Seed biology of Coscinium fenestratum (Gaertn.) Colebr.- A critically endangered medicinal plant of Western Ghats

Ramasubbu, Raju; Ch, Avtar; Prabha, R. Uma; Kumuthakalavalli, R.

doi:10.5897/jmpr11.558

Cited by 6 publications

(1 citation statement)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, various techniques are adopted earlier for breaking the dormancy (Anil Kumar et al, 2010;Ramasubbu et al, 2012;Warakagoda and Subasinghe, 2015). The same method is tried in this experiment and is resulted GA 3 at dose of 2500 ppm showed better result than others.…”

Section: Seed Germinationmentioning

confidence: 99%

Effect of plant hormones and zinc sulphate on rooting and callus induction in in vitro propagated Coscinium fenestratum (Gaertn.) Colebr. stem and their role in estimation of secondary metabolites

Das¹,

Dang²,

Sivaraman³

et al. 2018

View full text Add to dashboard Cite

Coscinium fenestratum (CF) (Gaertn.) Colebr. is a hard woody climber medicinal plant belongs to the family, Menispermaceae. The plant is commonly known as tree turmeric because the stem content yellow berberine, the major active constituent. The plant is widely available in Western Ghats region but became endangered due to over exploitation and very slow germination rate. Hence, the alternate in vitro tissue culture has established for reduced germination rate and to extract more a mount of plant constituents from the stem callus. Full strength MS medium supplemented with 2, 4-D at 0.1 mg/l and kinetin at 2 mg/l gave callus growth in 46 days and the growth mechanism is observed first time through SEM study of callus. Thereafter, rooting of callus occurred in IBA and kinetin in combinations in half strength MS medium whereas direct rooting of stem occurred with IBA and zinc sulphate (IBA at 2 mg/l and ZnSO 4 at 3 mg/l) supplemented in half strength MS media along with coconut water, within 18 days. Thereafter, extracted callus is identified with TLC, followed by estimated with HPLC and HPTLC and resulted methanol extract of CF showed higher content of berberine than aqueous extract.

show abstract

Section: Seed Germinationmentioning

confidence: 99%