Seed bulb size influences the effects of exogenous brassinolide on yield and quality of Pinellia ternata

Abstract

In recent years, natural Pinellia ternata populations of have gradually been exhausted, while the cultivated yield has been limited due to lack of research and uncertain climate condition. Therefore, it is necessary to explore methods of improving yield and quality in P. ternata using brassinolide (BR) treatments and choice of a suitable seed bulb size.• This article reports the effects of BR and two seed bulb sizes (diameter: 0.5-1.0 cm and 1.0-1.5 cm) on active and nutrient components and antioxidant activity in P. ternata. The experiment included six levels of BR (0, 0.05, 0.10, 0.50, 1.00 and 2.00 mg l À1 ).• The tuber yield of the two seed bulb sizes and bulbil yield of small seed bulbs increased 5.67%, 22.66% and 69.23% by day 105 after 0.50 mg l À1 BR treatment, compared with the control. On day 105, only 0.05 mg l À1 BR increased scores in principal components analysis (PCA) in tubers of small seed bulbs by 167.29%, and 0.05 and 0.50 mg l À1 BR increased PCA score in bulbils of large seed bulbs by 145.66% and 252.97%, respectively, compared with the control. Significant BR 9 seed bulb size interactions were found on yield and quality of P. ternata.• The results indicate that BR effects on yield and quality of tubers and bulbils of P. ternata are not only related to BR concentration but also to seed bulb size.

Regulation Mechanism of Exogenous Brassinolide on Bulbil Formation and Development in Pinellia ternata

“…Bulbils are important vegetative propagation organs in P. ternata . Our previous study showed that BR was involved in bulbil formation and development ( Guo et al, 2021a , b ). However, it is not clear about the molecular mechanism of BR regulating bulbil formation and development.…”

Brassinolide Soaking Reduced Nitrite Content and Extended Color Change and Storage Time of Toona sinensis Bud during Low Temperature and Near Freezing-Point Temperature Storage

“…β-sitosterol content was measured based on the previous report with minor modifications [ 53 ]. Dried samples were immersed in an ethyl acetate solution for 12 h. After centrifuging, the supernatant (4 mL) was evaporated in a water bath.…”

“…The total alkaloid was assayed according to the report with tiny modifications [ 53 ]. Dried samples (0.2 g) were extracted with ammonium hydroxide (0.5 mL, 25%) and chloroform (5 mL) at 37 °C for one hour.…”