Seed dressing with mefenpyr-diethyl as a safener for mesosulfuron-methyl application in wheat: The evaluation and mechanisms

Abstract

Mesosulfuron-methyl is always applied by foliar spraying in combination with the safener mefenpyr-diethyl to avoid phytotoxicity on wheat (Triticum aestivum L.) cultivars. However, it was observed that the tolerance of Tausch’s goatgrass (Aegilops tauschii Coss.) to mesosulfuron-methyl significantly increased in the presence of mefenpyr-diethyl by performing bioassay. This confirmed phenomenon may lead to overuse of mesosulfuron-methyl and weed resistance evolution in field conditions. Therefore, we tested the effect of wheat seed dressing with mefenpyr-diethyl as a possible alternative and disclosed the underlying mechanisms by herbicide dissipation study, enzymatic analysis and transcriptome profiling. The results suggest that increase of ALS activity, enhancement of metabolic processes, and other stress responses are crucial for the regulation of herbicide detoxification induced by mefenpyr-diethyl. Additionally, transcription factors such as AP2/ERF-ERF, bHLH, NAC, and MYB, and protein kinase such as RLK-Pelle_DLSV might play vital regulatory roles. The current study has important implications for mesosulfuron-methyl application in wheat field to control Tausch’s goatgrass and provides a comprehensive understanding of the protective effect of mefenpyr-diethyl.

Research Progress on the Action Mechanism of Herbicide Safeners: A Review

“…The ALS-inhibitor herbicide mesosulfuron-methyl significantly inhibited ALS enzyme activity in wheat. At the same time, MD was able to resist this inhibition . Fenoxaprop-ethyl (FE) impedes maize growth by inhibiting ACCase activity in maize.…”

“…UGTs, the most common glycosyltransferase in plant metabolic detoxification, inactivate toxic compounds by glycosylation modification of various functional groups in herbicide molecules, storing them as inactive coupling. Glycosylated metabolites increase polarity and reduce toxicity and are more easily transported to the vacuole for isolation and breakdown from xenobiotics. , Safeners enhance the glycosylation of herbicides in protected plants from herbicide toxicity. , The importance of UGT72B1 was investigated in the degradation of exogenous compounds in A. thaliana by gene knockout. It was shown in the results that the uptake and metabolism rate of 3,4-dichloroaniline was significantly reduced in the A. thaliana mutant obtained when knocking out UGT72B1 .…”

“…At the same time, MD was able to resist this inhibition. 24 Fenoxaprop-ethyl (FE) impedes maize growth by inhibiting ACCase activity in maize. In contrast, the safener MD significantly increased ACCase activity in maize shoots and roots to alleviate the FE injury to maize.…”

“…11,57 Safeners enhance the glycosylation of herbicides in protected plants from herbicide toxicity. 24,58 The importance of UGT72B1 was investigated in the degradation of exogenous compounds in A. thaliana by gene knockout. It was shown in the results that the uptake and metabolism rate of 3,4-dichloroaniline was significantly reduced in the A. thaliana mutant obtained when knocking out UGT72B1.…”

Advances in the Mining of Disease Resistance Genes from Aegilops tauschii and the Utilization in Wheat

“…(3) Control of malignant weeds in crop fields: In the current production, the herbicide mesosulfuron–methyl is mainly used to control Aegilops tauschii in wheat fields, and it is often used in combination with the safener mefenpyr–diethyl [ 42 ]. However, Yuan et al [ 43 ] found that the tolerance of Aegilops tauschii to mesosulfuron–methyl was significantly increased in the presence of mefenpyr–diethyl by performing a bioassay, and they proposed that seed dressing with mefenpyr–diethyl could replace spraying to improve the resistance of wheat to mesosulfuron–methyl and enhance the control effect on Aegilops tauschii .…”