Seed coating with biological agents to improve the quality of rice seeds contaminated with blast pathogens and increase seedling growth

Abstract

Abstract. Palupi T, Riyanto F. 2020. Seed coating with biological agents to improve the quality of rice seeds contaminated with blast pathogens and increase seedling growth. Biodiversitas 21: 683-688. The aim of this research were (1) to evaluate the compatibility of five biological agents (SP21, SP31, RP21, TP12, and TP11), (2) to evaluate the physical and physiological quality of rice seeds contaminated with Pyricularia grisea after being treated with seed coating enriched with biological agents, (3) to evaluate the effectiveness of seed coating on the incidence of blast disease and seedling growth. The first experiment tested the compatibility of five bacterial antagonists on the PSA plates. There were eight treatments in the second experiment namely seed coating enriched with SP21+SP31; SP21+RP21; SP21+TP12; SP31+RP21; RP21+TP11; fungicides; positive control (rice seeds contaminated with blast), and negative control (healthy seeds), and seven treatments in the third experiment which were the same as in experiment 2 without negative control. Parameters observed in the second experiment were: seed germination, vigor index, and growth rate, while parameters observed in the 3rd experiment were the incidence rate of blast disease and plant height. The results of experiment 1 showed that isolates SP21+SP3, SP21+RP21, SP21+TP12, SP31+RP21, and RP21+TP11 had good growth compatibility without antagonism, and they can be used as biocontrol agents. Results of experiment 2 showed that seed coating enriched with SP21+SP31 isolates has the potential to improve the physical and physiological quality of the seeds. Results of experiment 3 showed that seed coating enriched with RP21+TP11 had no incidence of blast disease up to 4 weeks after planting.

Microbial seed coating: An attractive tool for sustainable agriculture

“…Results showed that treatments of biological control boosted seed vigor, and reduced infection of Xanthomonas oryzae pv. Oryzae in the seed [ 207 ]. To reduce aflatoxin contamination in corn kernels, the biocontrol techniques were performed via film coating.…”

“…Coating seeds of Triticum durum with sixty-two rhizosphere and endophytic bacterial strains caused the blockage of growth and germination Fusarium culmorum [ 172 ]. It has been proved that biological agents used in rice seed coating could improve the seed quality, seedling growth and decrease the blast disease to 0% [ 207 ]. Seed coating with entomopathogenic fungi Metarhizium sp., and Beauveria sp.…”

Microbial seed coating: an attractive tool for sustainable agriculture

“…Results showed that treatments of biological control boosted seed vigor, and reduced infection of Xanthomonas oryzae pv. Oryzae in the seed (Palupi and Riyanto 2020). To reduce aflatoxin contamination in corn kernels, the biocontrol techniques were performed via film coating.…”

“…Coating seeds ofTriticum durum with sixty-two rhizosphere and endophytic bacterial strains caused the blockage of growth and germinationFusarium culmorum (Mnasri et al 2017). It has been proved that biological agents used in rice seed coating could improve the seed quality, seedling growth and decrease the blast disease to 0% (Palupi and Riyanto 2020). Seed coating with entomopathogenic fungi Metarhizium sp., and Beauveria sp.…”

Invigorasi Benih Padi Menggunakan Mikroba Fungsional

“…Pseudomonas diminuta A6 dan Bacillus subtillis 5/ B (Palupi et al, 2016); bakteri isolat 4A yang teridentifikasi Paenobacillus alvei (Purwaningsih et al, 2019); bakteri isolat WH24 dan WH31C (Sinambela, 2019); dan isolat SP21 + SP31 (Palupi dan Riyanto, 2020). Oleh karena itu pada penelitian ini dilakukan invigorasi melalui perendaman yang diintegrasikan dengan mikroba fungsional.…”