, 2,000 mg L-1) showed highest germination (63.89 ± 0.91%) as compared to other concentrations of GA3 as well as potassium nitrate (KNO3). Highest in vitro seed germination percentage (65.56 ± 2.92%) was recorded on (Murashige and Skoog) MS medium containing 10 mg L-1 GA3. The viability of the seeds declined with storage period irrespective of its storage condition. After 30 days, highest viability (54.72 %) was observed in seeds stored in moist sand at 25°C. Therefore, application of GA3 and warm stratification (25 ± 1°C) along with a substrate (moist sand) for 30 days may be considered as ideal conditions for effective germination and storage of I. embelioides seeds. This study can also be used for mass propagation of the species for reintroduction in the wild.