Seed Deterioration Study in Pea, Using Accelerated Ageing Techniques

Jatoi, Shakeel Ahmad; Afzal, Muhammad; Nasim, Shahzad; Anwar, Rashid

doi:10.3923/pjbs.2001.1490.1494

Cited by 28 publications

(17 citation statements)

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“…According to Kapoor et al (2011), seed deterioration is the loss of seed quality (viability and vigour) due to the adverse effect of environmental factors and is a natural process which involves cytological, physiological and biochemical changes. The rate of deterioration fluctuates critically from one species to another and also among varieties of the same species (Jatoi et al, 2001;Jyoti and Malik, 2013). The deterioration is evident in the reduction in percentage germination, production of weak seedlings, loss of vigour, seed become less viable and ultimately seed death (Murthy et al, 2000;Tilebeni and Golpayegani, 2011).…”

Section: Discussionmentioning

confidence: 99%

Longevity and vigour of pigeon pea (Cajanus cajan (L.) Millsp.) seed stored under humid tropical ambient conditions

Adebisi

Kehinde

Oladipo

et al. 2019

AAS

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Longevity and vigour of pigeon pea (Cajanus cajan (L.) Millsp.) seed stored under humid tropical ambient conditionsAbstract: Seeds of 20 pigeon pea (Cajanus cajan (L.) Millsp.) genotypes were evaluated for seed longevity and vigour under humid ambient conditions. Cleaned seeds of each genotype were packed into a polyethylene bag, the packaged lot was electrically sealed and thereafter placed in a seed store under ambient conditions (30 ºC, RH 75 %). Seed samples were withdrawn at 0, 60, 120, 180 and 240 days after storage for seed quality parameters evaluation. The experiment consisted of two factors which were genotype and storage duration and was a laid out in a completely randomized design with three replications. Data were collected on rate of seed germination, seed viability, seedling length, seedlings fresh mass, seedlings dry mass and seedling vigour index. Data collected were subjected to analysis of variance and significant treatment means were separated using Tukey's HSD test at 5 % probability level. PROBIT modelling was also used to predict the seed longevity of stored pigeon pea. Significant differences were observed in all seed quality attributes evaluated among the 20 pigeon pea genotypes and storage time except seedling fresh mass. Seed quality attributes decreased significantly with increasing storage periods. Genotypes NSWCC-18A, NSWCC-24, NSWC-34 and NSWCC-29A were identified to be superior for most of the seed quality attributes evaluated. PROBIT modelling result revealed that genotype NSWCC-29b had the highest storage life (16.28 months) and the highest storage potentials in terms of seed viability and other seed quality attributes of all seed lots. Dolgoživost in vitalnost semen kajana (Cajanus cajan (L.) Millsp.) shranjenih v vlažnih tropskih razmerahIzvleček: Dolgoživost in vitalnost semen 20 genotipov kajana (Cajanus cajan (L.) Millsp.) sta bili ovrednoteni v vlažnih tropskih razmerah. Očiščena semena vsakega od genotipov so bila shranjena v zaprte polietilenske vrečke, ki so bile nameščene v hrambo semen pod okoljskimi razmerami (30 ºC, RH 75 %). Vzorci semen so bili odvzeti po 0, 60, 120, 180 in 240 dnevih za ovrednotenje parametrov kakovosti. Poskus je bil dvofaktorski, kjer je bil prvi dejavnik genotip, drugi pa trajanje hrambe in je bil izveden kot popolni naključni poskus s tremi ponovitvami. Zbrani so bili podatki o kalivosti semen, njihovi viabilnosti, dolžini sejank, sveži in suhi masi sejank in njihovem vitalnostnem indeksu. Podatki so bili ovrednoteni z analizo variance, poprečja značilnih obravnavanj so bila ločena s Tukeyevim HSD testom pri verjetnosti 5 %. Za predvidevanje dolgoživosti shranjenih semen kajana je bil uporaben PROBIT model. Ugotovljene so bile značilne razlike v vseh ovrednotenih kakovostnih parametrih semen med vsemi 20 genotipi kajana in med časi hrambe, z izjemo sveže mase sejank. Kakovostni parametri semen so se s časom shranjevanja značilno zmanjševali. Genotipi NSWCC-18A, NSWCC-24, NSWC-34 in NSWCC-29A so bili prepoznani kot najboljši v vseh ovrednotenih...

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Section: Discussionmentioning

confidence: 99%

Longevity and vigour of pigeon pea (Cajanus cajan (L.) Millsp.) seed stored under humid tropical ambient conditions

Adebisi

Kehinde

Oladipo

et al. 2019

AAS

View full text Add to dashboard Cite

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“…There are studies showing the suitability of artificial/ accelerated ageing in studying the pattern of seed deterioration and storage assessment in various crops (Kibinza et al, 2006;Jatoi et al, 2001;Scialabba et al, 2010). The most powerful tool to predict the storability involves seed germination responses after the accelerated Accelerated Ageing Mediated Seed Longevity Prediction and Assessment of Seed Deterioration Pattern through... ageing (Delouche and Baskin, 1973).…”

Section: Prediction Of Seed Storabilitymentioning

confidence: 99%

Accelerated Ageing Mediated Seed Longevity Prediction and Assessment of Seed Deterioration Pattern through 2D-Gel Electrophoresis in Chickpea (Cicer arietinum L.)

Patil

Sajjan

Biradarpatil

et al. 2021

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Background: Accelerated ageing is a method to assess seed storage performance by exposing the seeds to higher temperature and relative humidity to make seeds lose its vigour and viability quickly. The seed physiological parameters are then compared with the natural ageing to derive certain conclusions. But, the molecular pattern of seed deterioration under accelerated ageing varies compared to that of natural ageing.Methods: Chickpea, seed lots of variety JG-11 and Annigeri-1 were subjected to natural ageing in cloth bag with nine per cent initial seed moisture under ambient conditions. Simultaneously, representative sample of same seed lot were also aged at 41oC and 95±2% humidity up to 120 hours.Result: Both the ageing methods had significant negative effect on seed physiological and biochemical quality parameters. The germination potential of seeds tested after 48 hours of accelerated ageing (83.5%) was equal 10 months of natural ageing (85%) in JG-11, while also in Annigeri-1, 48 hours of accelerated ageing (78%) was nearly equal to 10 months of natural ageing (80%). The protein expression analyzed through 2D-PAGE at similar germination potential brought out by two independent ageing methods showed higher protein down regulation ratio (3.4) in accelerated ageing than in natural ageing (3.2) unveiling its rapidity in the seed deterioration process. Therefore, accelerated ageing can be used for predicting chickpea seed longevity.

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“…Once seeds with high viability become susceptible to ageing, they tend to suffer a loss of viability quickly afterwards, owing to the accumulation of ROS [53]. The reports of previous studies have suggested differences in the rate of seed deterioration in storage across species and among even cultivars of related species [57,58] and seed lots within a species [38] in relation to their condition of storage. For instance, Walters et al [59] reported variations in the rate of seed deterioration in terms of viability loss in about 276 species and 207 cultivars from 42 species, including lettuce under cold storage in gene banks.…”

Section: Effect Of Exogenous Application Of Antioxidants On Photosynthetic Rate Gas Exchange and Photochemistry Of Seedlings Produced Fromentioning

confidence: 99%

Exogenous Antioxidants Enhance Seedling Growth and Yield of Artificially Aged Cabbage and Lettuce Seeds

et al. 2021

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Aged seeds exhibit compromised vigour in terms of germination, seedling emergence and growth, but this can to some extent be alleviated by invigoration treatments before sowing. This study aimed to investigate ageing rates and patterns in cabbage (Brassica oleraceae) and lettuce (Lactuca sativa) seeds and whether the beneficial effects of invigorating aged seeds with exogenous antioxidants translate to enhanced seedling performance. Seeds were artificially aged to 25% viability before soaking in 0.4 mM glycerol, 0.6 mM GSH and 0.2 mM trolox for cabbage, and 0.6 mM glycerol, GSH and trolox for lettuce; deionised water served as a control. After 14 days of sowing, seedling emergence percentage, mean emergence time, mean daily emergence, and time taken to 25% emergence were computed. Seedling vigour index, root and shoot dry weight, root:shoot ratio, leaf area, leaf area ratio, and leaf chlorophyll content were assessed 6 weeks after sowing. Furthermore, the photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (E), and chlorophyll fluorescence were measured 6 weeks after sowing. Notably, ageing resulted in the loss of seed vigour and viability at higher rates in lettuce than cabbage. Seed pretreatment with glycerol promoted seedling growth in both species and shoot dry weight in lettuce, while glycerol and GSH enhanced Pn, Gs and E in lettuce. Trolox also enhanced Pn and E in lettuce. The beneficial effects of the antioxidant treatments are thought to be associated with the protection of photosystems from oxidative stress and/or stimulation of enzymes involved in photosynthesis, possibly through an enhanced antioxidant defence system during the early development stages when seedlings are particularly vulnerable to stress.

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Seed Deterioration Study in Pea, Using Accelerated Ageing Techniques

Cited by 28 publications

References 1 publication

Longevity and vigour of pigeon pea (Cajanus cajan (L.) Millsp.) seed stored under humid tropical ambient conditions

Longevity and vigour of pigeon pea (Cajanus cajan (L.) Millsp.) seed stored under humid tropical ambient conditions

Accelerated Ageing Mediated Seed Longevity Prediction and Assessment of Seed Deterioration Pattern through 2D-Gel Electrophoresis in Chickpea (Cicer arietinum L.)

Exogenous Antioxidants Enhance Seedling Growth and Yield of Artificially Aged Cabbage and Lettuce Seeds

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